The invention discloses a
lactate oxidase portable
mobile phone sensing method based on a composite MXene thin-layer material and an application of the
lactate oxidase portable
mobile phone sensing method. Firstly, a Cu0. 9Zn0. 1S (at) MXene thin-layer material is synthesized and can catalyze H2O2 into hydroxyl free radicals, the generated hydroxyl free radicals react with a colorless 3, 3 ', 5, 5'-
tetramethyl benzidine (TMB) solution to generate blue oxidation-state 3, 3 ', 5, 5'-
tetramethyl benzidine (oxTMB), the oxTMB can have an obvious
ultraviolet absorption peak at 652nm, and a novel visual
analysis method is constructed by taking the peak as
signal output; besides,
lactate oxidase can catalyze
lactic acid to generate
pyruvic acid and H2O2, a new method is used for LA or LOX analysis by taking H2O2 as a relation, H2O2 generated by the reaction can participate in the oxidation reaction of TMB, an obvious
ultraviolet absorption peak can appear at 652nm, and the inhibitor Fe (III) can inhibit the reaction. On the basis, a portable
mobile phone sensing method which is rapid, simple and low in cost is constructed, and the method is successfully applied to sensitive detection of LOX in HEK293S cells and has scientific significance.