Enzyme linked immunodiagnosis kit for muscle nebulousurine disease virus of Rhode's pond caray and detection method therefor
A technology of muscle white turbidity and diagnostic kits, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of no simple and easy farms, etc., and achieve the prevention of large-scale spread, high specificity and sensitivity, and simple methods easy effect
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[0016] Embodiment: a kind of Macrobrachium rosenbergii muscle white turbidity virus detection reagent of the present invention consists of enzyme plate, virus positive control, virus negative control, antiviral monoclonal antibody, enzyme-labeled anti-mouse antibody, enzyme chromogenic solution, stop solution , sample dilution and washing solution, wherein the microplate plate is the microplate plate coated with anti-Macrobrachium rosenbergii Nodavirus antibody, the virus positive control is the grinding solution of Macrobrachium rosenbergii disease seedlings containing Macrobrachium rosenbergii Nodavirus, anti- The virus monoclonal antibody is a mouse monoclonal antibody with high specificity and binding ability to Macrobrachium rosenbergii Nodavirus. The enzyme color development solution uses tetramethylbenzidine (TMB), and the stop solution is 2M H 2 SO 4 , sample dilution and washing solution (X10) 10X phosphate buffered saline (PBS).
[0017] When measuring shrimp seedli...
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