Method for improving iris lactea seed germination rate

A germination rate and seed technology, applied in the field of garden plants, can solve the problems of human hazards, high cost and complicated operation in the surrounding environment, and achieve the effects of improving the germination rate, easy control and simple operation.

Inactive Publication Date: 2008-01-09

毕慧滨

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Problems solved by technology

[0014] In order to break the dormancy of Iris seeds and increase the germination rate, the existing methods mainly include low-temperature stratified sand storage, soaking Iris seeds with hormones, burning seeds with concentrated sulfuric acid, an...

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Method used

As can be seen from the data results of analysis table 5, after low-temperature stratification processing, the seeds of Rhizoma rhizome are germinated under different temperature-changing conditions of day and night respectively, and with respect to processing with constant temperature conditions, the germination rate of Rhizoma rhizome seeds improves to some extent , there was no significant difference in germination rate between the groups under diurnal temperature change (P>0.05). The results of the data in Table 7 show that the germination rate of the seeds of Rhizoma rhizome is improved to a certain extent after being treated with low temperature stratification and treated under the conditions of diurnal and night temperature changes. But on the whole, the combination of low temperature stratification treatment and diurnal temperature change has no obvious synergistic or interactive effect on improving the germination rate of S. pilosula seeds.

The data result...

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Abstract

The present invention discloses a method for raising germination percentage of sweet iris seeds. Said method includes the following steps: firstly, soaking sweet iris seeds in auxin, gibberellin or potassium nitrate solution, then taking out the sweet iris seeds from said solution and placing the sweet iris seeds into a day-and-night temperature variable and dark environment to make germination. At day, it uses 2.5-30deg.C temperature to make treatment for 6-12hr, and at night it uses 15-20deg.C temperature to make treatment for 12-18hr.

Application Domain

BiocidePlant growth regulators +2

Technology Topic

GerminationIris lactea +5

Examples

Effect test(9)

Test Example

[0032] The viability determination of test example 1 Iris seed

[0033] 1. Measurement method

[0034] Viability was measured by tetrazolium staining method. Take 100 seeds of Ma Lin, and repeat in 2 groups. After rinsing with tap water, soak in distilled water at 20°C for 48 hours; peel off the seed coat, and cut the seed longitudinally along the center of the embryo with a single-sided blade, keeping the cut surface unbroken; Soak the seeds in the tetrazolium solution for 18 hours, rinse the medicinal solution with clean water, take out the seeds and observe the dyeing of the embryos under a magnifying glass to distinguish the colored and uncolored seeds. Refer to the "International Seed Inspection Regulations" and "Tetrazolium Determination Manual" for identification and record. The number of viable seeds.

[0035] 2. Test results

[0036] The experimental results are shown in Table 1.

[0037] Table 1 The results of the determination of the viability of the seeds of Iris japonica in each period (unit: %)

[0038] name

[0039] The data in Table 1 shows that the vitality of the seeds of Iris is low, the highest is only 75.5%. Through the viability test of the seeds of Iris for two consecutive years, it is known that the viability of the seeds of Iris differs significantly in each period (F>F 0.01 ). The viability of the seeds of Ma Lin was low when it was newly collected, but the viability of the seeds after storage at room temperature for 2 years was only 60.5%, a drop of 15 percentage points.

Test Example

[0040] Test example 2 Nude embryo development test of Sinus chinensis seeds

[0041] 1. Test method

[0042] Select 50 mature and plump seeds of Iris chinensis, remove the skins and soak in water for 48 hours, cut the endosperm longitudinally with a blade to expose the seed embryo, and peel off the seed embryo with a dissecting needle. The seed embryo has a part of endosperm (accounting for about 1/5 of the total endosperm), so as not to affect the normal development of the seed embryo in the early stage: take a clean petri dish, put a double-layer filter paper inside, and place the seed embryo on it. Add distilled water to the petri dish to the extent of submerging the seed embryos, so that the seed embryos can fully absorb water and swell; place the petri dish in a 22°C incubator and cultivate under dark conditions, and observe the development of the seed embryos every day. 2. Test results

[0043] Different parts of the seed embryo coating (seed coat, endosperm) of Sinus cuspidatum have different effects on the formation of seed dormancy. In order to further study this effect, the intact seeds were used as controls, and the isolated embryos of the seeds were stripped of the endosperm. Germination test. With the gradual removal of the germ covering, the germination rate of the seeds increased significantly. It shows that the seed coat and endosperm both play a certain role in maintaining seed dormancy.

[0044] The test results showed that the development of the seed embryo was not obvious within 40 days, and basically remained at 1.90-2.10mm when the endosperm was coated. After releasing the endosperm, the embryo grew rapidly and reached 3.00mm in 7 days. The test results showed that the endosperm of Spermia japonica seeds had a strong mechanical effect, which seriously hindered the growth and development of the seed milk.

Test Example

[0045] Test Example 3 Germination Test of Iris Seeds

[0046] 1. Test method

[0047] The germination test adopts the conventional paper bed germination test method, as follows

[0048] 1. Germination test under constant temperature conditions: Soak the seeds in warm water at 40°C for 56 hours before the experiment, place 2 layers of filter paper in a petri dish with a diameter of 12 mm, soak the paper with water, pour off the excess water, and keep the moisture in the water. , select 50 seeds of submerged seeds and put them evenly on the paper in the petri dish, respectively at 15 ℃, 18 ℃, 20 ℃, 22 ℃, 25 ℃, 28 ℃, 30 ℃ constant temperature (LRH-250-GII microcomputer control Under the conditions of light incubator), observe the germination status of seeds within 90 days; set up a treatment group for each temperature, use 50 seeds for each treatment group, repeat 4 times, all treatments are carried out under dark conditions, to grow 1mm embryos The root is germination, and the number of germination is counted every day;

[0049] 2. Treatment under the condition of diurnal temperature change:

[0050] Soak the seeds in warm water at 40°C for 56 hours before the experiment, place a layer of filter paper in a petri dish with a diameter of 12 mm, soak the paper with water, pour off the rest of the water, keep the moisture in the degree of no water accumulation, and select the submerged seeds for each Put 50 grains on the paper in the petri dish, put them into the incubator (LRH-250-GII microcomputer-controlled light incubator) respectively under the following variable temperature conditions and carry out the germination test:

[0051] (1) Treatment at 25°C during the day (8:00-16:00, 8 hours in total), and 10°C at night (16:00--8:00, 16 hours in total) (group I);

[0052] (2) Treatment at 25°C during the day (8:00-16:00, 8 hours in total), and 15°C at night (16:00-8:00, 16 hours in total) (group II);

[0053] (3) Treatment at 25°C during the day (8:00-16:00, 8 hours in total), and 20°C at night (16:00-8:00, 16 hours in total) (group III);

[0054] (4) Treatment at 28°C during the day (8:00-16:00, 8 hours in total), and 15°C at night (16:00--8:00, 16 hours in total) (Group IV);

[0055] (5) Treatment at 30°C during the day (8:00-14:00, 6 hours in total), and 10°C at night (14:00-6:00, 16 hours in total) (group V);

[0056] (6) Treatment at 30°C during the day (8:00-16:00, 8 hours in total), and 15°C at night (16:00--8:00, 16 hours in total) (group VI);

[0057] (7) Treatment at 30°C during the day (8:00-16:00, 8 hours in total), and 20°C at night (16:00-8:00, 16 hours in total) (group VII);

[0058] Each of the above-mentioned diurnal temperature-variable treatment groups uses 50 seeds, and each group does 4 repetitions. Various treatments are all carried out germination tests under dark conditions, and the germination status of the seeds is observed within 90 days; Count the number of sprouts;

[0059] 2. Test results

[0060] Germination rate (G1)=(number of germinated seeds/number of seeds)×100%;

[0061] The results of the germination rate of each condition take the average value of 4 groups.

[0062] The test results are shown in Table 2 and Table 3.

[0063] The germination rate of each group is represented by the mean value of 4 groups of parallel test results.

[0064] Table 2 constant temperature conditions process the germination rate (unit: %) of Iris seed

[0065] 15℃

[0066]The results of the data in Table 2 show that the germination of the seeds of Iris japonica treated under constant temperature conditions is extremely unsatisfactory, and the germination rate is very low. Under the conditions of 15°C and 30°C, even no seeds germinate. Under the condition of 25°C, the germination rate is the highest, but It is also only 7.0%. The test results show that the germination rate of the seeds of Iris japonica under constant temperature conditions is very low, or even no germination.

[0067] The germination rate (unit: %) of table 3 temperature-changing day and night treatment Iris seed

[0068] Group I

[0069] The results of the data in Table 3 show that, compared with the constant temperature treatment conditions, the germination rate of Sinensis seeds has been significantly improved under the conditions of diurnal temperature changes, but overall, under the conditions of diurnal temperature changes, the germination rate of Iris seeds has been significantly improved. It is not ideal enough and needs to be improved.

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PUM

Property	Measurement	Unit
Concentration	50.0 ~ 200.0	m
Diameter	12.0	mm
Diameter	12.0	m
tensile		MPa
Particle size		Pa
strength	10

Description & Claims & Application Information

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Improve germination rate
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Method for improving iris lactea seed germination rate

A germination rate and seed technology, applied in the field of garden plants, can solve the problems of human hazards, high cost and complicated operation in the surrounding environment, and achieve the effects of improving the germination rate, easy control and simple operation.

AI-Extracted Technical Summary

Problems solved by technology

Method used

Abstract

Examples

Test Example

Test Example

Test Example

PUM

Description & Claims & Application Information

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