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A controllable array preparation method of microsensors using tobacco mosaic virus as a template

A tobacco mosaic virus and micro-sensor technology, applied in instruments, scientific instruments, measuring devices, etc., can solve the problems of expensive equipment, complex preparation process, difficult to achieve complex microstructure preparation and controllable arraying

Inactive Publication Date: 2021-05-07
NORTHWESTERN POLYTECHNICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This traditional method involves complex preparation processes and expensive equipment, and it is difficult to realize the preparation and controllable array of complex microstructures

Method used

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  • A controllable array preparation method of microsensors using tobacco mosaic virus as a template
  • A controllable array preparation method of microsensors using tobacco mosaic virus as a template
  • A controllable array preparation method of microsensors using tobacco mosaic virus as a template

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Preparation method of piezoelectric sensor array based on tobacco mosaic virus

[0043] Step 1: Use phage display technology to obtain polypeptides and corresponding gene sequences that can specifically bind to barium titanate (BTO) piezoelectric nanoparticles

[0044] A: Take an appropriate amount of barium titanate (BTO) nanoparticle dispersion, place it in the buffer solution containing the M13 phage library, shake and mix at room temperature for 2 hours, so that the M13 phage and barium titanate nanoparticles are fully mixed;

[0045] B: centrifuge the above mixed solution for 40min at 3500rpm, remove the supernatant;

[0046] C: Rinse the precipitate 3 times with a buffer to remove the phages that are not firmly adsorbed;

[0047] D: Elute the phage that adsorbs specific functional nanoparticles, and place it in Escherichia coli culture solution for enrichment and cultivation for 30 minutes;

[0048] E: Perform high-throughput sequencing on the enriche...

Embodiment 2

[0072] Example 2: Preparation method of ammonia sensor array based on tobacco mosaic virus

[0073] Step 1: Use phage display technology to obtain a compound that can specifically bind to titanium dioxide (TiO 2 ) polypeptides of piezoelectric nanoparticles and their corresponding gene sequences

[0074] A: Take an appropriate amount of titanium dioxide (TiO 2 ) nanoparticle dispersion, which is placed in the buffer containing the M13 phage library, and shaken and mixed at room temperature for 2 hours to make the M13 phage and titanium dioxide (TiO 2 ) nanoparticles are fully mixed;

[0075] B: centrifuge the above mixed solution for 40min at 3500rpm, remove the supernatant;

[0076] C: Rinse the precipitate 3 times with a buffer to remove the phages that are not firmly adsorbed;

[0077] D: Elute the phage that adsorbs specific functional nanoparticles, and place it in Escherichia coli culture solution for enrichment and cultivation for 30 minutes;

[0078] E: Perform hi...

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Abstract

The invention discloses a microsensor controllable array preparation method using tobacco mosaic virus as a template. The method utilizes phage display technology to obtain a polypeptide molecule combined with specific functional nanoparticles; and obtains the gene sequence corresponding to the polypeptide molecule through gene sequencing. Subsequently, the tobacco mosaic virus was genetically modified by means of molecular biology, and the above-mentioned polypeptide molecules combined with specific functional nanoparticles were adsorbed on its coat protein, which was used to bind functional nanoparticles to obtain sensitive units; Cysteine ​​(Cys) is introduced, and the combination mechanism of cysteine ​​and gold (Au) nanoparticles is used to realize the array distribution of sensor sensitive units. This method of using tobacco mosaic virus as a template to realize the controllable arraying of sensors can greatly reduce the size of the array unit, improve the resolution and sensitivity of the sensor, and is applicable to the array design and preparation of pressure sensors and gas sensors.

Description

technical field [0001] The invention belongs to the technical field of microsensor manufacturing, and more specifically relates to a microsensor controllable array preparation method using tobacco mosaic virus as a template. Background technique [0002] With the continuous development and progress of industrialization and information technology, higher requirements are put forward for sensor technology. At present, sensor technology is developing in the direction of miniaturization, integration, intelligence, high precision and high sensitivity. The traditional microsensor manufacturing process is mainly based on silicon-based processing technology, including photolithography, wet / dry etching, thinning polishing, bonding, etc., which belongs to the "top-down" processing technology. This traditional method involves complex preparation processes and expensive equipment, and it is difficult to realize the preparation and controllable array of complex microstructures. [0003...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569
CPCG01N33/56983
Inventor 马炳和罗剑李博源苑伟政王冠邓进军毕珍
Owner NORTHWESTERN POLYTECHNICAL UNIV
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