Chinese medicinal soft capsule effective ingredient detection method
A technology of active ingredients and detection methods, applied in the drug composition of traditional Chinese medicine soft capsules, and in the field of drug composition of Jinshuibao soft capsules, can solve problems such as undiscovered and labor-intensive
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Embodiment 1
[0036] Prescription: fermented Cordyceps powder 1.8kg, polyethylene glycol-400 2.4kg, propylene glycol 0.35kg
[0037] Preparation method: Take the fermented Cordyceps fungus powder, add propylene glycol and polyethylene glycol-400, pass through a colloid mill to grind for 20 minutes, and press it into soft capsules on a soft capsule machine.
Embodiment 2
[0039] Prescription: fermented Cordyceps powder 2.2kg, polyethylene glycol-400 2.8kg, propylene glycol 0.45kg, ethylparaben 0.1kg
[0040] Preparation method: Take the fermented Cordyceps fungus powder, add propylene glycol, polyethylene glycol-400 and ethylparaben, pass through a colloid mill to grind for 20 minutes, and press into soft capsules on a soft capsule machine.
Embodiment 3
[0042] Prescription: fermented Cordyceps powder 2kg, polyethylene glycol-400 2.6kg, propylene glycol 0.4kg
[0043] Preparation method: take fermented Cordyceps fungus powder, add propylene glycol and polyethylene glycol-400, mix well, and make 10,000 soft capsules
[0044] Quality Standard:
[0045]Identification (1) Take adenine reference substance and adenosine reference substance, add dilute ethanol to make a solution containing 10ug per 1ml, respectively, as the reference substance solution. Test according to high performance liquid chromatography (Chinese Pharmacopoeia, an appendix VID of the 2000 edition), using octadecylsilane bonded silica gel as a filler, and using 0.5% tetrahydrofuran in a phosphate buffer solution [0.066mol / L potassium dihydrogen phosphate solution- 0.066mol / L disodium hydrogen phosphate solution (4: 6)] is mobile phase, and detection wavelength is 260nm, absorbs each 20ul of need testing solution and above-mentioned reference substance solution u...
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