36 results about "Tobacco mosaic virus TMV" patented technology

The invention discloses a synthetic method and bioactivity of beta-amino acid esters which have optical activity and contain benzothiazole groups. The beta-amino acid esters which have optical activity and contain the benzothiazole groups have the structure shown in the general formula (I), and in the formula, R1 is a group of hydrogen, p-chloro, o-chloro, p-fluoro, o-fluoro, p-methyl, o-methoxy and the like; R2 is a group of 4-methyl, 6-methoxy and the like; and R3 is a group of methyl, ethyl, propyl, isopropyl and the like. The invention introduces Mannich reaction which uses organic catalyst of cinchona alkaloid thiourea to catalyze benzothiazole imine and malonate, the product is obtained by one-step synthesis, and the reaction yield and the selectivity to enantiomer are both high. Compounds c, d and e in the inveniton have high therapeutic and passivation inhibiting effects towards tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV) and show good anti-plant virus activity.

Protein scaffolds from tobacco mosaic virus coat protein modified to incorporate polyhistidine can bind to a metal or a dye while having improved self-assembly characteristics. The scaffold can take the form of tubes or disks, and can further be formed into dual plasmonic ring resonators. Such self-assembled structures provide useful optical properties.

The invention relates to a virus-induced gene silencing system in particular using tobacco mosaic virus TMV as a vector. In the system, a DNA segment of a target gene is inserted between a termination codon and a 3' untranslated region of coat protein CP of the tobacco mosaic virus TMV; a polyclonal restricted endo enzyme cutting point is introduced between the termination codon and the 3' untranslated region of the coat protein CP of the tobacco mosaic virus TMV and is used for the insertion of the DNA segment of the target gene; and a section of a basic group sequence on the bottom end of CP3' is introduced in the front of the 3' untranslated region so as to strengthen the stability of the silencing vector. The DNA segment of the target gene is not more than 1 kb. The gene silencing system is utilized to carry out silencing on the target gene and is economic, feasible, convenient and effective.

The present invention relates to a phenanthroindolizidine alkaloid glycosylation product, a 6-site derivatization product, and preparation methods and plant virus resistance activities of the phenanthroindolizidine alkaloid glycosylation product and the 6-site derivatization product, wherein various groups in the formula are defined in an instruction. According to the present invention, the phenanthroindolizidine alkaloid glycosylation product has characteristics of good light stability, good thermal stability, good water solubility and excellent plant virus resistance, and the glycosylation product and 6-site derivatization product can well inhibit tobacco mosaic virus (TMV). The formula I is as the follow.

The invention discloses a poly-substituted miazines compound having anti-plant virus activity, a preparation method and application thereof. The structure of the poly-substituted miazines compound is shown in formula (I). Experiments prove that the poly-substituted miazines compound has remarkable effect of growth inhibition to the tobacco mosaic virus, so that the compound can be made into antiviral agents with different concentrations and types according to the actual production and be applied to agricultural industry.

The invention discloses a preparation method for pyrazolyloxadiazole thioether compounds and application of the pyrazolyloxadiazole thioether compounds to resisting of the activity of a tobacco mosaic virus (TMV). The compounds have a structure as shown in a general formula (I) which is described in the specification. According to the invention, on the basis of a 5-amino-1-(3-chloro-2-pyridyl)-4-(5-methylmercapto-1,3,4-oxadiazol-2-yl)pyrazol structure, a series of the pyrazolyloxadiazole thioether compounds with a novel structure are synthesized through substitution of an amino group at position 5. Results of testing of the protection and treatment activity of the compounds to the TMV show that the compounds have good treatment activity to viral diseases caused by the TMV and lay an important scientific foundation for research, development and creation of novel pesticides.

The present invention relates to a triazole compound. Said invention provides its general formula. Its preparation method includes the following steps: using 5-triazo-1,2,4 triazol-3-acyl methyl ester and propiolic alcohol ester acetate, p-amyl phenylacetylene, syclohexanol alkyne, cyclohexene alkyne or methoxyphenyl acetylene, and making them be reacted in the tetrahydrofuran or mixed solvent of tetrahydrofuran and water at 10-100 deg.C under the action of Cu(I) catalyst so as to obtain the required triazole compound. Said triazole compound has the activity for directly inhibiting plant virus, in particular inhibiting tobacco mosaic virus.

The invention describes compositions and methods of use in which an infectious modified Tobacco Mosaic Virus (TMV) virion comprising a coat protein (CP) or a movement protein (MP) gene is replaced with a nuclear inclusion protease (NIa) expression cassette for the expression of a heterologous peptide in a tobacco mosaic virus (TMV) host plant.

The invention discloses a microsensor-controllable array preparation method using tobacco mosaic virus as a template. According to the method, a phage display technology is utilized to obtain a polypeptide molecule that binds to a specific functional nanoparticle; and a gene sequence corresponding to the polypeptide molecule is obtained by gene sequencing. Subsequently, the tobacco mosaic virus isgenetically modified by a molecular biological means, and the polypeptide molecule that binds to the specific functional nanoparticle is adsorbed on coat protein to bind to the specific functional nanoparticle to obtain sensitive units; and at the same time, cysteine (Cys) is introduced into substrate surface protein, by using a binding mechanism of the cysteine and gold (Au) nanoparticles, arraydistribution of the sensitive units of a sensor can be achieved. The method using the tobacco mosaic virus as the template to realize sensor controllable array can greatly reduce an array unit size,improves the resolution and sensitivity of the sensor, and can be applied to array design and preparation of pressure sensors and gas sensors.

The invention discloses a method for preparing a tobacco mosaic virus capsid protein capsule. The method comprises the following steps: preparing a tobacco mosaic virus capsid protein solution, mixing the tobacco mosaic virus capsid protein solution with an oil phase to form an emulsion, adjusting the pH value of the system to control the tobacco mosaic virus capsid protein to form a rod-shaped combined structure, and cross-linking the rod-shaped tobacco mosaic virus capsid protein at an oil-water boundary by using glutaraldehyde as a cross-linking agent, thereby obtaining the tobacco mosaic virus capsid protein capsule. The method for preparing the tobacco mosaic virus capsid protein, which is disclosed by the invention, is simple in step, short in preparation cycle, high in surface wrapping rate of prepared protein capsules, and solid and uniform in capsule structure.

Protein scaffolds from tobacco mosaic virus coat protein modified to incorporate polyhistidine can bind to a metal or a dye while having improved self-assembly characteristics. The scaffold can take the form of tubes or disks, and can further be formed into dual plasmonic ring resonators. Such self-assembled structures provide useful optical properties.

The invention provides chirality oliopeptide phosphonate thiourea derivatives and application and a preparation method thereof. A series of chirality oliopeptide phosphonate thiourea derivatives of novel structures are designed and synthesized. Each derivative comprises an amino acid skeleton, a thiourea-based functional group and an amino phosphonate component, and the molecular structure is provided with multiple chirality centers. Meanwhile, the compounds have a good inhibiting effect on plant viruses, and are suitable for preparing plant virus resistant agents and particularly suitable for new pesticide creation and research for tobacco mosaic viruses (TMV). The preparation method is good in reliability, good symmetry selectivity and high yield are achieved, and the material basis is provided for follow-up research. The material source is simple, and the material is easy to obtain, low in cost and good in use effect.

The invention discloses a preparation method for 1-phenyl-5-amino-4-pyrazole bi-oxadiazole thioether compounds and an application in tobacco mosaic virus resistant activity. The compounds have structures represented by a general formula (I) in the description. The method provided by the invention is based on a 5-amino-1-(3-chloro-2-pyridyl)-4-(5-methylthio-1,3,4-oxadiazole-2-yl)-pyrazole structureand uses ''phenyl'' to replace ''3-chloro-2-pyridyl'' on the 1-position of a pyrazole ring to synthesize a series of the 1-phenyl-5-amino-4-pyrazole bi-oxadiazole thioether compounds, and experimentsof the compounds in vivo show that the compounds have good curative protective and inactivating activity on virus diseases caused by the tobacco mosaic virus (TMV), and the compounds show significanteffects on passivation effects compared with compounds reported in the earlier work of a research group; and the compounds provide an important scientific basis for the research, development, creation of novel pesticides.

The invention relates to a Kealiinine derivative II, a preparation method thereof and an application of the derivative II in resisting plant viruses and germs. The Kealiinine derivative II shows especially excellent plant virus resisting activity, can well inhibit tobacco mosaic viruses (TMV), and also shows certain plant pathogenic germ resisting activity.

The invention discloses Disporum cantoniense polysaccharide extracted by an ultrasound-assisted enzymatic method and an extraction method and application thereof. The extraction method comprises taking 1 part by mass of Disporum cantoniense powder which is sieved by a 60-mesh sieve, soaking the Disporum cantoniense powder in 15-50 parts by mass of distilled water, adjusting the pH value to 3.5-7.0, standing the solution at the temperature of 30-70 DEG C for 15-30 minutes, then adding an enzyme which accounts for 0.1-10% of the mass of the dry powder of the Disporum cantoniense into the solution, carrying out enzymolysis at 30-70 DEG C for 0.5-3 hours to obtain a mixture, carrying out ultrasonic extraction at the temperature of 30-70 DEG C for 10-60 minutes, carrying out enzyme deactivationtreatment at the temperature of 80-90 DEG C for 5-20 minutes, carrying out cooling at the room temperature, centrifuging the mixture at the speed of 3000 to 6000 rpm for 10 to 30 minutes, concentrating the supernatant to 1 / 2-1 / 5 volume by using a rotary evaporator, then adding absolute ethyl alcohol of which the volume is 4-5 times that of the concentrated solution into the supernatant, standingthe solution at 4 DEG C for 2-8 hours, centrifuging the solution at the speed of 5000-6000 rpm for 10-20 minutes, washing the solution by using absolute ethyl alcohol, and freeze-drying the solution to obtain the product. The Disporum cantoniense polysaccharide has toxicity to tumor cells, has good inhibitory activity to tobacco mosaic viruses and cucumber mosaic viruses, and is high in extractionrate, short in extraction time, energy-saving and environment-friendly.

The present invention provides a chloroisobromine cyanuric acid mixing preparation capable of preventing and controlling tobacco mosaic virus, wherein the active components comprise, by weight, 10-40 parts of lemongrass essential oil, 20-50 parts of a fragrant eupatorium herb alcohol extract, 5-20 parts of camellia oil, and 10-40 parts of chloroisobromine cyanuric acid. According to the present invention, the active components comprise the lemongrass essential oil, the fragrant eupatorium herb alcohol extract, the camellia oil and the low environment toxicity chloroisobromine cyanuric acid, such that the obtained chloroisobromine cyanuric acid mixing preparation has advantages of low toxicity, no pollution and no residue, is the environmentally friendly product, can avoid the problems such as environmental pollution and pesticide residues in agricultural production, and can provide significant passivation, prevention and treatment effects for tobacco mosaic virus.

The present invention relates to a triazole compound. Said invention provides its general formula. Its preparation method includes the following steps: using 5-triazo-1,2,4 triazol-3-acyl methyl ester and propiolic alcohol ester acetate, p-amyl phenylacetylene, syclohexanol alkyne, cyclohexene alkyne or methoxyphenyl acetylene, and making them be reacted in the tetrahydrofuran or mixed solvent of tetrahydrofuran and water at 10-100 deg.C under the action of Cu(I) catalyst so as to obtain the required triazole compound. Said triazole compound has the activity for directly inhibiting plant virus, in particular inhibiting tobacco mosaic virus.

The invention relates to the application of nortopsentin alkaloids and derivatives I thereof to resisting of plant viruses and pathogens and killing of insects. The nortopsentin alkaloids and the derivatives I thereof in the invention show particularly excellent activity against plant viruses, can effectively inhibit tobacco mosaic viruses (TMV), and also present excellent activity against plant pathogens and insecticidal activity. Substituents in a general formula as shown in the specification are defined in the specification.

The invention discloses primers and kit for detecting tobacco mosaic viruses RT-LAMP. The kit comprises 0.5 milliliters of a RT-LAMP reaction solution buffer, 100 micro-liters of the primers, 60 micro-liters of a fluorescent dye, 60 micro-liters of an enzyme solution, 60 micro-liters of a positive control and 100 milliliters of deionized water. The primers for detecting tobacco mosaic viruses RT-LAMP solve the problems of the existing primers. The kit contains the primers and has the characteristics of rapid detection, high detection sensitivity, simple operation and direct reading of the result.

The invention relates to a meridianin derivative I as well as a preparation method and application thereof in prevention and treatment of plant virus and bacterial diseases. The meridianin derivativeI disclosed by the invention shows especially excellent plant virus resisting activity, can well inhibit tobacco mosaic virus (TMV), and also shows certain plant pathogenic bacterium resisting activity.

The invention discloses application of tobacco mosaic virus in stimulation of polarization of macrophages into M1 type macrophages. In the application, the tobacco mosaic virus can promote polarization of macrophages to M1 type, so that the macrophages have a killing effect, and a treatment scheme is provided for tumor immunotherapy.

The invention discloses a sesquiterpenoid compound with a novel structure. The compound is names as (2R,3S)-2,3-dihydro-3-hydroxyl-6-isopropyl-2,4,7-trimethylindene-1-one, the molecular formula is C15H20O2, and the structure is represented in the description. The invention also discloses a preparation method and applications of the compound. The results of activity tests show that the compound has a good inhibiting effect on tobacco mosaic virus, and can be used as a lead compound for the research on drugs for preventing tobacco mosaic virus.

The invention discloses a method for killing tobacco mosaic virus. The method particularly comprises the following steps: (1) selecting fresh tobacco leaves seriously infected with mosaic virus, arranging the selected tobacco leaves in an oven, drying at the temperature of 60 to 80 DEG C until the moisture content is 10% to 15% and then crushing the dried tobacco leaves to be 20 to 40 meshes, crushing tobacco stems, tobacco stalks and other materials to be 0.5 to 3cm to obtain tobacco powder; (2) arranging the tobacco powder in a QB series supersonic ejection type steam explosion machine, injecting steam so as to enable the pressure in the steam explosion machine to reach 0.52 to 2.70MPa, maintaining the pressure for 90 to 150s and then automatically discharging, wherein the mosaic virus killing rate of tobacco powder reaches 90% to 100%. The method provided by the invention has the advantages of easiness and convenience in operation, high efficiency and low energy consumption.

The invention discloses a diphenol compound (I) in tobacco rhizome and a preparation method and application thereof. Tobacco whole plant is crushed, and then ultrasonically extracted with methanol, the extracted liquid is merged, filtered, and concentrated under vacuum into extract; the extract is primarily separated by silica gel column chromatography; a chloroform-acetone solution with the volume ratio of 1:0 to 1:2 is used for gradient elution; eluent 7:3 part is further separated and purified by high pressure liquid chromatography to obtain the required diphenol compound. The experiment proves that the biphenyl compound has good inhibition effect on tobacco mosaic virus, and can be used as a lead compound against the tobacco mosaic virus.

The invention relates to Topsentin derivatives I as well as preparation and application thereof in resistance to plant viruses and germs. The Topsentin derivatives I show especially excellent anti-plant virus activity, can well inhibit tobacco mosaic viruses (TMV), and also show good anti-plant germ activity, and details of referred content of substituent groups in a general formula see the specification. (The general formula is described in the specification).

The invention relates to a 9-substituted sylphenine derivative and its preparation and application in anti-plant virus. The meaning of R in the general formula can be found in the description. The invention provides a novel anti-tobacco mosaic virus structure, which is advantageous for industrialization, simple in synthesis and the like. The 9-substituted seraphine derivatives exhibit excellent anti-plant virus activity, and can well inhibit tobacco mosaic virus (TMV).

The invention discloses a combined heptatomic ring biphenyl compound (I) and a preparation method and application thereof. Tobacco whole plant as a raw material is extracted by high concentration methanol or high concentration acetone / water or high concentration ethanol / water, the extracted liquid is merged, filtered, and concentrated under vacuum into extract; the extract is loaded into a column by silica gel dry method for silica gel column chromatography; a chloroform-acetone solution is used for gradient elution; eluent 9:1 part is further separated and purified by high pressure liquid chromatography to obtain the required biphenyl compound. The activity test proves that the biphenyl compound has good inhibition effect on tobacco mosaic virus, and the biphenyl compound has simple structure and good compound activity, and can be used as a lead compound against the tobacco mosaic virus.

The invention discloses a furan-carboxylic acid compound as well as a preparation method and application thereof; the furan-carboxylic acid compound is obtained by being separated from flue-cured tobacco leaves, has a molecular formula of C14H14O5, is named as 6-(3'-hydroxy-4'-methoxy-5'-methyl phenyl)-3-methyl-furan-2-carboxylic acid in English, and has a structure described in the specification. The preparation method of the furan-carboxylic acid compound takes the flue-cured tobacco leaves as raw materials and comprises the steps such as extract extraction, silica gel column chromatography and high-pressure liquid chromatography separation; the preparation method concretely comprises the following steps: crushing a sample, extracting, filtering, concentrating, carrying out chromatography, eluting, and purifying the 1: 1 part of eluant by the high-pressure liquid chromatography separation to obtain the furan-carboxylic acid compound. An activity test proves that the furan-carboxylic acid compound has a good inhibiting effect for tobacco mosaic virus. The furan-carboxylic acid compound is simple in structure and better in activity, and can be used as a pilot compound of a tobacco mosaic virus-resistant medicine.

The invention discloses a compound of resisting plant viruses: a pentadienone compound containing quinazolinone aryloxy and a preparation method and biological activity. The invention introduces a series of novel pentadienone derivatives containing quinazolinone aryloxy which are synthesized by six steps by taking substituted o-aminobenzoic acid, formamide, 35% formalin, 1, 4-dioxane, thionyl chloride, hydroxyl benzaldehyde, acetone, sodium hydroxide, hydrochloric acid, potassium carbonate, potassium iodide, substituted aromatic aldehyde, substituted heterocyclic aldehyde and the like as raw materials. The compound disclosed by the invention further has higher treating, protecting and passivating and inhibiting effects to cucumber mosaic virus (CMV), tobacco mosaic virus (TMV), southern rice black streaked dwarf virus (SRBSDV) and rice stripe virus (RSV), shows higher anti-plant virus activity, and can be used for preparing anti-plant virus pesticides.