Method of producing organization engineered cartilage with inner support

A technology of tissue engineering and cartilage, applied in the direction of bone implants, medical science, prosthesis, etc., can solve problems that have not been seen yet, and achieve the effect of adapting to clinical needs, convenient material extraction, and strong proliferation ability

Inactive Publication Date: 2008-03-05
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there has been no report on the preparation of tissue-engineered cartilage with inner support by using autologous BMSCs as seed cells.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Construction of tissue-engineered cartilage with inner support for auricular prosthesis

[0019] 1. Isolation and culture of BMSCs and preparation of cell suspension:

[0020] Under aseptic conditions, 40ml of piglet iliac and femoral bone marrow were extracted by puncture, centrifuged and washed twice with serum-free DMEM culture medium, added with low-sugar DMEM culture medium containing 10% fetal bovine serum to make a cell suspension, and counted nucleated cells. 7.5×10 5 piece / cm 2 The density was inoculated on a Petri dish (Falcon, USA) and placed at 37°C, 5% CO 2 , cultured under the condition of saturated humidity, using the whole bone marrow adherent culture method to isolate BMSCs, the medium was changed for the first time 5 days after inoculation, and the suspended red blood cells were removed by rinsing twice with phosphate buffered saline (PBS). After the cells reached 80%-90% confluence, they were digested with 0.25% trypsin + 0.02% EDTA, and...

Embodiment 2

[0025] Example 2: Construction of tissue-engineered cartilage with in-band support for penile prosthesis

[0026] The methods of cell acquisition, scaffold pretreatment and cell-scaffold composite culture were the same as in Example 1, and the inner support MEDPOR was changed to 6 mm in diameter and 30 mm in length.

[0027] Various prostheses can be prepared by using the above method as needed, such as nasal dorsum, craniomaxillofacial bone / testis and other prostheses.

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Abstract

The present invention relates to medical tissue engineering, and is process of preparing tissue engineering cartilage with inner support for use as permanent prosthesis clinically. The process includes the following steps: making inner support with nondegradable stable medical porous high-density polyethylene MEDPOR, coating the inner support with nonwoven net of degradable polyglycolic acid (PGA) with high biocompatibility to form compound MEDPOR-PGA rack, dropping prepared autologous marrow matrix stem cell suspension to the nonwoven PGA net, in vitro culturing to the inducing solution with DMEM cartilage connecting TGF-beta1,IGF-I and insulin for certain time, and implanting to under skin for 4-6 weeks to constitute the tissue engineering cartilage. The present invention makes it possible to prepare clinically essential permanent implant.

Description

technical field [0001] The invention relates to the technical field of medical tissue engineering, and relates to a preparation method for constructing tissue-engineered cartilage in a common form and with inner support by using bone marrow stromal stem cells. Background technique [0002] In the field of plastic surgery and other deformity trauma repair, the reshaping of various parts of the human body often requires the implantation of intra-row supports to achieve the purpose of plastic correction, especially for craniofacial, auricle, nasal dorsum, penis, testis, etc. It is particularly important to repair the deformity or defect (loss). Traditional clinical implants mainly include: autologous tissue, allogeneic tissue, and synthetic implants, but these repair methods all have many shortcomings. There are many restrictions on obtaining autologous tissue, requiring several or several operations on several parts, which often increases the pain of the patient, secondary de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/16A61F2/28
Inventor 江华朱䴕吴玉家
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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