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Vaccines containing viruses involved in avian malabsorption syndrome and methods of administration therefor

A malabsorption syndrome technology, applied in the field of vaccines against poultry malabsorption syndrome-related diseases, can solve the problem that the diagnosis has not yet been confirmed

Inactive Publication Date: 2005-11-23
WYETH LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease occurs in various parts of the world, but the diagnosis of the disease in susceptible chickens has not been confirmed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Materials and Methods

[0054] Inoculum was prepared from intestines (including duodenum and cecum) samples taken from 10 chicks from a site showing clinical symptoms of MAS disorders. The gut samples were stored at -20°C. 100 grams of intestines were homogenized in 100 ml PBS using a laboratory homogenizer. This tissue homogenate (50% w / v) was inoculated to 1-day-old broiler chickens.

[0055] Eighty one-day-old broiler chicks were obtained from a commercial hatchery. These chickens were divided into 2 groups, 40 in each group, and raised in different isolation areas. Paper was laid on the ground of the isolation area to observe the feces. 40 chicks (group 2) were inoculated with 0.5 ml intestinal tissue homogenate by inserting a cannula into the crop. The remaining 40 chicks (group 1) were not inoculated and served as the uninfected control group. The chicks were fed unlimited commercial broiler feed and had free access to water. Prior to this, they had not bee...

Embodiment 2

[0081] The purpose of this study was to investigate whether one infectious agent or multiple infectious agents caused MAS to spread through the peripheral blood. This was done by inoculating 1-day-old broiler chick crops with homogenates of pancreas, yolk sac and liver tissue from infected chicks.

[0082] Twenty 1-day-old broiler chickens (group 1) were inoculated with 0.5 ml of intestinal tissue homogenate (stored at -70°C) by inserting a cannula into their crops, and then housed on the ground covered with sawdust. The chicks were sacrificed on day 4 after inoculation. Carefully remove the liver, pancreas, yolk sac, and intestine to avoid contamination with intestinal material. Intestines were stored at -70°C. The liver, pancreas and yolk sac were homogenized. These tissue homogenates were used to inoculate three new groups (groups 2, 3 and 4) of 20 1-day-old broiler chicks, each of which was inoculated by inserting a cannula into the crop. The groups were housed on sawd...

Embodiment 3

[0122] 50 1-day-old broiler chickens were divided into 5 groups, 10 in each group, and were inoculated as follows by inserting the intubation method into the crop: the first group (infection control) was inoculated with intestinal tissue homogenate; the second group (reovirus) Vaccination 10 6.7 TCID 50 Reovirus; group 3 (adenovirus) inoculated 10 8.2 TCID 50 Adenovirus; group 4 (adenovirus and reovirus) inoculated 10 6.7 TCID 50 Reovirus and 10 8.2 TCID 50 Combination of adenoviruses. Group 5 (uninfected controls) was not vaccinated. The chickens of each group were kept in stainless steel cages in the isolated animal room, with barbed wire and feces collection devices on the bottom of the cages. On days 14 and 22 after inoculation, the chicks of each group were weighed and sacrificed.

[0123] The changes in the longitudinal sections of the two proximal tibial epiphyseal cartilage plates of each bird were observed, and the bone disease was evaluated visually. Intest...

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Abstract

The causative agent(s) of Avian Malabsorption Syndrome (MAS) are isolated and used to prepare vaccines for use in the prevention of diseases resultant therefrom. The vaccines contain at least two avian viruses - the reovirus and adenovirus - and optionally include another virus which inflicts poultry. The viruses may be live, attenuated live, or inactivated when incorporated into the vaccine. The vaccine itself may be administered in ovo, to new-born or growing chicks, or to adult fowl.

Description

field of invention [0001] The present invention relates to a vaccine against poultry diseases, more particularly, the present invention relates to a vaccine against poultry malabsorption syndrome (MAS) related diseases and a method of administration to poultry. Background of the invention [0002] Malabsorption Syndrome (MAS) is a disease of mid-chicken poultry, especially affecting broiler or broiler type chickens most commonly. This syndrome has been reported in the Netherlands (Kouwenhoven et al.; 1978) and is called "growth retardation and dwarfism syndrome" in chickens. The disease is known by various names around the world, including infectious dwarf syndrome, pale fowl syndrome, helicopter sickness, infectious gastritis, brittle bone disease, and necrosis of the femoral head. [0003] Kouwenhoven et al. (Avian Pathology 17, 879-892, 1988) further defined MAS according to the following five criteria: [0004] 1) Abnormal growth in 1-day-old chickens up to 3 weeks aft...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61KA61K39/15A61K39/235A61K39/295
CPCA61K39/235A61K2039/545A61K2039/5254A61K2039/552A61K2039/54A61K2039/5252A61K39/15A61K39/295A61K2039/55A61K39/12A61K2039/70A61P31/12A61P31/20C12N2710/10234C12N2720/12334
Inventor M·H·韦尔托门F·G·戴夫拉尔J·J·罗韦伦斯
Owner WYETH LLC
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