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Method for preparing nuleus embryo stem cell from somatic cell transplanting technology

A technology of somatic cell nuclear transfer and embryonic stem cells, which is applied in the field of preparation of nuclear transfer embryonic stem cells by somatic cell nuclear transfer technology, can solve problems such as immune rejection, and achieve the effect of accelerating development and solving immune rejection

Inactive Publication Date: 2005-10-19
SOUTHEAST UNIV
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Problems solved by technology

[0006] The purpose of the present invention is to provide a method for preparing nuclear transfer embryonic stem cells by somatic cell nuclear transfer technology that not only solves the ethical problems of embryonic stem cells used in medicine, but also solves the problem of immune rejection

Method used

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Embodiment Construction

[0017] The method for preparing nucleated embryonic stem cells using somatic cell nuclear transfer technology of the present invention is: using a micromanipulator to transplant the neuron nuclei of adult animals into the cytoplasts of the enucleated embryonic stem cells of animal embryos, and reconstitute the nucleated embryos with a new genotype Stem cells, and cultured embryonic stem cells to divide and proliferate to 28. Embryonic stem cells and nerve cells are allogeneic cells, and the prepared cells are nuclear-transferred embryonic stem cells with a nucleated donor cell-nerve cell genotype. Preparation The cell method consists of the following steps:

[0018] 1) Isolate and clone mouse gonadal ridge-derived embryonic stem cells, and establish animal embryonic stem cell lines;

[0019] 2) Isolating nerve cells from animal brain tissue and purifying them;

[0020] 3) Embryonic stem cell enucleation and denucleation from nerve cells;

[0021] 4) Transplant animal nerve c...

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Abstract

The method of preparing nucleus embryo stem cell by means of somatic nucleus transplanting technology is one method of somatic nucleus transplanting process to prepare cell. Under microphone operation instrument, nerve nucleus of adult animal is transplanted into denucleated embryo stem cell cytoplast to form embryo stem cell with completely new genome, and the new embryo stem cell is cultured to divide and proliferate to 28 pieces. Embryo stem cell and nerve cell are homologous cells and the prepared cell is nucleus transplanted embryo stem cell with the genome of donor cell. The present invention solves serial problems in somatic nucleus transplanting and can promote the development of cell engineering and tissue engineering relevant to human embryo stem cell.

Description

1. Technical field [0001] The invention relates to a method for preparing cells by applying somatic cell nuclear transfer technology, and the generated cells can be used for cell engineering such as transplantation therapy. 2. Background technology [0002] Injuries and diseases of the central nervous system have seriously endangered human health for a long time. Although traditional drug therapy and surgical intervention have made limited progress in a certain period of time, there are still gaps in the effect of drugs, timing and location of surgery. insurmountable problem. Therefore, people try to solve this problem with a new treatment method - cell therapy. Cell therapy of the central nervous system refers to the transplantation of autologous, allogeneic or xenogeneic living cells into a specific area in the brain or spinal cord of a patient after processing (changing or not changing their biological characteristics), Thereby treating a certain central nervous system ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0735C12N15/877
CPCC12N15/877C12N5/0606C12N2517/04
Inventor 毛曦储成林浦跃朴林萍华毛钊
Owner SOUTHEAST UNIV
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