2 results about "Mitogen activate protein" patented technology

The invention provides a protein composition for inhibiting inflammatory reaction, a plasmid and application thereof. The protein composition comprises listeriolysin O and mitogen activated protein kinase kinase 3; according to the construction method of the plasmid, an LLO gene, an NLRP3 gene and an MKK3 gene are taken as templates, and are respectively connected to an N-pCMV-Myc vector, an N-pCMV-HA vector and an N-pCMV-FLAG vector by using a homologous recombination method. The invention finds a new regulation channel for inhibiting inflammatory reaction when a host is infected by listeria monocytogenes, and finds that LLO can specifically bind MKK3 and NLRP3 by virtue of a bridge catalytic action, and then promotes degradation by virtue of a proteasome way, so that the inflammatory reaction of cells is inhibited. Under the discovery, the protein composition for inhibiting the inflammatory reaction, the plasmid and the construction method and application of the plasmid are prepared.

This invention discloses the application of compound 35C3 in inducing plant immunity and enhancing plant disease resistance, belonging to the field of biopesticide technology. Experiments have shown that compound 35C3 can effectively activate the phosphorylation of plant mitogen-activated protein kinase, induce reactive oxygen species (ROS) bursts, and upregulate the expression levels of disease resistance-related genes FRK1, PR1, PR2, WRKY29, and WRKY53. Pathogen inoculation experiments demonstrated that 35C3 can significantly enhance the resistance of Arabidopsis thaliana to *Pseudomonas syringae* and significantly enhance the resistance of poplar to *Colletotrichum gloeosporioides* and *Botrytis cinerea*. Its mechanism of action is to activate the plant's own immune system rather than directly inhibiting pathogen growth. Furthermore, 35C3 elicits immune responses in various crops, showing broad-spectrum applicability. This invention provides a new approach for developing environmentally friendly biopesticides and has significant agricultural application value.