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Method of preparing lycopene from trispore Bruce mould

A technology of Blakeslea and Blakeslea trispora, which is applied in the fields of chemical industry, food, and medicine. It can solve the problems of limited oxygen supply, high medium viscosity, and increased operating costs, so as to achieve fast oxygen transfer and increase biomass. , The effect of less bubble generation

Inactive Publication Date: 2009-10-21
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the method of using Blakeslea trispora to produce lycopene, since Blakeslea trispora is a highly aerobic and viscophilic microorganism, the medium used has a high viscosity and a high solid content, and also contains 5% vegetable oil (providing double for metabolism). key, resulting in a low dissolved oxygen rate in the fermentation system); during the growth process, it is easy to tangle into agglomerates, resulting in a far insufficient amount of dissolved oxygen in the logarithmic growth period, so the problem of oxygen supply is to restrict the biological reaction in the fermentation process of B. trispora and important factors of life process (Jeong JC.Lee IY.Kim SW.Park YH.1999.Biotechnol.Lett.21:683-686; Kim SW.SeoWr.Park YH.1997.J.Ferment.Bioeng.84:330 -332)
[0006] For most fermentations, the lack of oxygen will cause metabolic abnormalities, biomass and target metabolite yield reduction; and because the solubility of oxygen in aqueous solution is very low (its saturation is less than 7mg / L), the oxygen supply in fermentation is very low It is easy to become the main contradiction, so how to ensure the supply of oxygen in fermentation to meet the oxygen demand of production strains is one of the keys to improve fermentation production capacity and reduce costs
At present, the traditional mechanical oxygen supply methods such as stirring ventilation and shaking table oscillation have the following problems: when the operating conditions of the equipment are reduced, the oxygen supply is severely limited, and the formation of target metabolites is blocked; when the operating conditions are increased, the high mechanical strength will be enhanced The damage to the cells caused by shearing will cause the bacteria to be damaged and cannot metabolize normally, and the operating cost will also increase greatly; while improving the design of bioreactors, stirring paddles and air distributors, and using oxygen-enriched air will reduce production costs. greatly improved, so it is necessary to find a new way to strengthen oxygen supply

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Seed cultivation:

[0022] The B. trispora ATCC 14271(+) and ATCC 14272(-) strains were inoculated into the seed medium. The components and content of the seed medium are: starch 40g / L, corn steep liquor 50g / L, KH 2 PO 4 1g / L, MgSO 4 0.1g / L, VB 1 0.01g / L, pH6.5. 28°C, 180rpm shaker culture for 40h, (+)(-) culture solution obtained by mixing 1:2 to obtain seed solution.

[0023] (2) Fermentation culture:

[0024] Inoculate the seed liquid into the fermentation medium according to the 10% inoculum. The components and content of the fermentation medium are: starch 40g / L, soybean meal 20g / L, corn steep liquor 25g / L, KH 2 PO 4 1g / L, MgSO 4 0.1g / L, VB 1 0.01g / L, 20ml / L filter-sterilized n-hexane, pH 6.5. Fermentation culture uses a 500ml Erlenmeyer flask with a liquid volume of 100ml, cultured at 26°C, 220rpm shaker for 4 days, and then harvested the bacteria. After fermentation for 46 hours, 1.5g of pyridine or tertiary amine compound blocker was added.

[0025] (3) Extracti...

Embodiment 2

[0028] (1) Seed cultivation:

[0029] The B. trispora ATCC 14271(+) and ATCC 14272(-) strains were inoculated into the seed medium. The components and content of the seed medium are: starch 500g / L, corn steep liquor 40g / L, KH 2 PO 4 1.5g / L, MgSO 4 0.2g / L, VB 1 0.02g / L, pH6.5. 26°C, 200rpm shaker culture for 38h, (+)(-) culture solution obtained by mixing 1:2 to obtain seed solution.

[0030] (2) Fermentation culture:

[0031] The components and content of the fermentation medium are: starch 40g / L, soybean meal 20g / L, corn steep liquor 25g / L, KH 2 PO 4 1g / L, MgSO 4 0.1g / L, VB 1 0.01g / L, 15ml / L filter-sterilized n-dodecane, cultured at 30°C, 230rpm shaker for 6 days, and harvested the cells. 1.5 g of pyridine or tertiary amine compound blocker was added after fermentation to 50 hours, and the others were the same as in Example 1.

[0032] (3) Extraction

[0033] The fermentation broth was filtered with gauze to obtain wet bacteria, and the wet bacteria were placed in a vacuum drying...

Embodiment 3

[0035] (1) Seed cultivation:

[0036] The seed culture process is the same as in Example 1.

[0037] (2) Fermentation culture:

[0038] The seed liquid was inoculated into the fermentation medium according to the 10% inoculum. The components and content of the fermentation medium are: starch 50g / L, soybean meal 25g / L, corn steep liquor 20g / L, KH 2 PO 4 1.5g / L, MgSO 4 0.05g / L, VB 1 0.01g / L, tween-801g / L, 10ml / L filter-sterilized n-hexane, pH 6.5. The cells were harvested after culturing on a shaker at 28°C and 250 rpm for 5 days. 1.5 g of pyridine or tertiary amine compound blocker was added after fermentation to 48 hours, and the others were the same as in Example 1.

[0039] (3) Extraction:

[0040] The fermentation broth was filtered with gauze to obtain wet bacteria, and the wet bacteria were placed in a vacuum drying box at 50°C and vacuum dried for 48 hours to obtain 5.08 g of dry bacteria powder. The dried bacterial powder was crushed and extracted with petroleum ether (take...

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Abstract

The invention relates to a method for preparing lycopene by fermenting the three spora boulardii. By adding a suitable oxygen carrier to the fermentation medium, an oxygen carrier fermentation system is provided to increase the dissolved oxygen concentration in the medium without additional energy consumption, thereby increasing the biomass of cells and lycopene in cells content, greatly increasing the yield of lycopene.

Description

Technical field: [0001] The invention relates to a method for preparing lycopene by fermentation with B. trispora. It belongs to the fields of food, medicine and chemical industry. Background technique: [0002] Lycopene is a fat-soluble carotenoid, which is an intermediate in the biosynthesis of many carotenoids. Compared with β-carotene, although lycopene is not a precursor of vitamin A, its ability to eliminate singlet oxygen and free radicals and its antioxidant effect are better than β-carotene. It has anti-cancer, anti-cancer, and prevention effects. A series of important physiological functions such as cardiovascular disease. [0003] Lycopene is a straight-chain hydrocarbon composed of 11 conjugated and 2 non-conjugated carbon-carbon double bonds. It has the characteristics of easy oxidation and easy isomerization, so it is difficult to extract. At present, the commonly used extraction methods are: organic solvent extraction, supercritical extraction, and saponification. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P5/02C12R1/645
Inventor 袁其朋徐芳朱艳
Owner BEIJING UNIV OF CHEM TECH
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