Pharmaceutical composition having preventative or treatment effect on inflammatory bowel diseases comprising stemonae radix extract
a technology of stemonae radix and composition, which is applied in the direction of digestive system, medical preparations, analgesics, etc., can solve the problems of limiting the use of these drugs, the development of therapeutic drugs has not yet been accomplished, and the expression level of mmp12 is reduced, and the effect of suppressing the decrease of lactobacilli
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example 1
a Stemonae Radix Extract on Cell Line
example 1-1
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[0057]First, dried powder of a Stemonae Radix hot-water extract purchased from SUN TEN (Taipei, Taiwan) was dissolved in water, and filtered to obtain a liquid extract of Stemonae Radix (extract of root of Stemona japonica Miguel, ERSJM).
[0058]Meanwhile, the RAW 264.7 cell line purchased from the Korean Cell Line Bank (KCLB) was inoculated into a DMEM medium (10% FBS, 1% physiological saline), and cultured in an incubator with 5% CO2 maintained at 37° C.
example 1-2
of Cell Toxicities of Stemonae Radix Extract
[0059]The RAW 264.7 cell line cultured in Example 1 was aliquoted into a 96-well plate at a concentration of 2×105 cells / well, added to a DMEM medium, and cultured in an incubator with 5% CO2 maintained at 37° C. Then, the medium was removed from the cultured cells, replenished with a DMEM medium containing Stemonae Radix extracts at concentrations of 0, 1, 10 or 100 μg / mL without FBS, and recultured in an incubator with 5% CO2 maintained at 37° C. for 24 hours. Upon completion of culturing, 50 μL of MTT sol. (2 mg / mL) was added to each well, allowed to react in an incubator with 5% CO2 maintained at 37° C. for 4 hours, the medium was removed from each well, added with 200 μL of DMSO, and their respective absorbance was measured using an ELISA reader. The thus-obtained absorbance was compared to evaluate the cell toxicities of the Stemonae Radix extracts (FIG. 1).
[0060]FIG. 1 is a graph illustrating the evaluation result of cell toxicities...
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