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Liquid phase chromatographic method for simultaneously measuring compound sulfamethoxazole and metabolite thereof

A compound sulfonamide and liquid chromatography technology, which is applied in the field of determination of sulfonamide antibacterial drugs, achieves good extraction effect, high accuracy and precision, and reduces detection cost

Inactive Publication Date: 2007-02-07
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

Most of these related reports only involve the detection of sulfa drugs themselves, and do not involve the simultaneous detection of the synergist trimethoprim and acetylated metabolites, and the sample pretreatment method adopted is also different from the present invention

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  • Liquid phase chromatographic method for simultaneously measuring compound sulfamethoxazole and metabolite thereof
  • Liquid phase chromatographic method for simultaneously measuring compound sulfamethoxazole and metabolite thereof

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Embodiment Construction

[0028] Describe technical content of the present invention in detail below in conjunction with accompanying drawing by embodiment:

[0029] Its technical methods include: sample preparation, sample pretreatment, standard solution preparation, mobile phase preparation and chromatographic conditions:

[0030] A. Sample preparation: Collect fish muscle tissue samples, cut them into pieces with scissors, homogenize them with a high-speed homogenizer, put them into plastic centrifuge tubes, seal them and store them in a freezer.

[0031] B. Sample pretreatment: Take 15 15ml plastic centrifuge tubes, add 1g of thawed blank muscle tissue respectively, put them into plastic centrifuge tubes, add standard solutions of different concentrations (0.5, 2.0, 10.0, 50.0, 200.0μg / mL ) each 0.1mL, each concentration set 3 parallel, so that the concentrations of the three drugs in 1g of muscle tissue reached 0.05, 0.20, 1.00, 5.00, 20.00μg / g, mixed thoroughly, let stand for 4 hours, added 0.3mo...

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Abstract

The liquid phase chromatogram method for detect both compound sulfathiazole and its metabolite comprises: using the mixed liquid with HCl and 3:1 CH2Cl2 / acetone as the extractant to extract target with less impurity; after adding the extractant, keeping for some time in 4Deg icebox to modify protein fully and reduce loss and improve recovery yield; drying the solvent with N2, filtering, and detecting. This invention is simple, fast and very sensitive, and has wide application.

Description

technical field [0001] The present invention relates to the determination method of sulfonamide antibiotics; Specifically, adopt high-performance liquid chromatography to measure sulfamethoxazole (SMZ), acetylated metabolite (acetylated sulfamethoxazole, NSMZ) and methoxymethoxazole simultaneously in fish meat Benzidine (TMP) method. Background technique [0002] Sulfamethoxazole (also known as sulfamethoxazole) is a widely used drug in the prevention and control of aquaculture diseases. It is usually used in combination with the sulfa synergist trimethoprim; In addition to azoles, acetylated sulfamethoxazole is its main metabolite, which remains in the body for a long time. Therefore, it is very necessary to detect the content of acetylated metabolites and synergists when studying the residues of sulfonamides in aquatic products. At present, the detection of sulfa drugs such as sulfamethoxazole mostly uses high-performance liquid chromatography, and the relevant reports a...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N1/28G01N30/60
Inventor 王群李健刘淇何玉英
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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