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Method for extracting chrysanthemum total flavone

An extraction method and technology of total flavonoids, which are applied in pharmaceutical formulations, antidote, plant raw materials, etc., can solve the problems of excessive solvent residues in extracts, difficult to reach medicinal standards, etc., and achieve good reproducibility, easy operation, and adsorption capacity. big effect

Active Publication Date: 2008-04-30
北京荃方生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestic macroporous resin extraction, the extract solvent residue is more, it is difficult to reach the medicinal standard, the present invention can overcome these shortcomings

Method used

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  • Method for extracting chrysanthemum total flavone
  • Method for extracting chrysanthemum total flavone
  • Method for extracting chrysanthemum total flavone

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0025] ③ Preparation of reference solution

[0026] Accurately weigh 9.4mg of luteolin reference substance, put it in a 25ml measuring bottle, dissolve in methanol and dilute to the mark, shake well to obtain the reference substance solution.

[0027] ④Working curve making:

[0028] Accurately draw 0.1, 0.4, 0.8, 1.2, 1.6ml into a 25ml measuring bottle, add methanol to dilute to the mark, and shake well. Using methanol as a blank, the absorbance was measured at a wavelength of 348 nm. With the absorbance as the ordinate and the concentration of the luteolin reference substance as the abscissa, draw a standard curve, and calculate the regression equation as: Y=43.098X-0.0117, R2=0.9999.

[0029] With the concentration (mg / ml) as the abscissa and the absorbance Y value as the ordinate, perform linear regression, and the regression equation is: Y=3590.7X-188.06 (r=0.9999)

[0030] The results showed that the concentration of luteolin showed a good linear relationship in the ra...

Embodiment 1

[0035] Embodiment 1 A kind of purification method of the present invention

[0036] (1) Extraction of chrysanthemum: chrysanthemum was heated and refluxed three times with 40% ethanol at 12 times, 8 times, and 8 times volume respectively, each time for 1 hour, combined three times of filtrate, concentrated under reduced pressure to the quality of medicinal material: volume was 1:2, filtered, and set aside .

[0037] (2) Loading: Put the chrysanthemum extract on the column, control the flow rate of the effluent to 0.5BV / h, and the loading amount is the weight of the medicinal material: the volume of the resin is 1.5:1, until the extract completely enters the resin bed.

[0038] (3) Elution: flush the resin bed with 5BV of 20% ethanol, and control the flow rate of the effluent to 2BV / h to remove impurities. Then use 5BV volume of 75% ethanol, control the flow rate to 2BV / h, collect the eluate, concentrate under reduced pressure to a certain volume, and spray dry to obtain the p...

Embodiment 2

[0039] Embodiment 2 selects different conditions to extract

[0040] Adopt the method provided by the present invention, select different conditions to carry out chrysanthemum total flavonoids extraction experiment, take 20g of chrysanthemum medicinal material respectively, 9 parts, carry out orthogonal test respectively by the factor level of table 1, extract 1 hour each time, the filtrate after extraction merges, Concentrate under reduced pressure to dryness, vacuum-dry to constant weight, accurately weigh an appropriate amount of dry cream, dissolve it in a 10ml measuring bottle with methanol, dilute to the mark with methanol, and shake well. Pipette 1.0ml of the filtrate into a 10ml volumetric flask, add methanol to dilute to the mark, and shake well. The content of total flavonoids was determined according to the above method, and analysis of variance was performed on the results. The results are shown in Tables 2 and 3.

[0041] Table 1 Chrysanthemum Extraction Orthogon...

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Abstract

The invention discloses a method for extracting total flavonoids from chrysanthemums. The method is to extract the chrysanthemums with ethanol, and then use a macroporous resin adsorption method to put the extract on a column, elute, collect the eluate, concentrate and dry to obtain the total flavonoids of chrysanthemums. The flavonoid approach. The method for extracting total flavonoids from chrysanthemums involved in the present invention is a method with simple and quick operation. The selected macroporous resin has the characteristics of large adsorption capacity and high desorption rate. The method of the invention uses ethanol as a solvent, which is cheap and nontoxic. The content of the total flavonoids extracted from the chrysanthemum by the method of the invention can reach more than 55%, and the reproducibility is good, and the resin can be used repeatedly.

Description

technical field [0001] The invention relates to a method for extracting traditional Chinese medicines, and mainly relates to a method for extracting effective components of traditional Chinese medicines, in particular to a method for extracting total flavonoids from chrysanthemums. Background technique [0002] Chrysanthemum: This product is the dry flower head of Chrysanthe mum morifklium Ramat. Harvest in batches when the flowers are in full bloom from September to November, dry in the shade or cultivated, or smoked, steamed and sun-dried. According to different places of origin and processing methods, medicinal materials are divided into "Haoju", "Chuju", "Gongju" and "Hangju". [0003] Chrysanthemum, sweet and bitter in taste, slightly cold, enters the Liver Meridian, improves eyesight, clears head wind, removes cloudy eyes, and releases. It can calm liver fire, internal wind, and treat dizziness and eye swelling, partial head wind, dark eyes and cloudy eyes, and red a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P9/10A61P9/12A61P39/06A61P3/06A61P31/12A61P31/18A61P31/10
Inventor 郭颂
Owner 北京荃方生物科技有限公司
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