RNA-SeqTranscriptome Analysis of Spirodela Dormancy Without Reproduction and Identification of Molecular Targets Useful for Improving Biomass Production for Industrial Applications

US20150159165A1Active Publication Date: 2015-06-11RUTGERS THE STATE UNIV
1 Cites 1 Cited by

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • RNA-SeqTranscriptome Analysis of Spirodela Dormancy Without Reproduction and Identification of Molecular Targets Useful for Improving Biomass Production for Industrial Applications
  • RNA-SeqTranscriptome Analysis of Spirodela Dormancy Without Reproduction and Identification of Molecular Targets Useful for Improving Biomass Production for Industrial Applications
  • RNA-SeqTranscriptome Analysis of Spirodela Dormancy Without Reproduction and Identification of Molecular Targets Useful for Improving Biomass Production for Industrial Applications

Examples

Experimental program
Comparison scheme
Effect test

example i

[0044]To further uncover the regulation of gene expression as the phase switches, we took advantage of RNA deep sequencing, and compared the transcriptome between fronds and developing turions. A more comprehensive understanding of the gene repertoire and its regulation during turion formation has great potential for industrial applications including the redirection of carbon flow into higher energy products.

Calibration and Selection of Tissue Samples

[0045]A comprehensive study for turion formation has been done using abscisic acid (ABA) induction[14, 15, 17, 28, 29]. Three days after ABA induction, the Spirodelaprimordium is committed to turion development, which cannot be reversed. All primary biosynthesis of protein, mRNA and DNA are shutdown resulting in the onset of the dormant state[28]. To calibrate our growing conditions with previous investigations, we used transmission electron microscopy (TEM) to investigate different developmental stages. We chose fronds and developing t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Contentaaaaaaaaaa
Login to View More

Abstract

Compositions and methods are provided for altering carbon partitioning in biomass isolated from Duckweed.

Description

[0001]This application claims priority to U.S. Provisional Application No. 61 / 912,328 filed Dec. 5, 2013, the entire disclosure being incorporated herein by reference.FIELD OF THE INVENTION[0002]This invention relates to the fields of plant molecular biology and recombinant manipulation of plant species in order to maximize production of biomass having desirable characteristics. More specifically, the invention provides valuable gene targets for manipulating carbon production in Duckweed based on results obtained from deep sequencing of the Duckweed genome.BACKGROUND OF THE INVENTION[0003]Several publications and patent documents are referenced in this application in order to more fully describe the state of the art to which this invention pertains. Full citations for these references are found within and at the end of the specification. The disclosure of each of these references is incorporated by reference.[0004]Plants, unlike animals, do not have fur nor can they seek shelter to ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
11 Jun 2015
Publication
US20150159165A1
IPC
C12N15/82
CPC
C12N15/8245; C12N15/8247; C12N15/8218
Inventors
MESSING, JOACHIM; WANG, WENQIN