Nano hydroxy apalite/ fibroin-chitosan compound scaffold and its preparation method

A technology of nano-hydroxyapatite and silk fibroin, applied in medical science, prostheses, coatings, etc., can solve problems such as poor connectivity of stomata, unfavorable adhesion, differentiation, proliferation and growth of bone cells, and low porosity , to achieve good stomatal connectivity, good bone regeneration function, and high porosity

Inactive Publication Date: 2006-10-11
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] In order to solve the existing methods of preparing scaffolds, the introduction of toxic organic solvents damages the biological activity of the material, the internal structure of the material has small pores, low porosity, poor connectivity of pores, and is not conducive to the adhesion, differentiation, proliferation and growth of bone cells. The invention provides a nano-hydroxyapatite / silk fibroin-chitosan composite scaffold and a preparation method thereof

Method used

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  • Nano hydroxy apalite/ fibroin-chitosan compound scaffold and its preparation method

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specific Embodiment approach 1

[0021] Embodiment 1: The material of the nano-hydroxyapatite / silk fibroin-chitosan composite scaffold in this embodiment is composed of inorganic materials and organic materials. The inorganic material is nano-hydroxyapatite, and the organic material is For silk fibroin and chitosan, the weight ratio of inorganic material to organic material is 50-70:30-50, and the weight ratio of silk fibroin to chitosan is 1:1-2.

[0022] In this embodiment, the nano-hydroxyapatite is weakly crystalline, with a diameter of 4-6 nm and a length of 20-40 nm. The porosity of the composite support is 75%-90%, and the pore size is 150-500 μm.

specific Embodiment approach 2

[0023] Embodiment 2: This embodiment is different from Embodiment 1 in that the weight ratio of the inorganic material to the organic material is 50:50.

specific Embodiment approach 3

[0024] Embodiment 3: This embodiment is different from Embodiments 1 and 2 in that the weight ratio of the inorganic material to the organic material is 70:30.

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PUM

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Abstract

The invention relates to a method for preparing combined supporting frame of nanometer hydroxyapatite and silk fibroin-chitosan, belonging to field of tissue organizing technology. It is to solve problems of damage of biological activity of material by introduced organic dissolvent, small porous size and low porous rate inside material, bad porous connectivity and unfavorable for reproduction of bone cell in current preparation method. The material in this invention comprises inorganic and organic material, inorganic material is nanometer hydroxyapatite, and organic material is silk fibroin and chitosan. The ratio by weight between inorganic and organic material is 50-70:30-50, the ratio between silk fibroin and chitosan by weight is 1:1-2.The said supporting frame is prepared by freezing and drying method. The cell frame porous rate is 75%-90%, the pore size is 150-500 um, and compression strength is 0.85-1.85 Mpa.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and relates to a nano-hydroxyapatite / silk fibroin-chitosan composite scaffold and a method for preparing the composite scaffold by freeze-drying. Background technique [0002] When human tissues and organs are damaged, the ideal treatment method is to replace them with identical organs. Based on this, tissue engineering techniques have been developed in the past decade. The process of using tissue engineering technology to repair tissue and organ defects is to adsorb tissue cells on the frame material with good biocompatibility and gradually degraded and absorbed by the human body, and at the same time, cells (under the control of growth factors) gradually reproduce human tissues . The scaffold material provides a living three-dimensional space for the cells, which is beneficial for the cells to obtain sufficient nutrients, so that the cells can grow on the pre-designed three-dimensi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/32A61L27/34
Inventor 王静温广武孟庆昌李慕勤孟祥才
Owner HARBIN INST OF TECH
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