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Liquid stablilized serum cholinesterase reagent

A serum cholinesterase and reagent technology, applied in the field of quantitative determination of serum cholinesterase, can solve problems such as reconstitution or reaction system deviation from the optimal reaction pH value of cholinesterase

Active Publication Date: 2009-11-04
DIRUI MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a liquid stable serum cholinesterase reagent to solve the problem of redissolving or reaction system deviating from the optimal reaction pH value of cholinesterase in the application of cholinesterase reagent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: The pH of the working reagent is 7.70, and the substrate concentration of reagent 2 is 15 mM.

[0033] Reagent 1:

[0034] Reagent Component Concentration

[0035] Phosphate buffer pH8.1 31.25mM

[0036] Phenol 2mM

[0037] Reagent 2:

[0038] Reagent Component Concentration

[0039] Acetic acid buffer pH4.75 20mM

[0040] Phenol 3mM

[0041] 5,5′-dithiobis(2-nitrobenzoic acid) 1.31mM

[0042] Butyrylthiocholine 15mM

[0043] Reagent 1 and reagent 2 were placed at 37°C for 14 days, the substrate concentration, sulfhydryl chromogenic reagent and reagent performance did not drop significantly, and the accuracy and precision of reagent determination were not affected in any way. The number of factors used in the calculation of reagent determination is between 7000 and 8000.

Embodiment 2

[0044] Example 2: The pH of the working reagent is 7.60, the substrate of reagent 2 is propionylthiocholine, and the concentration is 25 mM.

[0045] Reagent 1:

[0046] Reagent Component Concentration

[0047] Phosphate buffer pH8.0 31.25mM

[0048] Phenol 2mM

[0049] Reagent 2:

[0050] Reagent Component Concentration

[0051] Acetic acid buffer pH4.5 20mM

[0052] Phenol 5mM

[0053] 5,5′-dithiobis(2-nitrobenzoic acid) 1.31mM

[0054] Propionylthiocholine 25mM

[0055] Reagent 1 and reagent 2 were placed at 37°C for 14 days, the substrate concentration, sulfhydryl chromogenic reagent and reagent performance did not drop significantly, and the accuracy and precision of reagent determination were not affected in any way. The number of factors used for reagent determination and calculation is 7000-8000.

Embodiment 3

[0056] Embodiment 3: Reagent 1 is Tris buffer solution, working reagent pH7.60~7.80, reagent 2 is identical with embodiment 1:

[0057] Reagent Component Concentration

[0058] Tris buffer pH7.80 35mM

[0059] Phenol 2mM

[0060] Reagent 1 and reagent 2 were placed at 37°C for 14 days, the substrate concentration, sulfhydryl chromogenic reagent and reagent performance did not drop significantly, and the accuracy and precision of reagent determination were not affected in any way. The number of factors used for reagent determination and calculation is 7000-8000.

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PUM

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Abstract

The invention relates to a liquid-stable serum cholinesterase reagent, which is used for quantitatively measuring the activity of cholinesterase in human serum or other body fluids in vitro. Composed of reagent 1 and reagent 2, by adding a non-reactive stabilizer to the reagent, the effective reaction concentration and effective reagent performance of butyrylthiocholine or propionylthiocholine and dithiobisnitrobenzoic acid are maintained , so as to achieve in the process of preparation, production and subsequent application, the preparation technology adopted in the present invention and the assay reagent prepared according to this technology are all ready-to-use liquids, and the optimum pH value for reaction measurement is between 7.40~7.80.

Description

technical field [0001] The invention relates to a reagent for measuring serum cholinesterase and a preparation method thereof, which are used for quantitative determination of serum cholinesterase. Background technique [0002] Serum cholinesterase is derived from the liver. In acute and chronic hepatitis, liver cirrhosis, liver cancer, hypoproteinemia, etc., the activity decreases, indicating that the function of liver synthetase is impaired. Many organic compounds inhibit the activity of cholinesterase, which is commonly seen in organophosphate poisoning clinically. In mild cases, the activity of cholinesterase can be reduced by 30%; in moderate cases, the activity can be reduced by 50%; in severe cases, the activity The drop can be up to 70%. Increased cholinesterase activity is common in nephrotic syndrome, hyperthyroidism, diabetes and other diseases. The determination method of serum cholinesterase includes the method recommended by the German Society of Clinical Ch...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78C12Q1/46
Inventor 王学忠顾小丰
Owner DIRUI MEDICAL TECH CO LTD
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