Identification and isolation of transitional cell carcinoma stem cells

Abstract

Transitional cell carcinoma stem cells (TCCSC) are identified. The cells can be prospectively isolated or identified from primary tumor samples, and are shown to possess the unique properties of cancer stem cells in functional assays for cancer stem cell self-renewal and differentiation, and in cancer diagnosis.

Description

BACKGROUND OF THE INVENTION[0001]Bladder cancer is the second most common urological malignancy in the United States, which accounts for approximately 13,000 deaths and 61,000 new cases per year. Ninety percent of bladder malignancies are classified as transitional cell carcinomas (TCCs), which originate from the transitional urothelium that is composed of 3-6 layers thick of basal, intermediate and multinucleate umbrella cells. Seventy percent of tumors are low grade non-invasive papillary lesions at diagnosis, which commonly recur, only 15-20% of which progress into muscle invasive disease. On the other hand, ˜30% of TCCs are muscle invasive at diagnosis. Fifty percent of patients with invasive TCCs die from metastasis within 2 years, and the 5-year survival rate for metastatic bladder cancer is only 6%.[0002]It has long been observed that patient bladder tumors contain intratumoral heterogeneity, consisting of tumor cells with diverse histological morphologies and distinct biolog...

AI Technical Summary

Problems solved by technology

Another possibility is that most cancer cells lack the ability to form a new tumor such, that only the dissemination of rare cancer stem cells can lead to metastatic disease.

However, because most cells within a cancer have limited proliferative potential, an ability to shrink a tumor mainly reflects an ability to kill these cells.

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