Disporum cantoniense polysaccharide extracted by ultrasound-assisted enzymatic method and extraction method and application thereof

An auxiliary enzyme and ultrasonic technology, which is applied in the application field of ginseng polysaccharide on tumor cytotoxicity and antiviral field, can solve the problems such as tumor cytotoxicity, anti-plant virus activity, high extraction cost and long extraction time that have not yet been seen. , to achieve good therapeutic and protective activities, simplify the purification process, and reduce the extraction temperature.

Active Publication Date: 2020-04-03
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method provides a scientific basis and an experimental basis for the development of polysaccharides of ginseng polysaccharides, but there are still the following deficiencies in process promotion and industrial production: ①The traditional water extraction method has a low yield, and the extraction time is long, and the energy consumption is large. The extraction cost is high; ② a large amount of organic solvents are used in the extraction and purification process, which brings environmental pollution
However, there are no reports on the cytotoxicity and anti-plant virus activity of Enzyme Treatment, Ultrasonic Treatment or Enzymatic Hydrolysis and Ultrasound-assisted Extraction of Panax ginseng polysaccharides.

Method used

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  • Disporum cantoniense polysaccharide extracted by ultrasound-assisted enzymatic method and extraction method and application thereof
  • Disporum cantoniense polysaccharide extracted by ultrasound-assisted enzymatic method and extraction method and application thereof
  • Disporum cantoniense polysaccharide extracted by ultrasound-assisted enzymatic method and extraction method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0022] A method for ultrasonic-assisted enzymatic extraction of polysaccharides from ginseng, comprising the following steps:

[0023] Weigh 5g of the ginseng powder passed through a 60-mesh sieve, add 150mL of distilled water with a pH value of 7.0, keep it at 60°C for 20min, then add 0.5g of α-amylase to the soaked sample, and incubate at 70°C Enzyme hydrolysis for 3 hours, the mixture was placed in an ultrasonic instrument, ultrasonically extracted for 60 minutes in a water bath at 70°C and an ultrasonic power of 400W, and then the mixture was placed at 90°C for 20 minutes to deactivate the enzyme preparation, and then cooled to Centrifuge at room temperature at 3000rpm for 10min, transfer the supernatant, use a rotary evaporator to concentrate the obtained extract to 1 / 5 volume, then add absolute ethanol 5 times the volume of the concentrate, and stand at 4°C for 8h to precipitate polysaccharides. Centrifuge at 6000rpm for 20min, wash again with absolute ethanol, centrifug...

Embodiment 2

[0028] A method for ultrasonic-assisted enzymatic extraction of polysaccharides from ginseng, comprising the following steps:

[0029] Weigh 5g of the ginseng powder passed through a 60-mesh sieve, add 150mL of distilled water with a pH value of 7.0, keep it at 60°C for 20min, then add 0.1g of β-amylase to the soaked sample, and incubate at 60°C Enzyme hydrolysis at low temperature for 2 hours, the mixture was placed in an ultrasonic instrument, ultrasonically extracted for 15 minutes in a 60°C water bath and an ultrasonic power of 300W, and then the mixture was placed at 95°C to inactivate the enzyme for 10 minutes to denature and inactivate the enzyme preparation, and then cooled to Centrifuge at 3500rpm for 10min at room temperature, transfer the supernatant, concentrate the obtained extract to 1 / 2 volume with a rotary evaporator, then add absolute ethanol 4 times the volume of the concentrated solution, and stand at 4°C for 4h to precipitate polysaccharides. Centrifuge at ...

Embodiment 3

[0032] A method for ultrasonic-assisted enzymatic extraction of polysaccharides from ginseng, comprising the following steps:

[0033]Weigh 5g of the ginseng powder passed through a 60-mesh sieve, add 250mL of distilled water with a pH value of 3.5, keep it at 30°C for 15min, then add 0.05g of galactosidase to the soaked sample, and incubate at 30°C Enzyme hydrolysis at low temperature for 0.5h, put the mixture in an ultrasonic instrument, ultrasonically extract for 10min in a water bath at 30°C and an ultrasonic power of 180W, then place the mixture at 80°C for 5min to inactivate the enzyme preparation, and then cool down to room temperature, and centrifuged at 3000rpm for 10min, transferred the supernatant, concentrated the obtained extract to 1 / 5 volume with a rotary evaporator, then added absolute ethanol 5 times the volume of the concentrated solution, and stood at 4°C for 2h to precipitate polysaccharides , centrifuged at 5000rpm for 10min, washed again with absolute eth...

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Abstract

The invention discloses Disporum cantoniense polysaccharide extracted by an ultrasound-assisted enzymatic method and an extraction method and application thereof. The extraction method comprises taking 1 part by mass of Disporum cantoniense powder which is sieved by a 60-mesh sieve, soaking the Disporum cantoniense powder in 15-50 parts by mass of distilled water, adjusting the pH value to 3.5-7.0, standing the solution at the temperature of 30-70 DEG C for 15-30 minutes, then adding an enzyme which accounts for 0.1-10% of the mass of the dry powder of the Disporum cantoniense into the solution, carrying out enzymolysis at 30-70 DEG C for 0.5-3 hours to obtain a mixture, carrying out ultrasonic extraction at the temperature of 30-70 DEG C for 10-60 minutes, carrying out enzyme deactivationtreatment at the temperature of 80-90 DEG C for 5-20 minutes, carrying out cooling at the room temperature, centrifuging the mixture at the speed of 3000 to 6000 rpm for 10 to 30 minutes, concentrating the supernatant to 1 / 2-1 / 5 volume by using a rotary evaporator, then adding absolute ethyl alcohol of which the volume is 4-5 times that of the concentrated solution into the supernatant, standingthe solution at 4 DEG C for 2-8 hours, centrifuging the solution at the speed of 5000-6000 rpm for 10-20 minutes, washing the solution by using absolute ethyl alcohol, and freeze-drying the solution to obtain the product. The Disporum cantoniense polysaccharide has toxicity to tumor cells, has good inhibitory activity to tobacco mosaic viruses and cucumber mosaic viruses, and is high in extractionrate, short in extraction time, energy-saving and environment-friendly.

Description

technical field [0001] The present invention relates to the technical field of traditional Chinese medicine, in particular to the polysaccharides of Radix ginseng extracted by ultrasonic-assisted enzymatic method, and also to the method of polysaccharide of Radix ginseng extracted by ultrasonic-assisted enzymatic method, and the polysaccharide of Radix ginseng extracted by ultrasonic-assisted enzymatic method. Applications of polysaccharides in the field of tumor cytotoxicity and antiviral. Background technique [0002] Polysaccharides are natural high molecular polymers formed by connecting more than 10 monosaccharides through glycosidic bonds, and widely exist in plants, animals and microorganisms. It is not only a component of cells, but also an important substance to regulate various life activities. Studies have found that plant polysaccharides have a variety of physiological functions, such as anti-oxidation, anti-fatigue, anti-tumor, anti-coagulation, lowering blood ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A01N65/42A01N43/16A01P1/00A61K31/715A61K36/896A61P35/00
CPCA01N43/16A01N65/00A01N65/42A61K31/715A61K36/896A61P35/00C08B37/00
Inventor 甘秀海宋宝安胡德禹吴剑张建陈吉祥
Owner GUIZHOU UNIV
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