Application of snake venom L-amino acid oxidase in preparing AIDS treating medicine

A technology for treating drugs and amino acids, which is applied in the field of biomedicine to achieve the effects of reducing cytotoxicity, inhibiting virus replication, and improving therapeutic index

Inactive Publication Date: 2004-09-08
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The technical effect of this patented method for treating viruses caused by Human Immunodeficiency Virus type-1(HIV1) was that it could significantly decrease their ability to infect human T lymphocytes without causing harm or discomfort during treatment with these agents alone. This would make them more effective against retroviral diseases such as HTLV/AIDS.

Problems solved by technology

The technical problem addressed in this patented text relates to understanding how chemistry related proteins like histone laminating polypeptides play key roles during regulation processes associated with diseased states including cancer metastasis, hemorrhage, inflammations, etc. These complex interactions may affect drug discovery efforts due to lack of knowledge about them.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The snake venom L-amino acid oxidase used is isolated from the snake venom of Trimeresurus stejnegeri produced in China, or is a mutant (snake venom L-amino acid oxidase) artificially expressed through genetic engineering from the snake venom L-amino acid oxidase coding gene.

[0020] 1. Inhibitory effect of snake venom L-amino acid oxidase on HIV-1-induced syncytium formation in C8166 cells

[0021] Determination of cytotoxic activity of snake venom L-amino acid oxidase: on a 96-well flat-bottomed cell culture plate, the compound to be tested is diluted 5-fold with complete medium, and there are 5 dilutions in total, and 3 replicate wells are set for each dilution , 100 μl per well, and set normal cell control at the same time. Drop 3×10 per well 5 100 μl / ml of C8166 cells. Set at 37°C, 5% CO 2 Cultured in an incubator. After 72 hours, the toxic effect of the test compound on the cells was measured by the MTT method. CC50 is the drug concentration at which 50% of ...

Embodiment 2

[0052] Anti-HIV effect of snake venom L-amino acid oxidase in the presence of catalase:

[0053] The compounds inhibit tumor cell growth, inhibit HIV-1-induced syncytium formation in C8166 cells, and inhibit HIV virus replication as described in Example 1. EC50 is the concentration of drug that inhibits syncytia formation by 50%, P 24 Antigen EC50 is the drug concentration required to inhibit virus replication by 50%; CC50 is the drug concentration when 50% of the host cells are cytotoxic, and the therapeutic index (TI) is the ratio of CC50 / EC50

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Abstract

The present invention belongs to the field of biomedicine technology, and is the application of snake venom L-amino acid oxidase in preparing AIDS treating medicine. Snake venom L-amino acid oxidase may be obtained through separating the venom gland secretion of viper or cobra or through gene engineering expression of the encoded gene. Snake venom L-amino acid oxidase has obvious anti-HIV activity via inhibiting HIV to induce cell to form synplasm and inhibiting HIV duplication. Snake venom L-amino acid oxidase has high activity and anti-HIV mechanism different from that of available reeverse tgranscriptase inhibitor, such as AZT, and fusion blocking medicine, such as DS. On the other hand, the combined application of Snake venom L-amino acid oxidase and hydrogen peroxidase can reduce the cytotoxicity of Snake venom L-amino acid oxidase effectively and raise the treatment index obviously.

Description

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Claims

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Application Information

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Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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