Composite chip for detecting autoimmune antibody of diabetes mellitus, and preparation and detection method thereof

An autoimmune antibody and composite chip technology, applied in the field of biomedical research and clinical application, can solve the problems of high detection cost and inaccurate diagnosis of diabetes typing, achieve high sensitivity, avoid false positive and false negative detection results Produce, easy to popularize the effect

Inactive Publication Date: 2003-06-11
XIAN BESTEDIT BIOMEDICAL SERVICES
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Problems solved by technology

[0006] The invention aims to solve the problems of inaccurate diagnos

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  • Composite chip for detecting autoimmune antibody of diabetes mellitus, and preparation and detection method thereof
  • Composite chip for detecting autoimmune antibody of diabetes mellitus, and preparation and detection method thereof
  • Composite chip for detecting autoimmune antibody of diabetes mellitus, and preparation and detection method thereof

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[0049] The invention relates to a composite chip for detecting diabetes autoimmune antibodies, its preparation and detection method, and is mainly used for detecting four important indexes in auxiliary diagnosis of diabetes: ICAs, GADA, ICA512 / IA-2 and IAA. The present invention will be further described by the following examples, but it should not be understood that the scope of protection is limited to the following examples.

[0050] (1) A protein chip for detecting autoimmune antibodies in diabetes, comprising a housing 1 and a detection microarray 3, the housing 1 is a glass sheet with a specification of 22mm×75mm and a thickness of 1mm, and 78 pieces are arranged on the upper surface of the glass sheet Reaction pool 2, said reaction pool 2 is an inverted table shape with a surface side length of 2.5 mm, a bottom side of 2.0 mm, and a depth of 0.5 mm. A detection microarray 3 is arranged in each reaction pool 2, and the detection microarray 3 consists of four areas of 1mm...

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Abstract

Glass thin slice with the multiple reaction ponds is set up on the outer casing of the compound chip for detecting oneself immunity antibody of diabetes. the detecting micro array is built in each pond. Four different kinds of protein solid phase detecting pieces in mini type with sample specification size constitute each detecting micro array. These detecting pieces are the piece of the islet cells, the piece of GAD protein, the PTP protein and the Insulin protein. First, these detecting pieces are prepared, then these pieces are assembled. The detecting method includes two steps: first, the indirect fluorescence method is used; then, the biochip laser co-focusing scanner determines the result. Comparing with the prior art the invention possesses the advantages of the accurate clinical analysis and the low cost.

Description

Technical field: [0001] The invention relates to the field of biomedical research and clinical application, in particular to a composite chip for detecting autoimmune antibodies in diabetes and its preparation and detection methods. Background technique: [0002] Diabetes is currently one of the most serious diseases that endanger human health and quality of life. Its main symptom is a persistent increase in blood sugar concentration, and it endangers health through a variety of serious complications. According to the International Diabetes Federation (KDF) on the current situation of global diabetes and the latest forecast of the World Health Organization (WHO) on the incidence of diabetes, it is pointed out that 130 million people with type II diabetes have been diagnosed in the world, and more than 40 million people in my country. It is estimated that by 2025, the number of diabetic patients in the world will exceed 300 million, and the total number of diabetic patients i...

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Application Information

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IPC IPC(8): G01N21/64G01N33/53G01N33/533G01N33/543G01N33/68
Inventor 刘镭罗毅王伟华
Owner XIAN BESTEDIT BIOMEDICAL SERVICES
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