Method of extracting undenatured natural collagen from tanning waste leather offcut

A technology of natural collagen and scraps, applied in the field of extracting undenatured natural collagen, can solve the problems of low added value and no biological activity, and achieve the effect of unique biocompatibility, low biodegradability and broad application prospects

Inactive Publication Date: 2009-08-12
柳州市宏升胶原蛋白肠衣有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] To sum up, the products extracted from the leather scrap waste produced in tanning are all hydrolyzed collagen, which has no biological activity and low added value

Method used

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  • Method of extracting undenatured natural collagen from tanning waste leather offcut
  • Method of extracting undenatured natural collagen from tanning waste leather offcut

Examples

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Effect test

Embodiment 1

[0027] Example 1: Take the tanned skin residue of pork slices, mince them, wash them three times with warm water at 30-35° C., take 100 g of debris, and add 2 g of fatty acid polyoxyethylene ether and 3 g of alkylphenol polyoxyethylene ether. The solution was soaked for 3 hours at a temperature of 32°C. After filtration, 10 g of isopropanol was added and shaken at room temperature for 24 hours, and then filtered. Adjust the pH to 2 with acid, add 2 g of pepsin, and stir for 24 hours at a temperature of 4-6°C. Remove the filter residue by centrifugation, add 0.7M NaCl to the supernatant for salting out, dissolve the precipitate with 0.5M acetic acid, repeat the salting out once, and then process through membrane dialysis to obtain a 5% undenatured natural pigskin collagen solution of about 600g.

Embodiment 2

[0028]Example 2: Take the tanned skin residue of pig slices, mince it, wash it 3 times with warm water at 30-35°C, take 100g of debris, and put it into a mixture made of 10g of fatty acid polyoxyethylene ether and 5g of alkylphenol polyoxyethylene ether. soaked in the solution for 24 hours at a temperature of 34° C., after filtering, add 50 g of acetone and shake at room temperature for 48 hours, and filter. Adjust the pH to 4 with acid, add 5 g of 537 protease, and stir for 48 hours at a temperature of 4-6°C. Remove the filter residue by centrifugation, add 2.1M NaCl to the supernatant for salting out, dissolve the precipitate with 1.0M acetic acid, repeat the salting out for 3 times, and then process through membrane dialysis to obtain an undenatured natural pigskin collagen solution with a concentration of about 8%. 600g.

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Abstract

A method for extracting undenatured natural collagen from tanning waste leather leftovers, which is characterized in that 100 parts of pig slice tanned leather leftovers are washed 3-5 times with 2-5 parts of water at 30-35° C. Soak in 5-15 parts of nonionic surfactant solution for 3-6 hours, repeat twice, filter, treat with 10-50 parts of organic solvent for 12-24 hours, and filter to obtain sediment; take 100 parts of the above sediment, add protease 0.5- 6 parts, adjusted pH=2-4 with acid, stirred at 4-6°C for 1-3 days, centrifuged to obtain collagen solution, salted out with 0.5-3M NaCl, precipitated after centrifuged, dissolved with 0.1-2M acetic acid and treated repeatedly Twice, through membrane dialysis to obtain 5-8% undenatured natural pigskin collagen solution.

Description

technical field [0001] The invention relates to a method for extracting undenatured natural collagen from tanning waste leather leftovers, belonging to the field of high value-added transformation of tanning solid waste. Background technique [0002] According to incomplete statistics, my country processes 170 million pieces of leather such as pigs and cattle every year, and produces about 700,000 tons of scrap waste (without chromium). Among them, it is mainly raw leather trimming scraps, scraps of gray leather or scraps produced by tanned leather. These solid wastes are one of the important factors causing serious pollution in the leather industry. Since their main component is collagen, using leather leftovers to extract high-quality collagen and develop it into high-value-added products can not only provide a way for the leather industry to solve solid waste pollution, but more importantly, animal matter resources The comprehensive development and high-value utilizatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09H3/00
Inventor 李国英赵帅李东李洋
Owner 柳州市宏升胶原蛋白肠衣有限公司
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