Method for analyzing content of 2-ketone group-L-gulconic acid

An analytical method and technology for gulonic acid, applied in the field of chemical analysis, can solve the problems of chromatographic peak peak shape symmetry and resolution that have great influence, 2-keto-L-gulonic acid peak time is long, and operation time is increased. , to achieve the effect of long analysis time, cheap raw materials, and simplified work steps

Inactive Publication Date: 2005-03-23
TIANJIN UNIVERSITY OF TECHNOLOGY
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Problems solved by technology

[0005] In addition, high performance liquid chromatography (HPLC) has not been applied in industrial production, the main reason is that there are relatively large defects in this method: 1) samples need to use ZnSO 4 and K 4 Fe(CN) 6 3H 2 O is pretreated, which increases the operating time, 2) the temperature of the detector should be strictly controlled within 1°C, which is too harsh for actual production, and 3) the peak time of 2-keto-L-gulonic acid is longer , up to 32min, 4), mobile phase pH value, flow velocity, concentration have great influence on 2-keto-L-gulonic acid chromatographic peak shape symmetry and resolution

Method used

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  • Method for analyzing content of 2-ketone group-L-gulconic acid
  • Method for analyzing content of 2-ketone group-L-gulconic acid
  • Method for analyzing content of 2-ketone group-L-gulconic acid

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Embodiment

[0044] Embodiment: 1, preparation homogeneous solution and solution calibration:

[0045] Among them: 1) Prepare relevant uniform solutions, respectively prepare dichromate standard solution, thiosulfate solution, metal weak oxidant solution, KI solution, starch solution, dilute acid solution:

[0046] a) sodium thiosulfate solution: take by weighing 25g Na 2 S 2 o 3 ·5H 2 O, dilute to 1L with distilled water, put it in a brown bottle, and use it for calibration after the solution is uniform. The target value can be C(Na 2 S 2 o 3 )=0.1mol / L;

[0047] b) Starch solution: take 1 g of soluble starch and mix it with a small amount of cold water to make a paste, pour the resulting paste into 100 ml of boiling water, boil for several minutes, and cool to obtain a 1% starch solution;

[0048] c) Continue to prepare 20% KI solution and 20% dilute acid solution by conventional method.

[0049] 2) The calibration process is as follows:

[0050] a) The analytical balance accura...

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Abstract

The invention is an analyzing method for 2-ketone-L-gulong acid content. Its character lies in: the 2-ketone-L-gulong acid has redox reaction to metal weak oxidant, the excess metal weak oxidant oxidizes negative iodide ion into iodine, carries on titration to iodine generated in the solution with thiosulfate standard solution, works out the real weight of gulong acid in sample according to the chemical proportion in the reaction, thus measures the content percentage of gulong acid in sample. The merits of the invention lie in: 1) it solves the problem that the repetiliveness of data is bad with current technology to measure the content; 2) overcomes the complexity, hard and long period of HPLC; 3) it is accurate, the error is small, the operation is simple, and the material is cheap.

Description

(1) Technical field: [0001] The invention relates to a chemical analysis method, in particular to an analysis method for measuring the content of 2-keto-L-gulonic acid by iodometric method. (two) background technology: [0002] The chemical name of vitamin C (VC) is L-3-oxothreonolactone, also known as L-ascorbic acid. It is one of the 26 essential medicines jointly determined by the World Health Organization and the United Nations Industrial Development Organization. It is widely used In medicine, food, beverage, feed, chemical industry and other fields. It is the largest project of my country's medical technology export, and it has long occupied a very important position in the international pharmaceutical trade. China's vitamin C production accounts for 40% of the world's production, and it is also the first variety of my country's annual export earnings exceeding 100 million US dollars ( Since 1993), the first export of APIs exceeding 10,000 tons (since 1994), accounting...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 梁娅魏荣宝陈苏战张杰
Owner TIANJIN UNIVERSITY OF TECHNOLOGY
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