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Para-gene sequence for exogenous insertion vector of corn strain MON863

A MON863, exogenous insertion technology, applied in genetic engineering, plant gene improvement, introduction of foreign genetic material using vectors, etc., can solve problems such as reports, articles and patents without adjacent gene sequence analysis

Inactive Publication Date: 2006-03-01
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the analysis of existing patents and documents, it is found that there are no articles or patent reports on the sequence analysis of the adjacent gene sequence of the exogenous insertion vector of the MON863 maize line

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Cloning of Adjacent Sequences of Foreign Insertion Vector in MON863 Maize

[0016] 1. Experimental materials

[0017] 1. Plant material

[0018] Genetically improved corn: MON863 corn, Mon810 corn, Bt11 corn, Bt176 corn, TC1507 corn, GA21 corn, etc.

[0019] conventional corn

[0020] 2. Enzymes and reagents

[0021] The Plant Genome DNA Extraction Kit is a food DNA extraction kit jointly developed by Shanghai Academy of Agricultural Sciences and Shanghai Entry-Exit Inspection and Quarantine Bureau.

[0022] dNTPs, Taq DNA polymerase and its buffer, and DL2000 Marker were purchased from Dalian Bao Biological Engineering Co., Ltd. Random primers, TaqMan probes and primers were synthesized by Shanghai Boya Bioengineering Co., Ltd.

[0023] Other biochemical reagents are imported subpackages or domestic analytical pure.

[0024] 3. Experimental equipment

[0025] DY-501 Nucleic Acid Electrophoresis Apparatus (Shanghai Precision Scientific Instrument Co., Ltd.)

[0...

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PUM

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Abstract

This invention relates to the side gene sequence of MON863 maize exogenous inserted carrier. It includes: partial nucleotide sequences which codes maize NADH dehydrogenase subunit 1and 2. This nucleotide sequence is totally identical with either 1-253 nucleotide sequence in the SEQ ID NO.1 or 138-411 nucleotide sequence in the SEQ ID NO.2. The partial sequences of 35S promoter, which code the tobacco mosaic virus, come from exogenous inserted carrier PV-ZMIR13 and have the same nucleotide sequence with 254-456 nucleotide sequence in the SEQ ID NO.1. The partial sequences, which code the wheat tahsp 17 3í» terminator, come from exogenous inserted carrier PV-ZMIR13 and have the same nucleotide sequence with 1-137 nucleotide sequence in the SEQ ID NO.2.

Description

technical field [0001] The present invention relates to a gene sequence in the field of biotechnology. Specifically, it relates to the adjacent gene sequence of a foreign insertion vector of a maize line MON863. Background technique [0002] With the wide application of plant genetic transformation technology in basic research and applied research, a variety of transgenic plants have been produced. The adjacent gene sequence of the exogenous insertion vector of the transgenic plant is an important biological characteristic of the transgenic line. Therefore, the adjacent gene sequence of the exogenous insertion vector is an important experimental basis for establishing a specific detection method for the transgenic plant line. [0003] At present, some patents and documents have analyzed the adjacent gene sequence of the exogenous insertion vector of transgenic plants. For example, Holck A et al. analyzed the adjacent gene sequence of MON810 maize by TAIL-PCR in 2002, which ...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/53C12N15/82C12Q1/68
Inventor 张大兵杨立桃潘爱虎梁婉琪尹长松许诵辞章可为武爱波
Owner SHANGHAI JIAO TONG UNIV
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