Method for preparing nuleus embryo stem cell from somatic cell transplanting technology
A technology of somatic cell nuclear transfer and embryonic stem cells, which is applied in the field of preparation of nuclear transfer embryonic stem cells by somatic cell nuclear transfer technology, can solve problems such as immune rejection, and achieve the effect of accelerating development and solving immune rejection
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[0017] The method for preparing nucleated embryonic stem cells using somatic cell nuclear transfer technology of the present invention is: using a micromanipulator to transplant the neuron nuclei of adult animals into the cytoplasts of the enucleated embryonic stem cells of animal embryos, and reconstitute the nucleated embryos with a new genotype Stem cells, and cultured embryonic stem cells to divide and proliferate to 28. Embryonic stem cells and nerve cells are allogeneic cells, and the prepared cells are nuclear-transferred embryonic stem cells with a nucleated donor cell-nerve cell genotype. Preparation The cell method consists of the following steps:
[0018] 1) Isolate and clone mouse gonadal ridge-derived embryonic stem cells, and establish animal embryonic stem cell lines;
[0019] 2) Isolating nerve cells from animal brain tissue and purifying them;
[0020] 3) Embryonic stem cell enucleation and denucleation from nerve cells;
[0021] 4) Transplant animal nerve c...
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