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Inhibiting biofilm formation by thermophilic microbes in paper and board machines

A technology for cardboard machines and paper machines, which is applied in the field of quantitatively equipping anti-biofilm reagents, and can solve problems such as affecting paper quality, web breakage, and clogging of filters.

Inactive Publication Date: 2005-12-28
KEMIRA OY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When spontaneously detached from the surface, biofilm deposits can clog filters, cause web breakage, and affect paper quality, for example by forming holes or stains

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] A total of 110 pure substances were studied. Table 1 shows that the 9 pure substances had a strong anti-biofilm effect on more than one of the adherent bacterial strains (>50% reduction in biofilm on more than one of the tested strains).

[0057] The 3 pure substances (lauryl gallate, octyl gallate and nordihydroguaiaretic acid) have broad-spectrum anti-biofilm effects as they are present in 250 μmol L -1 The concentration of reduced biofilm formation for all 4 different adherent bacteria. Gallic acid esters, especially lauryl gallate in 25 μmol L -1 content is also effective. The molecular weight of lauryl gallate is 338.45g mol -1 , that is, the substance in 8.5mg L -1 (=8.5 ppm) content has broad-spectrum anti-biofilm efficacy. Octyl gallate and lauryl gallate reduced the adhesion of biofilm bacteria to surfaces in nutrient-poor solutions by up to more than 90% (250 μΜ for Deinococcus geothermalis and Burkholderia cepacia).

[0058] Many pure substances were foun...

Embodiment 2

[0062] The best anti-biofilm agent of Example 1 was further investigated by including several strains in the experiment and testing without SDS washes.

[0063] Included were the adherent bacterial strains E-lvk-R2A-1 and E-jv-CTYE3 isolated from paper machines and not yet identified, and Aspergillus fumigatus G3.1. The reference substance used was the commonly used biocide Fennosan M9, the active ingredient of which was methylene dithiocyanate (9%). The results are shown in Table 2.

[0064] It was also confirmed that gallic acid esters are also effective against new adherent bacteria. They were also effective without SDS washing, leading to the conclusion that the mechanism of effect of these substances includes inhibition of biofilm formation. Even at a level of 25 [mu]M, lauryl and octyl gallate were active against most of the microorganisms tested. They are also effective against difficult-to-control Burkholderia cepacia and Thermomonas biofilms. Surprisingly, low lev...

Embodiment 3

[0073] Also studied in paper machine water culture (white water, supplemented with 1 g / L of starch and 300 mg / L of yeast extract, sterilized, 250 μl / cup, pH 7, inoculated with 2%, grown for 48 hours, 45 °C, 160 rpm) The anti-biofilm effect of the most effective pure substance of Example 1 was determined. The results are shown in Table 3. Octyl gallate and lauryl gallate reduced the adhesion of biofilm microorganisms to surfaces by up to more than 90% in sterile white water (for Meiothermus silvanus and adherent bacteria E-jv-CTYE 3 at a level of 25 μΜ). Longer incubation times are required for adherent bacteria to form biofilms in paper machine water. The effect of both pure substance and M9 was small, probably due to the longer incubation time. In a paper machine environment, this problem does not exist because the active ingredient is added to the process at regular intervals.

[0074] Table 3: Anti-biofilm effect of the best pure substance (in DMSO) in white water, witho...

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PUM

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Abstract

The present invention relates to a method for inhibiting the formation of biofilms on the surfaces of paper and board machines by thermophilic adherent microorganisms of paper and board machines, and / or for removing such biofilms from said surfaces, by applying Add at least one pure substance isolated from plants, or at least one plant extract or a mixture thereof, to the circulating water of the machine at a concentration effective against thermophilic adherent microorganisms. The present invention also relates to a method of determining whether an anti-biofilm agent needs to be added to a paper and board production process and a kit suitable for use in this method.

Description

technical field [0001] The present invention relates to a method for inhibiting biofilms on the surfaces of paper and board machines, which biofilms are formed by thermophilic bacteria and / or molds and affect processing. The invention also relates to a method of determining the need for dosing an anti-biofilm agent into a process for the manufacture of paper and board and a kit suitable for use in this method. Background technique [0002] The environment of the paper machine is conducive to the growth of various microorganisms. The water of the paper machine provides the microorganisms with the nutrients they need, a suitable pH (4 to 9) and temperature (45 to 60°C). Microorganisms enter production along with raw materials such as fibres, chemicals and water. Free-swimming microorganisms are not as detrimental to production as microorganisms that adhere to paper machine surfaces and form biofilms. Washing biofilm off paper machine surfaces is difficult and often requires...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/34A01N37/40A01N65/08A01N65/12A01N65/22A01P1/00A61L2/18C02F1/50D21H17/02D21H17/06D21H21/02
CPCA01N37/40A61L2/18C02F1/50C02F2103/023C02F2103/28D21H17/02D21H17/06D21H21/02A01N65/08A01N65/22A01N65/34
Inventor M·考拉里M·萨尔金诺亚-萨罗恩H·拉缇凯恩P·塔梅拉P·沃勒拉P·瓦坦恩T·J·哈图恩
Owner KEMIRA OY
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