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Method for extracting lutein from chlorella cell

A technology of chlorella and lutein, which is applied in the field of biotechnology and can solve the problems of rare research reports

Inactive Publication Date: 2008-03-05
UNIV OF SCI & TECH BEIJING +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although scholars at home and abroad have done a lot of research on the optimal control method of extracting lutein from marigolds, there are few research reports on how to efficiently extract lutein from Chlorella

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  • Method for extracting lutein from chlorella cell

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Embodiment Construction

[0018] 1. Chlorella cell experiment material: The algal species used in the experiment of the present invention is a strain of Chlorella-USTB01 (Chlorella sp) that we screened and can grow heterotrophically without light, cgmcc No1448, date of preservation: August 25, 2005 day. After 5 days of heterotrophic batch culture, the algal cells were harvested by centrifugation (10000 rpm, 10 minutes) in a 50ml centrifuge tube, and after the supernatant was poured off, the algal cells settled at the bottom of the centrifuge tube were washed with 50 mmol phosphate The buffer solution (pH7.0) was resuspended, and the supernatant was discarded by centrifugation again. After washing the algal cells twice, the algal cells were harvested by centrifugation again, so that the dry weight of the algal cells remaining at the bottom of each centrifuge tube was 35 mg.

[0019] 2. Extract lutein from Chlorella cells: Add 5.0ml of methanol and n-octane to the centrifuge tube respectively, and then i...

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Abstract

This invention relates to a method for extracting lutein from Chlorella pyrenoidosa cells, belonging to the biotechnology field. It uses a mixed solvent of polar solvent methanol and non-polar solvent octane to extract lutein from Chlorella pyrenoidosa cells, wherein: centrifuge to collect the cultured Chlorella pyrenoidosa cells with 50-500ml centrifuge tubes, decant the supernatant and add in 5.0-50ml mixed solvent at carbon and oxygen atom ratio of 10:1-40:1, put the centrifuge tube on the ice and then disintegrate by ultrasonic, disintegrating time 10-30min, interval time 2-5sec between every 5-10sec ultrasonic, output power of the cell ultrasonic bucker 200-600W, after disintegration centrifuge at 5000-15000rpmat 4-8Deg C for 5-20min. It can obtain large amount of lutein extracted from the Chlorella pyrenoidosa cells from the extracting solvent supernatant octane phase.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular provides a method for extracting lutein from Chlorella cells, which uses a small molecular weight polar organic solvent methanol and a non-polar reagent n-octane to mix as the extraction method. The agent can efficiently extract lutein from chlorella cells, and has important application value and prospect in the production of important life active substance lutein by using chlorella. Background technique [0002] Lutein belongs to an asymmetric dihydroxy carotenoid and is the main component of the macular pigment in the retina of the human eye. Current studies have found that lutein mainly has the following three aspects of medical value: 1) preventing macular degeneration in the elderly; 2) having anti-cancer effects; 3) preventing and treating cardiovascular and cerebrovascular diseases. In addition, as a high-value pure natural pigment, lutein can be widely used in industries su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C403/24
Inventor 闫海李雅雯王素琴张宾赵晶晶陆钢张明忠
Owner UNIV OF SCI & TECH BEIJING