Alocasia odora agglutinin protein gene and its application

A lectin and gene technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problem of no alocasia lectin protein gene cloning and other problems, and achieve the effect of reducing the application

Inactive Publication Date: 2004-10-20
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0012] Only its protease inhibitor gene has been cloned in calla lily, and there is no any report on the clone of calla lectin protein gene before the application of the present invention

Method used

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  • Alocasia odora agglutinin protein gene and its application
  • Alocasia odora agglutinin protein gene and its application
  • Alocasia odora agglutinin protein gene and its application

Examples

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Embodiment

[0052] 1. Cloning of the 3' end sequence of Alocasia lectin protein gene (AML)

[0053] The present invention uses RACE PCR to clone the 3' end sequence of the AML gene. First search Genbank and related literature, search for the nucleotide sequences and amino acid sequences of five lectins or similar proteins of other three plants under the family Araceae, which are closely related to the calla lily, and arrange and analyze the five to find out the The highly conserved three-segment region is used to design the gene-specific primers (GSP) necessary for 3'RACE PCR, and then perform 3'RACE PCR amplification to obtain the 3'-end sequence fragment, and then directly combine the PCR product with pMP 18-T vector The vectors are connected, transformed into E. coli competent cells, and the recombinant plasmids are screened for resistance through the LB plate containing AMP. A single colony is selected from the plate and cultured in shake flasks. The two enzymes used are E10RI and Hi...

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Abstract

A nucleotide (cDNA) sequence of Alocasia agglutinin protein, its coding amino acid (AA) polypeptide sequence, the process for using the gene to transform cotton or other plants, and its application in medicine or other fields are disclosed. Said gene is a member of mannose conjugated agglutinin gene family and is a constitutively expressed gene.

Description

technical field [0001] The invention relates to the cloning and application of the plant lectin protein gene, in particular to the cDNA sequence of the calla lily lectin protein gene and the encoded protein sequence. The invention further relates to the application of the calla lectin gene in plant insect resistance genetic engineering. Background technique [0002] Plant lectins are plant proteins that contain at least one non-catalytic domain and are capable of reversibly binding to specific mono- or oligosaccharides. [0003] Plant lectins have no common structural properties. Most lectins contain covalently bound sugar molecules and belong to glycoproteins. The composition of carbohydrates in lectin molecules is similar to that of other glycoproteins, mainly mannose, N-acetylglucosamine, galactose, fucose, and sialic acid, and a small amount of lectins contain xylose and arabinose. Some lectins do not contain covalently bound sugars. The prote...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/00C12N15/29
Inventor 侯学文朱亚然王捷潘科
Owner SOUTH CHINA AGRI UNIV
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