Stabilized enzyme formulations

A technology of enzyme preparation and stabilizer, which is applied in the field of preparation of such enzyme-containing granules and liquid preparations, and can solve the problems of insufficient inherent resistance

Inactive Publication Date: 2007-02-14
BASF SE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most industrial enzymes currently available for feed and / or food applications have insufficient intrinsic resistance to heat inactivation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment V1

[0245] Embodiment V1 (reference)

[0246] Preparation of cornstarch-based enzyme granules by kneading, extrusion, spheronization and drying

[0247] In the first step, cornstarch was filled into a laboratory mixer (Loedige Typ M5 RMK) and stirred slowly. Ultrafiltrate containing phytase, ZnSO 4 x6 2 A mixture of O, PVA (polyvinyl alcohol) and additional water was poured into the beaker and dissolved / dispersed. Pour this mixture over the cornstarch in the Loedige mixer.

[0248] Then, by mixing and kneading 66% (w / w) cornstarch, 20% (w / w) phytase-containing ultrafiltrate, 1% ( w / w) PVA, and 0.3% (w / w) ZnSO 4 x6 2 O and 12.7% (w / w) additional water, an enzyme-containing dough was obtained.

[0249] In the second step, the enzyme-containing dough was extruded using a Fuji Paudal laboratory extruder model DG-L1 to obtain a wet extrudate, which was then rounded in a laboratory spheroniser, Round particles with an average diameter between 500-800 μm are obtained.

[0250] ...

Embodiment 1

[0251] Example 1: Soy protein as a stabilizer

[0252] Enzyme (phytase)-containing granules were obtained according to Example V1. Soy protein (Supro 670; name: IP Non GM; Lot No. IP 094 / 2003, distributed by Protein Technologies International, CAS No. 9010-10-0) was used instead of PVA.

[0253] Example 1

Embodiment 2

[0254] Example 2: Soy protein as a stabilizer

[0255] Enzyme (phytase)-containing granules were obtained according to Example V1. Soy protein Supro 1751 (Lot No. M 330000575, distributed by Protein Technologies International, CAS No. 9010-10-0) was used instead of PVA.

[0256] Example 2

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Abstract

The invention relates to a stabilized solid or liquid enzyme formulation comprising at least one enzyme and at least one stabilizing agent selected from the group consisting of gummi arabicum, at least one plant protein and mixtures thereof. The invention also relates to processes for producing these formulations, especially to solid formulations (e.g. granules) and their use in animal and / or human nutrition.

Description

technical field [0001] The present invention relates to solid or liquid enzyme preparations with increased stability, preferably thermostability, obtained by adding stabilizers. [0002] The present invention relates to the formulation of enzymes, especially feed-enzymes, into carbohydrate (eg starch-) containing granules and / or liquid formulations and to methods of preparing such enzyme-containing granules and liquid formulations. These (edible) granules and liquid formulations can then be used in animal feed and / or human nutrition. The enzyme-containing granules and liquid formulations exhibit improved storage and processing stability. Background of the invention [0003] The use of various enzymes in human nutrition or animal (eg livestock) feed has become a nearly universal practice. These enzymes are usually produced by growing microorganisms in large fermenters operated by industrial enzyme producers. At the end of the fermentation, usually the resulting "broth" is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/165C12N9/16C12N9/98A23L1/00A23L29/00C12N9/96
CPCA23K1/004A23L1/0029A23K1/001A23L1/034A23K1/003A23K1/002A23K1/1653C12N9/96A23K40/00A23K40/10A23K40/20A23K40/30A23K20/189A23P10/30A23L29/06A23K40/25A23K20/147A23K20/163A23K10/30
Inventor A·哈比希J·布劳恩
Owner BASF SE
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