Microsensor controllable array preparation method using tobacco mosaic virus as template

A technology of tobacco mosaic virus and micro-sensor, which is applied in the directions of instruments, scientific instruments, measuring devices, etc., can solve the problems of difficult to realize the preparation of complex microstructures and controllable arrays, complex preparation processes, expensive equipment, etc. Sensitivity, increased contact area, low cost effect

Inactive Publication Date: 2018-12-07
NORTHWESTERN POLYTECHNICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This traditional method involves complex preparation processes and expensive equipment, and it is difficult to realize the preparation and controllable array of complex microstructures

Method used

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  • Microsensor controllable array preparation method using tobacco mosaic virus as template
  • Microsensor controllable array preparation method using tobacco mosaic virus as template
  • Microsensor controllable array preparation method using tobacco mosaic virus as template

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Preparation method of piezoelectric sensor array based on tobacco mosaic virus

[0043] Step 1: Use phage display technology to obtain polypeptides and corresponding gene sequences that can specifically bind to barium titanate (BTO) piezoelectric nanoparticles

[0044] A: Take an appropriate amount of barium titanate (BTO) nanoparticle dispersion, place it in the buffer solution containing the M13 phage library, shake and mix at room temperature for 2 hours, so that the M13 phage and barium titanate nanoparticles are fully mixed;

[0045] B: centrifuge the above mixed solution for 40min at 3500rpm, remove the supernatant;

[0046] C: Rinse the precipitate 3 times with a buffer to remove the phages that are not firmly adsorbed;

[0047] D: Elute the phage that adsorbs specific functional nanoparticles, and place it in Escherichia coli culture solution for enrichment and cultivation for 30 minutes;

[0048] E: Perform high-throughput sequencing on the enriche...

Embodiment 2

[0072] Example 2: Preparation method of ammonia sensor array based on tobacco mosaic virus

[0073] Step 1: Use phage display technology to obtain a compound that can specifically bind to titanium dioxide (TiO 2 ) polypeptides of piezoelectric nanoparticles and their corresponding gene sequences

[0074] A: Take an appropriate amount of titanium dioxide (TiO 2 ) nanoparticle dispersion, which is placed in the buffer containing the M13 phage library, and shaken and mixed at room temperature for 2 hours to make the M13 phage and titanium dioxide (TiO 2 ) nanoparticles are fully mixed;

[0075] B: centrifuge the above mixed solution for 40min at 3500rpm, remove the supernatant;

[0076] C: Rinse the precipitate 3 times with a buffer to remove the phages that are not firmly adsorbed;

[0077] D: Elute the phage that adsorbs specific functional nanoparticles, and place it in Escherichia coli culture solution for enrichment and cultivation for 30 minutes;

[0078] E: Perform hi...

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Abstract

The invention discloses a microsensor-controllable array preparation method using tobacco mosaic virus as a template. According to the method, a phage display technology is utilized to obtain a polypeptide molecule that binds to a specific functional nanoparticle; and a gene sequence corresponding to the polypeptide molecule is obtained by gene sequencing. Subsequently, the tobacco mosaic virus isgenetically modified by a molecular biological means, and the polypeptide molecule that binds to the specific functional nanoparticle is adsorbed on coat protein to bind to the specific functional nanoparticle to obtain sensitive units; and at the same time, cysteine (Cys) is introduced into substrate surface protein, by using a binding mechanism of the cysteine and gold (Au) nanoparticles, arraydistribution of the sensitive units of a sensor can be achieved. The method using the tobacco mosaic virus as the template to realize sensor controllable array can greatly reduce an array unit size,improves the resolution and sensitivity of the sensor, and can be applied to array design and preparation of pressure sensors and gas sensors.

Description

technical field [0001] The invention belongs to the technical field of microsensor manufacturing, and more specifically relates to a microsensor controllable array preparation method using tobacco mosaic virus as a template. Background technique [0002] With the continuous development and progress of industrialization and information technology, higher requirements are put forward for sensor technology. At present, sensor technology is developing in the direction of miniaturization, integration, intelligence, high precision and high sensitivity. The traditional microsensor manufacturing process is mainly based on silicon-based processing technology, including photolithography, wet / dry etching, thinning polishing, bonding, etc., which belongs to the "top-down" processing technology. This traditional method involves complex preparation processes and expensive equipment, and it is difficult to realize the preparation and controllable array of complex microstructures. [0003...

Claims

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Application Information

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IPC IPC(8): G01N33/569
CPCG01N33/56983
Inventor 马炳和张浩王璇甫苑伟政姜澄宇邓进军罗剑
Owner NORTHWESTERN POLYTECHNICAL UNIV
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