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Method for detecting SARS virus and kit thereof

A pneumonia virus, a typical technology, applied in the direction of biochemical equipment and methods, microbial measurement/testing, fermentation, etc., to achieve the effect of rapid gene detection and early rapid diagnosis

Inactive Publication Date: 2003-10-22
HUADA GENE RES & DEV CENT HANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is currently no effective immunochemical detection method for the virus, and it can only be diagnosed based on clinical symptoms (high fever, dry cough, shadows in lung X-ray detection, etc.), which are greatly limited and can only be made after the onset of the infection. Diagnosis, and not easy to distinguish from other pneumonia and common influenza

Method used

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  • Method for detecting SARS virus and kit thereof

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Experimental program
Comparison scheme
Effect test

Embodiment

[0020] 1. Take biological samples from suspicious patients and extract RNA;

[0021] 1) Take the serum, secretion or tissue of suspicious patients, add 50 μl of PBS buffer, 160 μl of solution D, 160 μl of phenol, 32 μl of chloroform and 9 μl of 4.0 M (PH=3.8) sodium acetate.

[0022] Solution D contains: 2.5 grams of guanidine isothiocyanate, 1.76 ml of 0.75M sodium citrate (PH7.0), 2.46 ml of 10% sodium lauryl creatine, 29.3 ml of water, and 7.2 μl / ml before use of mercaptoethanol.

[0023] 2) Shake the mixture for 15 seconds and place on ice for 15 minutes.

[0024] 3) Centrifuge at 14000 rpm at 4°C for 20 minutes.

[0025] 4) Take the supernatant, add an equal volume of isopropanol, and place it on ice for 25 minutes.

[0026] 5) Centrifuge at 14000 rpm for 20-30 minutes at 4°C.

[0027] 6) The precipitate was washed twice with ethanol pre-cooled at -20°C, dried in vacuum, and dissolved in DEPC water.

[0028] 2. Add reverse primer and reverse transcriptase to synthesi...

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Abstract

The present invention discloses a method for clinical detection of SARS virus and its kit. According to the characteristic analysis of SARS virus genome sequence a specific region can be selected, and according to the characteristics of that said virus is of ribonucleic acid virus a specific primer sequence can be used and the reagent and techniques related to RT-PCR and PCR can be adopted so as to create a set of methods for quickly detecting SARS virus. Said invented method has higher accuracy, and provides its diagnosis method and its kit.

Description

technical field [0001] The invention relates to a method for detecting atypical pneumonia virus and a kit thereof. Background technique [0002] SARS virus is a virus that causes severe acute respiratory syndrome (Severe Acute Respiratory Syndrome, SARS), also known as atypical pneumonia. , 737 nucleotides. The disease is mainly transmitted through close-range air droplets and close contact. There is currently no effective immunochemical detection method for the virus, and it can only be diagnosed based on clinical symptoms (high fever, dry cough, shadows in lung X-ray detection, etc.), which are greatly limited and can only be made after the onset of the infection. Diagnosis, and not easy to distinguish from other pneumonia and common influenza. Contents of the invention [0003] The purpose of the present invention is to provide a method and kit for detecting SARS virus. [0004] The method for detecting atypical pneumonia vi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/34C12Q1/04C12Q1/68
Inventor 汪建王俊李蔚徐祖元林伟胡咏武张晓伟邓亚军杨唤明
Owner HUADA GENE RES & DEV CENT HANGZHOU
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