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Enzyme linked immunodiagnosis kit for muscle nebulousurine disease virus of Rhode's pond caray and detection method therefor

A technology of muscle white turbidity and diagnostic kits, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of no simple and easy farms, etc., and achieve the prevention of large-scale spread, high specificity and sensitivity, and simple methods easy effect

Inactive Publication Date: 2005-04-06
ZHEJIANG INST OF FRESH WATER FISHERIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, apart from visual inspection, there is no simple and easy method for farms to diagnose diseases and viruses

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0016] Embodiment: a kind of Macrobrachium rosenbergii muscle white turbidity virus detection reagent of the present invention consists of enzyme plate, virus positive control, virus negative control, antiviral monoclonal antibody, enzyme-labeled anti-mouse antibody, enzyme chromogenic solution, stop solution , sample dilution and washing solution, wherein the microplate plate is the microplate plate coated with anti-Macrobrachium rosenbergii Nodavirus antibody, the virus positive control is the grinding solution of Macrobrachium rosenbergii disease seedlings containing Macrobrachium rosenbergii Nodavirus, anti- The virus monoclonal antibody is a mouse monoclonal antibody with high specificity and binding ability to Macrobrachium rosenbergii Nodavirus. The enzyme color development solution uses tetramethylbenzidine (TMB), and the stop solution is 2M H 2 SO 4 , sample dilution and washing solution (X10) 10X phosphate buffered saline (PBS).

[0017] When measuring shrimp seedli...

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PUM

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Abstract

This invention relates to Roger swamp shrimp white muscle disease enzyme immune diagnose agent box and its measurement method. The diagnose agent box comprises enzyme label plate, virus positive comparison, virus negative comparison, anti-virus single clone antibody, enzyme label anti-mouse antibody, enzyme color develop agent, terminal liquid, and sample dilution and wash liquid, wherein the enzyme label plate is the one covered with antibody of Roger shrimp disease virus and the virus positive comparison is the Roger shrimp grinding fluid.

Description

technical field [0001] The invention relates to a virus ELISA diagnostic kit, in particular to an ELISA diagnostic kit for Macrobrachium rosenbergii muscle white turbidity virus and a detection method thereof. Background technique [0002] Macrobrachium rosenbergii muscle white turbidity disease, also known as white body disease and white tail disease, mainly harms giant macrobrachium rosenbergii seedlings. It occurs within 3-5 weeks after the shrimp seedlings are desalinated and stocked in ponds. The diseased shrimps show muscle white spots or white turbidity , Diseased shrimp can die in a large number in a relatively short period of time, especially for high-density cultured nursery ponds, the mortality rate can be as high as 40-90%. The disease was first discovered around 1996, and has now spread to major giant macrobrachium rosenbergii farms across the country, causing major economic losses in the macrobrachium rosenbergii farming industry. Sinc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/535G01N33/543G01N33/569G01N33/577
Inventor 钱冬刘问杨国梁
Owner ZHEJIANG INST OF FRESH WATER FISHERIES
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