Micro sensor for detecting hepatic fibrosis based on method of antibody and antigen

A technology of micro-sensors and liver fibrosis, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of high cost, unreliability, cross-contamination detection results, etc., achieve accuracy improvement, reduce measurement error, and facilitate The effect of mass production

Inactive Publication Date: 2005-10-26
FUDAN UNIV
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AI Technical Summary

Problems solved by technology

[0004] The combination of antigen and antibody has a high degree of specificity and sensitivity. Sensors made by traditional methods based on this principle generally require a large sample volume (serum), and the cost of one-time use is too high. Recycled use may cause cross-contamination. Test results are unreliable

Method used

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  • Micro sensor for detecting hepatic fibrosis based on method of antibody and antigen
  • Micro sensor for detecting hepatic fibrosis based on method of antibody and antigen
  • Micro sensor for detecting hepatic fibrosis based on method of antibody and antigen

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Embodiment Construction

[0020] The present invention is further described by the following examples.

[0021] The micro-sensor is made by the lift-off method after evaporation (the gold electrode is also used for the counter electrode). It can also be prepared by sputtering instead of evaporation, and etching instead of lift-off.

[0022] a. Wash the silicon wafers with No. 1 and No. 2 liquids, rinse with deionized water, and dry.

[0023] b. Thermally grown 300nm SiO 2 .

[0024] c. Photoetch the required working electrode and counter electrode patterns, such as figure 1 with figure 2 Shown. There are six working electrodes 3 arranged symmetrically on both sides, with a diameter of 1 mm and a spacing of 200 microns. The spacing between the working electrode 3 and the reference electrode 1 is 200 microns, and the spacing between the reference electrode 1 and the counter electrode 2 is also 200 microns.

[0025] d. Evaporate the titanium layer to a thickness of about 20nm, and then evaporate gold to a t...

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Abstract

This invention belongs to the field of the silica micro-machine and the electrical chemistry, specific about a kind of micro sensor on the base of antibody and antigen method to detect the fibration of the liver. It is made up of the working electrode, reference electrode, pairing electrode and the base board. Among these, the working poles have the shape of circle or like a circle and they range symmetrically. And the reference electrode is like the letter 'U', set between the working electrode. Besides, the pair pole looks like a trip, set between the reference poles. The base board is made up of the composite silicon packing block and the SiO2 layer. There are 4-8 working electrode and the electrode and the SiO2 layer are bonded with the Ti tack coat, and each electrode can be made with the simple film technology of the IC technology. In a word, the invention has small micro-sensors, costs little and has high authenticity. It can detect out the concentration of the antigen in the serum quantitatively and meanwhile measure several data of the serum, and the quantity of the serum is small.

Description

Technical field [0001] The invention belongs to the technical field of silicon micromachines and electrochemistry, and specifically relates to a silicon micromachined microsensor for detecting liver fibrosis based on an antibody antigen method. Background technique [0002] Liver fibrosis refers to the pathological process of abnormal proliferation of fibrous connective tissue in the liver when hepatocytes undergo necrosis and inflammatory stimulation. It is a chronic and gradual process. The vast majority of chronic liver diseases have liver fibrosis, and 25%-40% of them eventually develop into cirrhosis and even liver cancer. Therefore, liver fibrosis is not only the most important pathological feature of chronic liver disease, but also an important reason for the further development and deterioration of chronic hepatitis and cirrhosis. Therefore, early detection of liver fibrosis is of great significance to patients with liver disease. [0003] The diagnostic methods currently...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48
Inventor 黄洪湖周嘉史绵红黄宜平孔继烈
Owner FUDAN UNIV
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