Megacine and its use

A technology of Bacillus megaterium and strains, applied to a strain of Bacillus megaterium and its application fields, can solve the problems of limited degradation ability and inapplicability of high-concentration toluene, and achieve the effect of improving processing efficiency and saving processing time

Active Publication Date: 2007-11-21
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] According to the records of the prior art, existing microorganisms have limited ability to deg

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1, the isolation and cultivation of B.megaterium J1 bacterial strain

[0018] 1. Isolation of strains

[0019] Sludge samples were collected at the sewage treatment plant of Fushun Petroleum No. 3 Plant. Add 1 drop of sludge aqueous solution to the screening medium, add 0.1g of toluene, spread it evenly with a spreader, place it in an incubator, and cultivate it at 30°C for 2 days; then use an inoculation loop to transfer the screened strains to In the LB medium, culture in an incubator at 30°C for 1 day, and the obtained strain is B. megaterium J1 strain.

[0020] The components of the screening medium are:

[0021] (NH 4 ) 2 SO 4 5g; K 2 HPO 4 1g;

[0022] K H 2 PO 4 4g; NaCl 2g;

[0023] MgSO 4 ·7H 2 O 1g; Agar 15g;

[0024] Water 1000mL; pH 7.0.

[0025] 2. Cultivation of B. megaterium J1 strain

[0026] Use an inoculation loop to inoculate the strains screened in shake flasks with a liquid volume of 50 mL, and culture...

Embodiment 2

[0032] The cultivation of the B. megaterium J1 strain was the same as in Example 1. Take 2 shake flasks, add 50mL sterile water and 1.5mL toluene respectively. One of the shake flasks was taken as a blank control group, and the cultured J1 bacterial agent was added to the other shake flask to make its OD value 5.0. The two shaker flasks were placed on a shaker to vibrate for reaction, and the conditions of the shaker were: temperature 30° C., rotation speed 150 rpm. After 10 hours, samples were taken and analyzed, and the results are shown in Table 2.

[0033] initial

Embodiment 3

[0035] The cultivation of the B. megaterium J1 strain was the same as in Example 1. Take 2 shake flasks, add 50mL sterile water respectively, and then add 4g soil contaminated by 1.5mL toluene. One of the shake flasks was taken as a blank control group; the cultured B. megaterium J1 bacterial agent was added to the other shake flask to make its OD value 5.0. The two shaker flasks were placed on a shaker to vibrate for reaction, and the conditions of the shaker were: temperature 30° C.; rotation speed 150 rpm. After 10 hours, samples were taken for analysis, and the results are listed in Table 3.

[0036] initial

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Abstract

The Bacillus megaterium J1 is prepared by separating out from active sludge in sewage disposal tank of petrochemical plant, purifying and culturing. The preservative number is CGMCC No. 1487. It has excellent efficient degradable performance for toluene and can be used to dispose waste water and waste gas containing toluene, polluted water and soil.

Description

technical field [0001] The invention relates to a strain of bacillus megaterium and its application in controlling toluene pollution. Background technique [0002] Spills and leaks of petroleum and various fuel oils, as well as leakage of underground oil storage facilities have caused groundwater to be polluted by aromatic hydrocarbons, which has become very serious worldwide. Among them, monocyclic aromatic hydrocarbon pollutants such as benzene and toluene are the most concerned. They are the most water-soluble compounds in petroleum and various fuel oils, and constitute the most common organic pollutants in groundwater. Moreover, benzene and toluene belong to volatile organic compounds, which have become a large class of air pollutants second only to particle pollutants, which seriously pollute the atmosphere and are extremely harmful. With the rapid development of organic synthesis industry and petrochemical industry, the use of such organic compounds is increasing, esp...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B01D53/84B09C1/10C12R1/11
CPCY02A50/20
Inventor 范峰佟明友黎元生许谦
Owner CHINA PETROLEUM & CHEM CORP
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