Pig monoamine oxidase A protein coding sequence

A porcine monoamine oxidase, monoamine oxidase technology, applied in the field of genetic engineering, can solve the problems of piglet death, piglet mental stress, influence on piglet weight gain, etc.

Inactive Publication Date: 2005-06-22
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In modern pig production, the early weaning technology of piglets is an effective measure to improve productivity, and it has been widely used in pig production gradually. Weaning Stress Syndrome" means that the piglets are nervous, frightened, often howling, not eating or sleeping, indigestion, diarrhea, seriously affecting the weight gain of piglets and affecting subsequent growth, and severe piglets may die

Method used

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  • Pig monoamine oxidase A protein coding sequence

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Cloning of cDNA Sequence of Porcine MAO-A Gene

[0018] The cDNA cloning process of porcine MAO-A gene is shown in the appendix figure 1 .

[0019] The specific operation method of the experimental method used in the cloning process is as follows:

[0020] 1. Isolation

[0021] The 30-day-old Dudu piglets were slaughtered in the pig farm, and the colon tissue samples were taken, quickly put into liquid nitrogen, frozen and stored in the -70°C refrigerator in the laboratory, ready to extract total RNA from the tissue.

[0022] 2. Total RNA isolation (Total RNA isolation)

[0023] (1) Preparation for RNA extraction

[0024] Glass products were soaked in 0.1M NaOH overnight, rinsed repeatedly with tap water, rinsed twice with distilled water, and baked at 180°C for 4 hours.

[0025] Soak the homogenizer and electrophoresis tank with 3% hydrogen peroxide for 20-30 minutes, and then rinse with 0.1% DEPC water. Since the uninactivated DEPC will affect the PCR reaction, i...

Embodiment 2

[0052] Homology Comparison of Coding Sequence of Porcine MAO-A Gene

[0053] Search and obtain the human, bovine, mouse, dog MAO-A gene coding protein sequence on GenBank, and sequence it with the SEQ ID NO.1 sequence obtained by the present invention obtained by cloning and sequencing, and perform sequence alignment on the molecular biology software DNAman source comparison. The comparison results showed that the identity of SEQ ID NO.1 and the CDS sequence of MAO-A gene of human, bovine, mouse, dog and other species was 91.26%, and the identity of amino acid sequence was 92.83%.

[0054] The sequences and symbols involved in the present invention are listed as follows:

[0055] Information on SEQ ID NO.1

[0056] Shanghai Jiao Tong University

[0057] Porcine MAO-A Gene Protein Coding Sequence

[0058]

[0059] 2004-05-17

[0060] 2

[0061] PatentIn version 3.1

[0062] 1

[0063] 1886

[0064] DNA

[0065] pig (sus scrofa)

[0066]

[0067] misc_feature ...

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PUM

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Abstract

Disclosed is a pig monoamine oxidase A protein coding sequence, which includes No.40-1623 nucleotide sequence in SEQ ID NO.1, the monoamine oxidase A protein coded sequence consanguinity with human, rat, cow and dog is 91.26%, it has polypeptide of the amino acid sequence represented by SEQ ID NO.1, or its conservative variation polypeptide, of its active segments, or its active derivative, the monoamine oxidase an amino acid sequence consanguinity with human, rat, cow and dog is 92.83%.

Description

technical field [0001] The invention relates to a protein coding sequence, especially the protein coding sequence of porcine monoamine oxidase A (MAO-A), which is used in the field of genetic engineering. Background technique [0002] Monoamine oxidase A (monoamine oxidase A, MAO-A) is a flavin enzyme bound to the outer mitochondrial membrane, which is responsible for several important monoamine neurotransmitters, including 5-hydroxytryptamine (5-HT), Key enzymes in the oxidative degradation of norepinephrine (NE) and dopamine (DA). Monoamine substances have important functions of nerve activity, blood vessel activity and other life activities, so monoamine oxidase plays a key role in the regulation of normal neuroendocrine system activity. MAO was first discovered by Hare in 1928. In 1970, it was discovered that MAO exists in two forms (Collins et al., 1970), and then the preferred substrates and specific inhibitors of MAO-A and MAO-B were confirmed respectively (Knoll, 1...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12N15/53
Inventor 潘玉春白春艳孟和赵威李婧
Owner SHANGHAI JIAO TONG UNIV
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