Pig monoamine oxidase A protein coding sequence
A porcine monoamine oxidase, monoamine oxidase technology, applied in the field of genetic engineering, can solve the problems of piglet death, piglet mental stress, influence on piglet weight gain, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0017] Cloning of cDNA Sequence of Porcine MAO-A Gene
[0018] The cDNA cloning process of porcine MAO-A gene is shown in the appendix figure 1 .
[0019] The specific operation method of the experimental method used in the cloning process is as follows:
[0020] 1. Isolation
[0021] The 30-day-old Dudu piglets were slaughtered in the pig farm, and the colon tissue samples were taken, quickly put into liquid nitrogen, frozen and stored in the -70°C refrigerator in the laboratory, ready to extract total RNA from the tissue.
[0022] 2. Total RNA isolation (Total RNA isolation)
[0023] (1) Preparation for RNA extraction
[0024] Glass products were soaked in 0.1M NaOH overnight, rinsed repeatedly with tap water, rinsed twice with distilled water, and baked at 180°C for 4 hours.
[0025] Soak the homogenizer and electrophoresis tank with 3% hydrogen peroxide for 20-30 minutes, and then rinse with 0.1% DEPC water. Since the uninactivated DEPC will affect the PCR reaction, i...
Embodiment 2
[0052] Homology Comparison of Coding Sequence of Porcine MAO-A Gene
[0053] Search and obtain the human, bovine, mouse, dog MAO-A gene coding protein sequence on GenBank, and sequence it with the SEQ ID NO.1 sequence obtained by the present invention obtained by cloning and sequencing, and perform sequence alignment on the molecular biology software DNAman source comparison. The comparison results showed that the identity of SEQ ID NO.1 and the CDS sequence of MAO-A gene of human, bovine, mouse, dog and other species was 91.26%, and the identity of amino acid sequence was 92.83%.
[0054] The sequences and symbols involved in the present invention are listed as follows:
[0055] Information on SEQ ID NO.1
[0056] Shanghai Jiao Tong University
[0057] Porcine MAO-A Gene Protein Coding Sequence
[0058]
[0059] 2004-05-17
[0060] 2
[0061] PatentIn version 3.1
[0062] 1
[0063] 1886
[0064] DNA
[0065] pig (sus scrofa)
[0066]
[0067] misc_feature ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com