Methods of increasing the concentration of a nucleic acid

a nucleic acid and concentration technology, applied in the field of methods of increasing the concentration of nucleic acids, can solve the problems of difficult catalysis selection, serious shortcomings, and few attempts to improve the properties
US20080166772A1Inactive Publication Date: 2008-07-10HOLLINGER PHILLIP +2
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
HOLLINGER PHILLIP
Publication Date
2008-07-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

We describe a method of selecting an enzyme having replicase activity, the method comprising the steps of: (a) providing a pool of nucleic acids comprising members each encoding a replicase or a variant of the replicase; (b) subdividing the pool of nucleic acids into compartments, such that each compartment comprises a nucleic acid member of the pool together with the replicase or variant encoded by the nucleic acid member; (c) allowing nucleic acid replication to occur; and (d) detecting amplification of the nucleic acid member by the replicase. Methods for selecting agents capable of modulating replicase activity, and for selecting interacting polypeptides are also disclosed.
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Description

[0001] This application is a divisional of U.S. utility application Ser. No. 10 / 387,387, filed Mar. 13, 2003, which is a continuation-in-part of international application PCT / GB01 / 04108, filed Sep. 13, 2001, which claims the priority of each of Great Britain application GB 0022458.4, filed Sep. 13, 2000, U.S. provisional application 60 / 283,771, filed Apr. 13, 2001 and U.S. provisional application 60 / 285,501, filed Apr. 20, 2001. Each of these priority documents is expressly incorporated herein in its entirety, including tables and drawings.FIELD OF THE INVENTION

[0002] The present invention relates to methods for use in in vitro evolution of molecular libraries. In particular, the present invention relates to methods of selecting nucleic acids encoding gene products in which the nucleic acid and the activity of the encoded gene product are linked by compartmentalisation.BACKGROUND TO THE INVENTION

[0003] Evolution requires the generation of genetic diversity (diversity in nucleic acid) f...

Claims

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