Laser-induction fluorescence co-focusing scanning device and method

Abstract

The laser-induced fluorescence confocal scanning device and method belongs to the biochip detection technology field. The fluorescence collection and usage efficiency of existing devices is low, and meanwhile the focusing control in the scanning process is very difficult. The invention uses the middle hole full reflection mirror to optical divide the incident laser and the induced reflected fluorescence, and when a laser beam passing through the aperture, the energy loss is small, almost all laser energy can be irradiated to the biochip, and when collecting the fluorescence, the fluorescence collection angle is big, not only improving the efficiency of laser incident, but also improving the fluorescence collection efficiency. Using computer control, it can maintain approximately confocal relation between the light source pinhole and the detector pinhole, and only need focus one time before scanning, making the focusing control easy and convenient.

Description

technical field [0001] The invention discloses a laser-induced fluorescence confocal scanning device, and the laser-induced fluorescence confocal scanning device and method belong to the technical field of biochip detection. Background technique [0002] As a new high-tech product, biochips are being widely used in life sciences, medical research and applications. The concept of biochips is derived from computer chips. In a narrow sense, biochips refer to microarrays of high-density DNA, proteins, cells and other biologically active substances coated on solid carriers such as silicon wafers, glass, plastics, and nylon membranes, mainly including cDNA microarrays, oligonucleotides, etc. Microarrays and protein microarrays. These microarrays are formed by orderly immobilizing biologically active substances on solid phase supports in the form of lattices. The biochemical reaction is carried out under certain conditions, and the reaction results are displayed by chemical fluor...

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Problems solved by technology

[0005] In the above-mentioned device, a semi-reflective lens is used to split the laser and the fluorescence induced by the laser. The collection and utilization efficiency of the fluorescence is not high. The incident laser has about 50% energy loss when splitting the light, and the fluorescence collected by the lens also has about 50% energy loss. There is a 50% energy loss

If there are multiple lasers with different wavelengths, different wavelengths of fluorescence will be induced, and the efficiency of fluorescence collection and utilization will be lower; at the same time, the pinhole of the light source and the pinhole of the detector maintain a strict confocal relationship, and it is very difficult to control the focus during scanning.

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