Unlock instant, AI-driven research and patent intelligence for your innovation.
Assay device and method
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
a technology of assay device and method, which is applied in the field of diagnostics and detection, can solve the problems of centralized laboratories and large hospitals, the limitation of such effort, and the cost of analyzers
Inactive Publication Date: 2002-06-20
NAGAOKA
View PDF0 Cites 8 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Benefits of technology
[0043] The spatial addressability of signal elements on the assay device permits identification of analytes bound to distinct signal elements, including identification of multiple analytes in a single assay.
[0044] The invention thus provides, in one embodiment, nucleic acid hybridization assays, in which the first and second side elements of the cleavable signal elements include oligonucleotides. Simultaneous binding of a nucleic acid present in the assay sample to the first and second side elements of the cleavable signal element prevents loss, through cleavage, of the signal element's signal-responsive end.
[0046] The invention further provides immunoassays. In these embodiments, the specificity-conferring side elements of the cleavable signal elements include antibodies, antibody fragments, or antibody derivatives. Simultaneous binding of an analyte to the antibody of the first side element and the antibody of the second side element prevents the loss, through cleavage, of the signal element's signal-responsive end.
Problems solved by technology
However, these analyzers are expensive and only centralized laboratories and large hospitals can afford them.
Such centralization necessitates sample transport, and often precludes urgent or emergent analysis of time-critical samples.
The limit of such effort is the design of clinical tests suitable for use at the patient bedside or in the patient's home without dedicated detectors.
Otherwise, all these methods are only qualitative.
One of the newest is a thermometer-type assay device (Ertinghausen G., U.S. Pat. No. 5,087,556) that is not yet commercially available.
One disadvantage, however, of each of these formats is that only one, or a very limited number, of assays can conveniently be performed simultaneously.
Each cassette has a relatively complicated structure.
The indentations on the disk cause destructive interference within the reflected beam, which corresponds to a bit having a "zero" value.
While the smaller wavelength is backward compatible with standard pressed CDs, it is incompatible with current versions of the dye-based CD-R.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
example i
6. EXAMPLE I
Increasing the Specificity of a Nucleic Acid Hybridization Assay
[0249] In a direct nucleic acid hybridization assay, the side elements of the cleavable signal element are oligonucleotides designed to hybridize with distinct sites on a chosen, predetermined, nucleic acid to be detected in the sample. For many applications of this methodology, cross-reactivity with sample oligonucleotides having even a single mismatched nucleotide should be minimized. In particular, nucleic acid hybridization assays adapted to use the cleavable reflective signal element of the present invention for detection of point mutations, as, e.g., for detection of point mutations in the BRCA1 and BRCA2 genes that predispose to breast and ovarian cancers, must be able to discriminate as between nucleic acid samples containing a single mismatched nucleotide.
[0250] The longer the oligonucleotide side elements of the cleavable signal element--and thus the longer the sequence that is complementary as bet...
example ii
7. EXAMPLE II
Detection of HIV-1
[0265] HIV-1 proviral DNA from clinical samples is amplified as follows, essentially as described in U.S. Pat. No. 5,599,662, incorporated herein by reference.
[0266] Peripheral blood monocytes are isolated by standard Ficoll-Hypaque density gradient methods. Following isolation of the cells, the DNA is extracted as described in Butcher and Spadoro, Clin. Immunol. Newsletter 12:73-76 (1992), incorporated herein by reference.
[0267] Polymerase chain reaction is performed in a 100 .mu.l reaction volume, of which 50 .mu.l is contributed by the sample. The reaction contains the following reagents at the following initial concentrations:
[0268] 10 mM Tris-HCl (pH 8.4)
[0269] 50 mM KCl
[0270] 200 .mu.M each DATP, dCTP, dGTP, and dUTP
[0271] 25 pmoles of primer 1, of sequence shown below
[0272] 25 pmoles of primer 2, of sequence shown below
[0273] 3.0 mM MgCl.sub.2
[0274] 10% glycerol
[0275] 2.0 units of Taq DNA polymerase (Perkin-Elmer)
[0276] 2.0 units UNG (Perkin-Elm...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Property
Measurement
Unit
diameter
aaaaa
aaaaa
diameter
aaaaa
aaaaa
thickness
aaaaa
aaaaa
Login to View More
Abstract
A cleavable signal element for use in quantitative and qualitative assay devices and methods is described. Binding of the chosen analyte simultaneously to a first and a second analyte-specific side member of the cleavable signal element tethers the signal-responsive moiety to the signal element's substrate-attaching end, despite subsequent cleavage at the cleavage site that lies intermediate the first and second side members. Assay devices comprising the cleavable signal elements are described, as are analytic methods adapted to their use. The analytic devices of the present invention may be adapted to detection using conventional CD-ROM and DVD readers.
Description
[0001] The present application is a continuation-in-part of U.S. provisional application No. 60 / 021,367, filed Jul. 8, 1996 and U.S. provisional application No. 60 / 030,416, filed Nov. 1, 1996, incorporated herein by reference.1. INTRODUCTION[0002] The present invention relates to the field of diagnostics and the detection of small quantities of substances in fluids. More specifically, the invention relates to a cleavable signal element, particularly a cleavable reflective signal element, for use in assay devices. The assay devices employing such signal elements are, in preferred embodiments of the invention, adapted for detection using standard laser-based detection systems, such as CD-ROM readers, DVD readers, and the like. The invention further includes analytical methods for detecting analytes using the assay devices of the present invention. The signalling element, assay devices and assay methods of the present invention are useful both for the detection of a large number of dif...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More
Patent Type & Authority Applications(United States)
Login to View More