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Preparation method of scorpionfish-ink polysaccharide

A technology of ink and squid, applied in the field of preparation of squid ink polysaccharides, can solve the problems of difficult separation and extraction of squid ink polysaccharides, mixing, etc., achieve the effect of simple separation and extraction, and overcome thermal instability

Active Publication Date: 2009-03-11
宁波超星海洋生物制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, some fat and protein will also be mixed in the raw material of the ink sac as waste, which will increase the difficulty in the separation and extraction of squid ink polysaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: the formula of squid ink polysaccharide of the present invention is, and the weight ratio of each raw material is as follows:

[0013] Live frozen squid ink manually sorted 99.985 servings

[0014] 2000u / g food grade lipase 0.005 parts

[0015] 1 million u / g food grade papain 0.01 parts

[0016] Among them, 2000U / g food-grade lipase is a product produced by Shenzhen Green Microorganism Engineering Co., Ltd., and its international enzymatic number is EC 3.1.1.3; 1 million U / g food-grade papain is a product produced by Kunming Youshun Food Co., Ltd. Enzyme number is EC 3.4.22.2.

[0017] The preparation method of the squid ink polysaccharide of the present invention is: at first to the squid of living body freezing natural thawing, artificially separate ink sac, remove the outer envelope of ink sac, get its content ink (still in ice slag shape); Add the specified weight of squid ink and deionized water into the stainless steel reactor according to the wei...

Embodiment 2

[0018] Embodiment 2: the formula of squid ink polysaccharide is: the weight ratio of each raw material is as follows:

[0019] Live frozen squid ink manually sorted 99.984 servings

[0020] 2000u / g food grade lipase 0.008 parts

[0021] 1 million u / g papain 0.008 parts

[0022] The manufacturing method of embodiment 2 is the same as the manufacturing method of embodiment 1, but the precipitating agent of polysaccharide is changed into chemical pure acetone from 95% edible ethanol, to adapt to the convenient selection of precipitating agent of different pharmaceutical factories.

Embodiment 3

[0023] Embodiment 3: difference with embodiment 1 is that the formula of squid ink polysaccharide is: the weight ratio of each raw material is as follows:

[0024] 50 parts of live frozen squid ink sorted manually;

[0025] 2000u / g food grade lipase 0.005 parts;

[0026] 0.001 parts of 1 million u / g food grade papain.

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PUM

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Abstract

A squid ink polyose is prepared proportionally from the ink sac of squid, food-class lipase and food-class papain through proportional mixing, enzyme hydrolyzing, filtering, depositing in edible alcohol or chemical-purity acetone, dissolving deposit in deionized water, recovering alcohol or acetone, drying the deposit, and pulverizing. It has high stability.

Description

technical field [0001] The invention belongs to the field of marine biological medicine and antitumor medicine, and mainly relates to a preparation method of squid ink polysaccharide. Background technique [0002] In the past 30 years, due to the rapid development of cell biology and molecular biology, many biological functions of polysaccharides have been gradually revealed and understood. For example, research in life sciences in recent years has proved that polysaccharides participate in many important life processes of organisms in the form of sugar chains and other biomacromolecular covalently linked compounds such as glycoproteins and glycolipids. Glycoproteins and glycolipids are important components of cell membranes. As carriers and transmitters of biological information, they regulate cell growth, differentiation, metabolism, and immune responses. The resulting new discipline, glycogen biology (Glycobiology), provides precise microscopic descriptions for immunolog...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/48A61P35/00C12P19/04A61K31/715
Inventor 叶再府叶再镯章元炳
Owner 宁波超星海洋生物制品有限公司
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