Termite resisting method utilizing fermented solution of tree endophyte to inhibit symbiotic bacteria of termite
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A technology of endophyte and fermentation broth, applied in the field of anti-termite, can solve problems such as environmental pollution
Inactive Publication Date: 2009-09-23
HEILONGJIANG UNIV
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Problems solved by technology
[0002] Existing medicaments for killing termites are all chemical medicaments, so they all have great toxic and side effects, causing serious environmental pollution
Method used
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specific Embodiment approach 1
[0004] Specific embodiment one: this embodiment utilizes the metabolites of endophytic bacteria in trees to inhibit termite symbiotic bacteria so as to realize the anti-termite method: (1) separate symbiotic bacteria with lignin and / or cellulose decomposition ability from termites as indicator bacteria; (2) isolate tree endophytes from termite-resistant trees, and ferment them to obtain tree endophyte metabolites; (3) use Oxford cup method to inhibit termite symbiotic bacteria by using tree endophyte metabolites Bacteria test; (4) Soak the filter paper sheet with metabolites of tree endophytic bacteria that have an inhibitory effect on the symbiotic bacteria of termites, and carry out a feeding test on termites. The termite-resistant trees are Western Red Cedar and port-orford-cedar.
specific Embodiment approach 2
[0005] Specific embodiment two: the present embodiment utilizes the metabolites of tree endophytes to suppress termite symbiotic bacteria so as to realize the anti-termite method:
[0006] 1. Selection of materials:
[0007] 1. Western Red Cedar (sampled in Louisiana, USA on September 10, 2003)
[0008] 2. port-orford-cedar (sampled in Oregon, USA in September 2003)
[0009] 3. Black-winged soil termite (Odontotermes formosanus) (sampled from Mount Luojia, Wuhan, China)
[0010] 4. American termites (sampling from Louisiana State University, USA)
[0011] Second, the preparation of culture medium:
[0012] (1) Indicator culture medium:
[0013] 1. Cellulose medium:
[0014] 1.1 Bacterial cellulose medium:
[0015] CMC-Na (sodium carboxymethylcellulose referred to as CMC) 10g, NH 4 h 2 PO 4 0.5g, K 2 HPO 4 2.0g, MgSO 4 2.0g, MnSO 4 ·H 2 O 2.5mg, FeSO 4 ·7H 2 O 7.5mg, NaCl 0.3g, peptone 10g, agar 20g, water 1000ml.
[0016] 1.2 Fungal cellulose medium:
[001...
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Abstract
The invention relates to an anti-termite method for inhibiting termite symbiotic bacteria by using a fermentation liquid of endophytic bacteria in trees, and relates to an anti-termite method for inhibiting termite symbiotic bacteria by using a fermentation liquid of endophytic bacteria in trees. The present invention is carried out as follows: from termites, symbiotic bacteria with lignin and / or cellulose decomposition ability are isolated as indicator bacteria; endophytic bacteria are isolated from termite-resistant trees, and fermented to obtain endophytic bacteria fermentation bacteriostatic test on termite symbiotic bacteria by using the fermentation liquid of endophytic bacteria in trees; the feeding test on termites was carried out by soaking the filter paper with the fermentation liquid of endophytic bacteria in trees which has an inhibitory effect on the symbiotic bacteria of termites. After statistical analysis, it is shown that the termites in the test group eat significantly less filter paper than the control group, and the death rate of the termites in the test group is significantly higher than that of the control group, and the difference is extremely significant; The control group was weak, indicating that the fermentation broth contained compounds that were resistant to termites.
Description
Technical field: [0001] The invention relates to a method for isolating endophytes, in particular to an anti-termite method for inhibiting termite symbiotic bacteria by utilizing the fermentation liquid of tree endophytes. Background technique: [0002] Existing medicaments for killing termites are all chemical medicaments, so they all have very large toxic and side effects and cause serious environmental pollution. Invention content: [0003] The object of the present invention is to provide an anti-termite method using the fermentation liquid of endophytic bacteria in trees to inhibit termite symbiotic bacteria, and the method uses microorganisms to produce non-toxic or low-toxic termite-resistant compounds to humans and animals. The present invention utilizes the fermented liquid of tree endophytic bacteria to suppress termite symbiotic bacteria so as to realize the anti-termite method: (1) isolate symbiotic bacteria with lignin and / or cellulose decomposition ability fr...
Claims
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Application Information
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