Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for extracting natamycin from fermentation technique culture

A technology of natamycin and culture, applied in the field of direct extraction of natamycin, can solve the problems of high production cost, long process and high loss of organic solvent

Active Publication Date: 2010-05-19
SHANDONG LUKANG PHARMA
View PDF11 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a method for extracting natamycin from fermentation technology culture, which is characterized by extracting natamycin from fermentation broth by non-organic solvent method, and solves the problem of extracting natamycin by organic solvent method. In the production process of the natamycin extraction process, the organic solvent loss is high and the production cost is high due to the long process. An economical, green and environmentally friendly extraction method suitable for industrial production is provided.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting natamycin from fermentation technique culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Take 3.0L of natamycin fermented liquid, content 4.6g / l. First cool down the fermentation broth to 15°C; then adjust the pH to 12.0 with 4mol / L KOH solution and stir for 30 minutes, keeping the temperature at 15°C during the process; centrifuge the alkalized fermentation broth at 3000 rpm for 15 minutes, collect Centrifuge the supernatant, and then adjust the pH of the supernatant to 6.0-7.0 with 1.0 mol / L HCl solution to precipitate natamycin. The crystal solution was suction-filtered, washed twice with an appropriate amount of anhydrous brine, and air-dried at 60° C. for 3 hours to obtain 11.90 g of natamycin, with a content of 84.5%.

Embodiment 2

[0028] 200 grams of natamycin mycelium, wherein the natamycin content is 8.9%, was added into 2 liters of anhydrous brine and stirred to fully suspend the mycelium, and the temperature was lowered to 5°C. Adjust the pH to 11.5 with 2 mol / L ammonia water and stir for 30 minutes. During the process, keep the temperature at 15°C, separate the bacterial residue by suction filtration, and collect the filtrate. Then use 0.5mol / L H 2 SO 4 The solution adjusts the pH of the filtrate to 6.0-7.0 to precipitate natamycin. The crystal solution was suction filtered and washed twice with an appropriate amount of anhydrous brine, then vacuum-dried at 50°C and -0.09MPa for 3 hours to obtain 15.0 g of natamycin with a content of 85.3%.

Embodiment 3

[0030] Get 1000L of natamycin fermented liquid, content 3.8g / l. First cool down the fermentation broth to 20°C; then adjust the pH to 12.0 with 4mol / L NaOH solution and stir for 30 minutes, keeping the temperature at 15°C; add 2Kg filter aid to the alkalized fermentation broth and stir evenly, filter to filter. Collect the filtrate, and then adjust the pH of the filtrate to 6.0-7.0 with 1.0 mol / L HCl solution to precipitate natamycin. The precipitate was separated with a three-legged centrifuge, washed twice with an appropriate amount of anhydrous brine, and then air-dried at 60° C. for 3 hours to obtain 3136 g of natamycin, with a content of 82.4%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method to extract myprozine from ferment technique culture, which comprises the following steps: adjusting the pH value of the culture to 10. 0-14. 0 with basic substance under the condition of 0-35 deg. c; stirring evenly; separating solid and liquid; collecting filter liquor; getting aqueous phase extract of myprozine; acidifying at normal temperature; adjusting the pHvalue of the filter liquor to 5. 0-9. 0; depositing myprozine; getting crystalline liquid of myprozine; proceeding solid-liquid separation; getting the product. This method possesses the advantages oflow cost, environmental protection and low demand for zymotic fluid and device.

Description

Technical field: [0001] The invention relates to a method for directly extracting natamycin from fermentation technology culture. Background technique: [0002] Natamycin is a polyene macrolide antibiotic; molecular formula C 33 h 47 NO 13 ; Molecular weight 665.75 Daltons; Natamycin is an amphoteric compound with an isoelectric point of pH6.5, and its structure generally has two configurations, namely enol and ketone structures. The structural formula of natamycin is: [0003] [0004] Because natamycin can effectively and specifically inhibit yeast and mold and has good physical and chemical stability and extremely low toxicity, natamycin is used in medical (external use), food, feed, and grain storage. Among them, especially in the application of food raw material preservation and food preservation, it has shown a good prospect. However, due to its extremely high production cost, the commercial application of natamycin products is greatly restricted, and it is onl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/08C12P19/62C12R1/465
Inventor 梁景乐张利强
Owner SHANDONG LUKANG PHARMA
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com