The invention discloses traceless povidone iodine disinfectant which is prepared from, by weight, 4.0%-4.5% of polyvinylpyrrolidone, 0.45%-0.55% of iodine, 0.2%-0.8% of chelating agents, 0.2%-0.8% of stabilizer, 0.1%-0.2% of potassium hydroxide, 0.4%-0.8% of disodium hydrogen phosphate, 0.2%-0.8% of citric acid, 0.1%-0.5% of potassium iodide, 0.2%-0.5% of potassium iodate and the balance water. According to the traceless povidone iodine disinfectant, after iodide and polyvinylpyrrolidone are in complexation, a certain complexation and wrapping technology is adopted, the traceless povidone iodine disinfectant is cleaned from a floor with clean water within 24 hours, and nearly no yellow trace exists when observation is conducted with eyes. Compared with ordinary povidone iodine, namely iodophor, the traceless povidone iodine disinfectant has great advantages on the aspect of floor yellowing, so that the visual influence on operating personnel in an operating room is reduced, and the service life of the floor of the operating room is prolonged.