Extraction of immunoglobulin

An immunoglobulin and extraction method technology, which is applied in the field of extracting high-purity immunoglobulins, can solve the problems of decreased product quality, long production cycle, low production efficiency, etc., so as to improve production efficiency, improve product quality, and reduce production costs. Effect

Inactive Publication Date: 2005-10-12
宋怀燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, the ammonium sulfate precipitation method is routinely used. This method has high production cost, long production cycle, low production efficiency, and poor purity. In a large amount of extraction process, this method causes poor appearance of the product due to excessive serum hemolysis As a result, the quality of the product is reduced, and it is difficult to remove other impurities other than IgG.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1. Serum volume V---1.0L;

[0016] 2. 1 volume of serum plus 3 volumes of acetate buffer;

[0017] 3. Use NaOH to adjust the pH to 4;

[0018] 4. Add caprylic acid 25ul / ml to dilute the serum;

[0019] Total volume V---ml×25ul / ml=---ul

[0020] 5. Add caprylic acid dropwise with a straw, stir at the same time, and seal the container with tin foil;

[0021] 6. Put the liquid into the centrifuge bottle, balance, and centrifuge at 4000rpm for 30 minutes;

[0022] 7. Put the supernatant into the filter, filter out the floating fat, take out the precipitate from the bottle and store it in the refrigerator;

[0023] 8. Weigh the volume of the solution, put it into an appropriate container, liter (V) ---

[0024] 9. Add PBS 10% by volume;

[0025] Volume V---x 0.10=---Volume V is the amount of PBS added;

[0026] 10. Use NaOH to adjust the pH to 7.2, and measure the amount of NaOH---

[0027] 11. Calculate the total volume of the solution:

[0028] Solution volume + a...

Embodiment 2

[0042] 1. Serum volume V---1.0L;

[0043] 2. 1 volume of serum plus 3 volumes of acetate buffer;

[0044] 3. Use NaOH to adjust the pH to 5;

[0045] 4. Add octanoic acid 40ul / ml to dilute the serum;

[0046] Total volume V---ml×40ul / ml=---ul

[0047] 5. Add caprylic acid drop by drop with a straw, stir at the same time, and seal the container with tin foil;

[0048] 6. Put the liquid into the centrifuge bottle, balance, and centrifuge at 6000rpm for 30 minutes;

[0049] 7. Transfer the supernatant to the inlet filter, filter out the floating fat, and store the precipitate in the refrigerator;

[0050] 8. Weigh the volume of the solution, put it into an appropriate container, liter (V) ---

[0051] 9. Add PBS, 12% by volume;

[0052] Volume V---×0.10=---Volume V is the amount of PBS added;

[0053] 10. Use NaOH to adjust the pH to 7.6, and measure the amount of NaOH---

[0054] 11. Calculate the total volume of the solution:

[0055] Solution volume + added PBS volume...

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Abstract

The invention opened a method of exacting IgG. The method is to put the serum into the ABS then to adjust the pH by the NaOH, next to add the ethyl and PBS, last to add the (NH4)2SO4. The purity of the product is enhanced from 65% to 97%. The method can be used for the serum of rabbit, horse, sheep, chicken and mouse.

Description

technical field [0001] The invention discloses a method for extracting immunoglobulin (IgG), in particular a method for extracting higher-purity immunoglobulin by using animal serum as a raw material under simple conditions. Background technique [0002] Immunoglobulin (Immunoglobulin, Ig) refers to a globulin that has antibody activity or has a chemical structure similar to that of an antibody. Because it was first proved by electrophoresis that the antibody activity in the serum was in the gamma globulin part, the antibodies were collectively referred to as two (gamma) globulins. It was later proved that not all antibodies are in the gamma region; and globulins located in the gamma region do not necessarily have antibody activity. In 1964, the World Health Organization held a special meeting and collectively referred to globulins with antibody activity and antibody-related globulins as immunoglobulins (Ig). Such as myeloma protein, abnormal immunoglobulin in the serum of...

Claims

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Application Information

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IPC IPC(8): C07K1/14C07K16/18
Inventor 宋怀燕
Owner 宋怀燕
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