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Method for preparing tobacco mosaic virus capsid protein capsule

A technology of tobacco mosaic virus and capsid protein, which is applied in the field of biomedicine, can solve the problem of no tobacco mosaic virus capsid protein, etc., and achieve the effect of strong capsule structure, simple preparation method and broad application prospects

Active Publication Date: 2017-06-13
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there is no research report on the preparation of protein capsules prepared by the assembly of tobacco mosaic virus capsid proteins at the emulsion interface

Method used

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  • Method for preparing tobacco mosaic virus capsid protein capsule
  • Method for preparing tobacco mosaic virus capsid protein capsule
  • Method for preparing tobacco mosaic virus capsid protein capsule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 A kind of preparation method of tobacco mosaic virus capsid protein capsule

[0038] A kind of preparation method of tobacco mosaic virus capsid protein capsule comprises the following steps (its flow chart is as follows figure 1 shown):

[0039] (1) Preparation of tobacco mosaic virus capsid protein solution

[0040] Take 0.6ml of tobacco mosaic virus solution with a concentration of 10mg / ml, add 1.2ml of acetic acid to it, invert the centrifuge tube 5 times to mix well, and then let it stand in a refrigerator at 4°C for 30min. Centrifuge at 9500 rpm for 10 min at 4°C to remove the RNA pellet. The supernatant was separated through a desalting column to remove the acetic acid fraction. The resulting solution was dialyzed against deionized water for 18 h, after which it was centrifuged at 9000 rpm for 10 min at 4°C. The obtained precipitate was redissolved by adding 100 mM phosphate buffer solution with pH=8.0 to obtain a tobacco mosaic virus capsid prot...

Embodiment 2

[0045] Embodiment 2 A kind of preparation method of tobacco mosaic virus capsid protein capsule

[0046] A preparation method of tobacco mosaic virus capsid protein capsule, comprising the following steps:

[0047] (1) Preparation of tobacco mosaic virus capsid protein solution

[0048]Take 0.6ml of tobacco mosaic virus solution at a concentration of 20mg / ml, add 1.2ml of acetic acid to it, invert the centrifuge tube 5 times to mix well, and then let it stand in a refrigerator at 4°C for 40min. Centrifuge at 10,000 rpm for 8 min at 4°C to remove the RNA pellet. The supernatant was separated through a desalting column to remove the acetic acid fraction. The resulting solution was dialyzed against deionized water for 12 h and then centrifuged at 8000 rpm for 12 min at 4°C. The obtained precipitate was redissolved by adding 200 mM phosphate buffer solution with pH=8.0 to obtain a tobacco mosaic virus capsid protein solution.

[0049] (2) Preparation of Tobacco Mosaic Virus Ca...

Embodiment 3

[0053] Embodiment 3 A kind of preparation method of tobacco mosaic virus capsid protein capsule

[0054] A preparation method of tobacco mosaic virus capsid protein capsule, comprising the following steps:

[0055] (1) Preparation of tobacco mosaic virus capsid protein solution

[0056] Take 0.6ml of tobacco mosaic virus solution with a concentration of 5mg / ml, add 1.2ml of acetic acid to it, invert the centrifuge tube 5 times to mix well, and then let it stand in a refrigerator at 4°C for 30min. Centrifuge at 8000 rpm for 10 min at 4°C to remove the RNA pellet. The supernatant was separated through a desalting column to remove the acetic acid fraction. The resulting solution was dialyzed against deionized water for 24 h and then centrifuged at 9000 rpm for 10 min at 4°C. The obtained precipitate was redissolved by adding 20 mM phosphate buffer solution with pH=8.0 to obtain a tobacco mosaic virus capsid protein solution.

[0057] (2) Preparation of Tobacco Mosaic Virus Ca...

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Abstract

The invention discloses a method for preparing a tobacco mosaic virus capsid protein capsule. The method comprises the following steps: preparing a tobacco mosaic virus capsid protein solution, mixing the tobacco mosaic virus capsid protein solution with an oil phase to form an emulsion, adjusting the pH value of the system to control the tobacco mosaic virus capsid protein to form a rod-shaped combined structure, and cross-linking the rod-shaped tobacco mosaic virus capsid protein at an oil-water boundary by using glutaraldehyde as a cross-linking agent, thereby obtaining the tobacco mosaic virus capsid protein capsule. The method for preparing the tobacco mosaic virus capsid protein, which is disclosed by the invention, is simple in step, short in preparation cycle, high in surface wrapping rate of prepared protein capsules, and solid and uniform in capsule structure.

Description

technical field [0001] The invention relates to the field of biomedicine. More specifically, it relates to a preparation method of tobacco mosaic virus capsid protein capsules. Background technique [0002] In order to meet the challenges of controlled release and targeted delivery of active substances in the field of biomedicine, it is necessary to provide high-efficiency and durable drug loading methods. Traditional drug loading methods are increasingly unsuitable for the application of existing clinical drugs due to low solubility, high toxicity, and easy interaction with other substances. [0003] A protein capsule is a polymer shell with a micron or nanometer size on the outside and an active substance inside. It uses protein as a precursor to obtain a delivery material with low immunogenicity, and can specifically deliver drugs to Diseased tissue without affecting healthy tissue is one of the most promising delivery tools for controlled release. [0004] Microemulsi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/48A61K47/42C08J3/24C08L89/00
Inventor 牛忠伟王兆程
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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