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Compositions and methods for growth of embryonic stem cells

A stem cell and composition technology, applied in the field of molecular and cell biology, can solve the problem of insufficient prevention of hESC differentiation

Inactive Publication Date: 2007-04-18
RGT UNIV OF CALIFORNIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, results are increasingly showing that the substances required for hESC growth are quite different from those required for mESC growth
For example, it was recently found that, unlike mESCs, activation of STAT3 does not sufficiently prevent hESC differentiation when cultured on mEFs or using mEFs-conditioned media4

Method used

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  • Compositions and methods for growth of embryonic stem cells
  • Compositions and methods for growth of embryonic stem cells
  • Compositions and methods for growth of embryonic stem cells

Examples

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Embodiment

[0140] [137] The present invention will be further explained by the following examples, the purpose of which is to illustrate the present invention clearly, but not to limit the present invention in any way.

[0141] [138] Example 1: Maintenance of pluripotency of stem cells in the absence of feeder cells or conditioned medium.

[0142] [139] The present invention relates to compositions and methods for maintaining the undifferentiated state and pluripotency of embryonic stem cells, particularly undifferentiated human embryonic stem cell lines, using enriched TGFβ family members, FGF family members, and / or NIC. The example is that in the presence of activin A, a member of the TGFβ family, the pluripotency of stem cells is maintained, and the addition of keratinocyte growth factor (KGF), a member of the FGF family, prolongs the time for cell growth or aging. The examples also show that the addition of NIC to the culture medium can prolong the time for cells to grow or enter sen...

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Abstract

The present invention relates to methods, compositions, and kits for maintenance of the undifferentiated state and / or pluripotency in stem cells, including embryonic stem (ES) cells. It also relates to stem cells that have been maintained in an undifferentiated state. The present invention provides these aspects using culture medium enriched with a TGFbeta family member protein, an FGF family member protein, and / or nicotinamide, without using fibroblast feeder layers, conditioned media, or leukemia inhibitory factor.

Description

[0001] Cross reference to related applications [0002] [001] This application requires the priority of provisional patent application NO.US60 / 552,318, the date of application being March 10, 2004, and all the disclosed content should be considered as a whole. technical field [0003] [002] The present invention relates to the fields of molecular and cell biology, especially the cultivation of embryonic stem cells in an undifferentiated state, and the use of these cells for the research of cell production process and the development of medical products. Background technique [0004] [003] Due to the pluripotency of embryonic stem cells, it has great potential in the treatment of many human diseases. Embryonic stem cells are an important resource that, due to their ability to differentiate into many cell types, impact research and development of treatments for diseases and conditions of various cell types, tissues and organs. Therefore, the focus of current research is on re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00C12N5/0735
CPCC12N2501/117C12N2500/38C12N2501/16C12N2533/52C12N5/0606A61P35/00A61P43/00
Inventor 阿尔伯特·哈耶克吉莉安·贝蒂安娜·洛佩斯
Owner RGT UNIV OF CALIFORNIA
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