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Method for identifying pesticide resistance of dentes foxtail to sethoxydim

An identification method and a technology of watching Mai Niang, applied in the determination of the resistance of weed plants to herbicides, and monitoring the resistance of Mai Niang to Nachajing, can solve the problem of occupying a large greenhouse space, heavy workload, and difficult to achieve. Availability and other issues, to achieve the effect of low cost, small footprint, and detection of more population samples

Inactive Publication Date: 2004-11-10
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

But the defect of this method is that it is difficult to ensure the availability of the same agent between the herbicide and the plant (Tang Zhenghui et al., 1991), and it takes about 50 days in addition, the workload is large, and the greenhouse space is large.

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  • Method for identifying pesticide resistance of dentes foxtail to sethoxydim
  • Method for identifying pesticide resistance of dentes foxtail to sethoxydim
  • Method for identifying pesticide resistance of dentes foxtail to sethoxydim

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Embodiment Construction

[0030] In the spring of 2003, the newly collected seeds of Kanmai Niang were identified, and there were 6 populations in total. After the seed samples were soaked with 800ppm concentration of GA3 for 5 days under the condition of 4°C, the soaked seeds were sowed in a plastic petri dish with 2 layers of filter paper and a diameter of 9 cm. Add 7ml of Natura water solution to each dish, and the concentration gradient is 0, 0.05, 0.1, 0.2, 0.4, 0.8, 1.6, 3.2 mg / L Natura herbicide range. Culture in dark at 18°C, measure seedling length after 6 days, use SAS or SPSS statistical software for nonlinear regression, nonlinear regression equation: Y=k / (1+e b x c ), where x: herbicide dosage (ml / l); y: seedling length (cm); b, c: parameters of the curve; k: control seedling length (cm); GR 50 = e -g (g=b / c). Then the resistance ratio (R / S) was obtained, and the results are shown in Table 1. JXRI(R / S=32.9), JXRII(R / S=40.8) showed high resistance, HFRI(R / S=8.7), HFRII(R / S=9), LYR(R / S=...

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Abstract

The invention is a method to identify the resistance to NaBuJing weeding agent in KanMaiNiang, dipping the seeds of KanMaiNiang in GA3 at 800-1000ppm or 0.2% KNO3 at 4 deg.C for 5 days; after seeding, adding in 7mL of weeding agent at gradient concentration of 0-8mg / L, and after 6 days, measuring length of seedlings and using SAS or SPSS statistic software to make nonlinear recurrence to get GR50 and resistance ratio R / S, i.e. concentration in resistant population inhibition RGR50 / that in sensitive population inhibition SGR50; if R / S>10, high-resistance, if 3<R / S<10, medium-resistance and if R / S<3, sensitive. The determined result is consistent with that of the whole plant. It is fast, low-cost and suitable for identification of resistance to NaBuJing weeding agent in large-area KanMaiNiang, monitors the occurrence of resistant weeds in time and provides effective basis for field management.

Description

1. Technical field [0001] The invention discloses a method for identifying the drug resistance of magnifolia to Natura, which belongs to the method for measuring the resistance of weed plants to herbicides, and is specially used for monitoring the resistance of magnesia to Natura. 2. Technical Background [0002] Naturanet belongs to cyclohexanedione (CHD), and its target enzyme is acetyl coenzyme A carboxylase (acetyl coenzyme A carboxylase). [0003] CHD herbicides are the same as sulfonylurea herbicides with acetolactate synthase (ALS) as the target enzyme, because of a single target, continuous use is likely to cause weed resistance. In maize cell cultures, several resistant lines have been screened in the presence of Natura or Ghatneng. In the sensitive strains, compared with the unscreened strains, the inhibitory ability of Nabujing or Gaicao to ACCase activity decreased significantly (Wyse et al. 1992). According to Cotterman. (1992) report, after dipropa-methyl was...

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Application Information

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IPC IPC(8): G01N33/00
Inventor 王庆亚张守栋黄世霞董立尧
Owner NANJING AGRICULTURAL UNIVERSITY
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