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Method for producing 1, trimethylene glycol by feedback control of substrate concentration with intermediate metabolites

A substrate concentration, feedback control technology, applied in biochemical equipment and methods, biomass pretreatment, bioreactor/fermenter combination, etc., can solve the problems of affecting production control, low fermentation yield, etc. Simple to use effects

Inactive Publication Date: 2010-09-29
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In view of the existing production of 1,3-propanediol has the defects of delay, blindness, influence on production control, and low fermentation yield when feeding materials, the purpose of the present invention is to overcome these defects and provide a method that can control the fermentation process in real time, Method for producing 1,3-propanediol with improved fermentation yield

Method used

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  • Method for producing 1, trimethylene glycol by feedback control of substrate concentration with intermediate metabolites
  • Method for producing 1, trimethylene glycol by feedback control of substrate concentration with intermediate metabolites
  • Method for producing 1, trimethylene glycol by feedback control of substrate concentration with intermediate metabolites

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preparation example Construction

[0056] 2. Preparation of seed solution:

[0057] Prepare the seed medium and sterilize it at 116°C for 25 minutes; insert the strain into the seed medium, and cultivate it at a suitable temperature for facultative anaerobic culture to the mid-logarithmic phase (about 12 hours after inoculation);

[0058] 3. Anaerobic fermentation: put the seed liquid prepared in step 2 into the fermentation medium sterilized at 116° C. for 25 minutes in a fermenter, control the appropriate temperature, stir, and not ventilate. During the fermentation process, as the substrate is converted into 1,3-propanediol and intermediate metabolites lactic acid and acetic acid, the pH of the fermentation broth decreases. When the pH of the fermentation broth drops below the set value, an alkaline solution is added to adjust the pH to rise To the set value, at the same time link the substrate feeding pump, add carbon source substrates (glucose, glycerol), and ferment for about 30 hours until the 1,3-propan...

Embodiment 1

[0060] Embodiment 1: (neutralizing agent base: the KOH of 4mol / L, starting substrate: glucose 8g / L, glycerol 10g / L, inoculum size: 5%, pH setting value: 7.0, fermentation temperature: 37 ℃, The added substrate in the feed solution is a mixture of glycerol, glucose and water, wherein the concentration of glycerol is 600g / L, and the concentration of glucose is 75g / L. The pre-control constant glycerol concentration is 10±5g / L, and the feedback control means: manual)

[0061] Material:

[0062] Microbial strains: Klebsiella pneumoniae, CGMCC No.1.1526 Medium:

[0063] 1) Inclined solid medium: glucose 8g / L, glycerol 10g / L, yeast powder 3g / L, K 2 HPO 4 ·3H 2 O3.4g / L, KH 2 PO 4 1.3g / L, (NH 4 ) 2 SO 4 4g / L, MgSO 4 0.24g / L, CaCO 3 0.5g / L, fumaric acid 25mg / L, agar 15g / L.

[0064] 2) Seed medium: glucose 8g / L, glycerol 10g / L, yeast powder 3g / L, K 2 HPO 4 ·3H 2 O3.4g / L, KH 2 PO 4 1.3g / L, (NH 4 ) 2 SO 4 4g / L, MgSO 4 0.24g / L, CaCO 3 0.5g / L, fumaric acid 25mg / L.

[00...

Embodiment 2

[0086] Material:

[0087] Microbial strains: Klebsiella pneumoniae, CGMCC No.1.1639

[0088] Neutralizing agent: the mixture of potassium hydroxide and ammoniacal liquor, wherein, potassium hydroxide is 4mol / L, and ammoniacal liquor (17%) consumption is 85.72g / L (as NH 3 The meter concentration is 25%-28%).

[0089] Supplementary substrate: a mixture of glycerol, glucose and water, wherein the concentration of glycerol is 600g / L, and the concentration of glucose is 75g / L.

[0090] Culture medium: basically the same as in Example 1, the air ventilation rate is controlled at 0.6L / (L·min), wherein the glucose is 5g / L, and the glycerol is 20g / L.

[0091] In this system, A=0.6849.

[0092] method:

[0093] 1, prepare seed: same as embodiment 1

[0094] 2. Fed-batch fermentation culture:

[0095] 1) Put 2 L of fermentation medium into a 5L fermentation tank, inoculate the secondary seed liquid after sterilization, the inoculation amount is 20%, install temperature electrodes a...

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Abstract

The invention provides a method for producing 1,3-propylene glycol with a fed-batch, and comprises a standard preparation method for seeds, followed by fermentation and cultivation. During fermentation, the relational function on the consumed dosage between the medium metabolite neutralizer and the substrate is used to control the concentration of the substrate (glycerol) in the fermentation liquid within a certain range, through adding the dosage of the substrate based on the feedback linkage with the consumed dosage of the medium metabolite neutralizer, so that neither substrate inhibition nor scarcity appears. The method can accomplish accurate feedback control on the substrate concentration of the fermentation liquid through monitoring the consumed dosage of the medium metabolite neutralizer, thereby increasing the yield of 1,3-propylene glycol. Control with singlechip or computer can be carried out during fermentation, with manual or automatic manipulation, while the investment is low with both simple operation and suitability for industrialized production.

Description

technical field [0001] The invention belongs to the field of chemical raw material production and relates to a method for producing 1,3-propanediol, in particular to a production method for controlling the concentration of a substrate (carbon source) through feedback of the consumption of an intermediate metabolite neutralizer. Background technique [0002] 1,3-Propanediol is an important chemical raw material. It can be used as a synthetic raw material for antifreeze, various plasticizers, detergents, preservatives and emulsifiers, and can also be used in food, cosmetics and pharmaceutical industries. Its main use is to synthesize polyester PTT (trimethylene terephthalate) as a monomer. PTT is a high-performance, easy-to-process new thermoplastic polyester material with excellent elastic refolding and low-temperature dyeing properties. In recent years, the excellent market prospect of PTT's wide application has greatly promoted the research on the production method of 1,3-p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/18C12M1/36
Inventor 谭天伟吴家鑫李政王凤寰
Owner BEIJING UNIV OF CHEM TECH
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