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Determining method of artemislnin content in artemisia apiacea

A technique for the determination of artemisinin in artemisinin, which is applied in the field of determination of artemisinin content in Artemisia annua by ultraviolet spectrophotometry, can solve the problems of being unsuitable for on-site rapid quality evaluation, unsuitable for rapid quality evaluation, and high-level equipment. Achieve the effect of shortening the extraction time, rapid detection and convenient operation

Inactive Publication Date: 2007-01-31
CHONGQING MEDICAL UNIVERSITY
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Problems solved by technology

Such as high-performance liquid chromatography, thin-layer chromatography, supercritical fluid chromatography, and photometric enzyme-linked immunoassay. These methods are mainly used for the determination of artemisinin with high content and high purity, but require high equipment, The operation is complicated and time-consuming, which is not suitable for rapid on-site quality evaluation; in addition, there is ultraviolet spectrophotometry, which is mainly for the detection of artemisinin with high purity. Although the operation is simple, it takes a long time and is not suitable for direct on-site rapid quality evaluation. Evaluation

Method used

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  • Determining method of artemislnin content in artemisia apiacea

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Experimental program
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Effect test

preparation example Construction

[0028] 1.2.3 Preparation of Artemisia annua sample solution.

[0029] a. Take Artemisia annua and put it into a pulverizer to pulverize, pass through 80 and 60 mesh sieves respectively, and obtain 60-80 mesh Artemisia annua powder;

[0030] b. Weigh 0.5±0.01g of Artemisia annua samples (60-80 meshes), add 15ml of petroleum ether (30-60°C) to 150ml with a stopper cone, and sonicate for 15min;

[0031] c. After ultrasonication, pour the petroleum ether solution into the graduated cylinder, and record the volume V of petroleum ether;

[0032] d. Precisely measure 0.3ml of the petroleum ether solution that has been ultrasonically treated in a 10ml graduated test tube, put it into boiling water that is no longer heated, and evaporate to dryness.

[0033] 1.2.4 Derivation of Artemisinin

[0034] a. Add 3ml of 95% ethanol to the evaporated test tube in 1.2.3 and d to dissolve the residue, dilute to 10ml with 0.2% NaOH solution, mix well, and bathe in water at 50±1℃ for 30min;

[0...

Embodiment 2

[0062] Example 2: In this example, except that the time for the incomplete extraction of the same Artemisia annua medicinal material is 5, 12, 15, 20, 25, and 30 minutes, other extractions and ultraviolet spectroscopic The detection method of the photometric analysis method is the same, and the measured artemisinin content data of the same kind of Artemisia annua medicinal material under different extraction time conditions are shown in Table 2, and the change curve of incomplete extraction extraction time and artemisinin content is shown figure 2 . from figure 2 It can be seen that the measured artemisinin content data is basically stable within the extraction time range of 12 to 17 minutes.

[0063] Extraction time min

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Abstract

The invention relates to a test method for arteannuin content in southernwood that needs not fully distill the arteannuin in southernwood. It adopts ultraviolet spectrum luminosity analysis method to test the arteannuin content data M (mg / g), and substituting to the linear model built up from the corresponding relationship of arteannuin content data tested by liquid chromatography after fully distilling the arteannuin, and calculating the actual content X (mg / g) in southernwood. The invention simplifies the operation process, shortens distilling time and could finish test in 1.5h. The actual content data of arteannuin is reliable. The invention could also realize rapid test content of arteannuin in southernwood purchasing locale.

Description

technical field [0001] The invention relates to a method for determining the content of artemisinin in Artemisia annua, in particular to a method for determining the content of artemisinin in Artemisia annua by using ultraviolet spectrophotometry. Background technique [0002] Artemisinin and artemisinin-based antimalarial drugs such as artemether, dihydroartemisinin, and artesunate synthesized from artemisinin have been recommended by the World Health Organization as the best possible substitute for quinine in the treatment of malaria. Safe and effective medicines. Since the amount of artemisinin contained in Artemisia annua medicinal materials produced in different regions varies greatly (2‰~12‰), which has obvious ecological regional characteristics, it is necessary to carry out quality control on medicinal materials, especially when purchasing medicinal materials Therefore, both the supplier and the buyer need a method to quickly evaluate the quality of Artemisia annua,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/27G01N30/86G01N1/34G01N1/28G06F19/00
Inventor 尚京川杨俊卿母昭德蒋青松周歧新
Owner CHONGQING MEDICAL UNIVERSITY
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