A kind of biphenol compound contained in tobacco rhizome and its preparation method and application

A compound, biphenol technology, applied in the field of tobacco chemistry, can solve the problems that have not been reported, and achieve the effect of good activity, good anti-tobacco mosaic virus activity, and simple structure

Active Publication Date: 2016-04-20
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention has isolated a biphenol compound from tobacco rhizomes, which has not been reported so far

Method used

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  • A kind of biphenol compound contained in tobacco rhizome and its preparation method and application
  • A kind of biphenol compound contained in tobacco rhizome and its preparation method and application
  • A kind of biphenol compound contained in tobacco rhizome and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] ---The preparation method of the compound includes extract extraction, silica gel column chromatography, and high pressure liquid chromatography separation steps, specifically including the following steps:

[0028] 1. Extraction of extract: take tobacco sample, crush it to 30 mesh, extract it with 95% methanol ultrasonically for 4 times, each time for 45 minutes, combine the extracts, filter, and concentrate under reduced pressure to form extract;

[0029] 2. Silica gel column chromatography: the extract is dry-packed with 180 mesh silica gel column with a weight ratio of 2.5 times for silica gel column chromatography; the volume ratio is 1:0, 20:1, 9:1, 8:2, 7:3, 6:4, 1:1, and 1:2 chloroform-acetone solutions were used for gradient elution, the same fractions were combined, and the eluents of each fraction were collected and concentrated;

[0030] 3. High-pressure liquid chromatography separation: the 7:3 part of the eluent in step 2 is further separated and purified ...

Embodiment 2

[0036] The tobacco samples were from Yuxi, Yunnan, and the variety was Yunyan 87. Sampling 2.0 kg of tobacco rhizomes was crushed to 30 mesh, extracted 3 times with 95% methanol by ultrasonic, each time for 30 min, the extracts were combined, filtered, concentrated under reduced pressure to obtain extract, 72.6 g of extract was obtained. After dissolving the extract with 2.5 times the amount of pure methanol by weight, mix the sample with 120g of 100-mesh silica gel, pack 1.5kg of 160-mesh silica gel into a column for silica gel column chromatography, and use a volume ratio of 1:0, 20:1, 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring and merging the same parts to obtain 8 parts, wherein the volume ratio is 7: The chloroform-acetone elution part of 3 was separated by Agilent 1100 semi-preparative high-performance liquid chromatography, with 45% methanol as mobile phase, ZorbaxSB-C18 (21.2×250mm, 5 μm) preparative column as stationary phase, flo...

Embodiment 3

[0038]Tobacco samples are from Dali, Yunnan, and the variety is Yunyan 87. 3.2 kg of tobacco rhizomes are sampled and crushed to 40 meshes, extracted 5 times with 90% methanol by ultrasonic, each extraction takes 45 minutes, the extracts are combined, filtered, and concentrated under reduced pressure to obtain a Ointment, get extractum 126g. The extract is dissolved in pure methanol with a weight ratio of 2.0 times, and then mixed with 300 g of 80-mesh silica gel, and 1.5 kg of 200-mesh silica gel is packed into a column for silica gel column chromatography. The volume ratio is 1:0, 20:1, 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring and merging the same parts to obtain 8 parts, wherein the volume ratio is 7: The chloroform-acetone elution part of 3 was separated by Agilent 1100 semi-preparative high-performance liquid chromatography, with 45% methanol as mobile phase, ZorbaxSB-C18 (21.2×250mm, 5 μm) preparative column as stationary phase, fl...

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Abstract

The invention discloses a diphenol compound (I) in tobacco rhizome and a preparation method and application thereof. Tobacco whole plant is crushed, and then ultrasonically extracted with methanol, the extracted liquid is merged, filtered, and concentrated under vacuum into extract; the extract is primarily separated by silica gel column chromatography; a chloroform-acetone solution with the volume ratio of 1:0 to 1:2 is used for gradient elution; eluent 7:3 part is further separated and purified by high pressure liquid chromatography to obtain the required diphenol compound. The experiment proves that the biphenyl compound has good inhibition effect on tobacco mosaic virus, and can be used as a lead compound against the tobacco mosaic virus.

Description

technical field [0001] The invention belongs to the technical field of tobacco chemistry, in particular to a biphenol compound extracted from tobacco rhizomes for the first time. At the same time, the invention also relates to the preparation method and application of the biphenol compound. Background technique [0002] Tobacco is one of the most abundant chemical substances among the various plants known to mankind. After decades of research, more than 3,000 individual chemical substances have been identified from tobacco, and there are still many components that have not yet been identified. identified. In addition to tobacco being mainly used for smoking cigarettes, a variety of valuable chemical components can also be extracted from it, and leading compounds with development and utilization value can be found from it. In particular, the roots and stems of tobacco are generally treated as waste from the tobacco leaf production process. Therefore, it is of great signifi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C47/575C07C45/78A01N35/04A01P1/00
CPCA01N35/04C07C45/78C07C47/575
Inventor 张涛尚善斋缪明明赵伟陈章玉杨光宇刘春波王岚张凤梅申钦鹏刘志华
Owner CHINA TOBACCO YUNNAN IND
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