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Total glycosides of ranunculus and preparation and application thereof

A technology of ranunculus and total glycosides, which is applied in the field of medicine to achieve the effects of strong activity, low toxicity and side effects, and high selectivity

Inactive Publication Date: 2008-04-30
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For the above diseases, although there are many therapeutic drugs, based on the curative effect and adverse reactions, looking for new drugs in this area is still a current and future research hotspot

Method used

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  • Total glycosides of ranunculus and preparation and application thereof
  • Total glycosides of ranunculus and preparation and application thereof
  • Total glycosides of ranunculus and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Preparation, composition identification and content determination of ranunculosides

[0034] 1. Preparation

[0035] (1) 2kg of the whole ranunculus herb was dried and chopped, extracted three times with 14kg of water or 10-95% ethanol or methanol under reflux or percolation, filtered through a 80-mesh sieve, combined the extracts, recovered the solvent under reduced pressure, and concentrated to 1.05kg.

[0036] (2) Add ethyl acetate equal to the volume of the concentrated solution to the concentrated solution of (1) and extract for 4 times until the ethyl acetate layer is light green, remove the lower aqueous layer and evaporate the solvent to obtain 200 g of paste;

[0037] (3) 200g paste is adsorbed by D101 macroporous resin, washed with 50% ethanol to 95% methanol, concentrated with 5-10L of alcohol, and evaporated to dryness by conventional methods under normal pressure or reduced pressure to obtain buttercup buttercup The total glycosides product is 100g, and th...

Embodiment 2

[0048] Anti-cardiac hypertrophy effect experiment of the present invention

[0049] 1. Drugs

[0050] Angiotensin II, Sigma, lot number: 125K51011

[0051] DMEM dry powder, Gibco company, batch number: 1324945

[0052] Fetal bovine serum, Hyclone company, batch number: 20060404

[0053] Trypsin, Beijing Dingguo, lot number: DH355-2

[0054] Type II collagenase, Invitrogen, lot number: DH071

[0055] Penicillin, Sigma, lot number: DH231-1

[0056] Streptomycin, Sigma, lot number: DH325

[0057] 5-BrdU, Roche, lot number: 280879

[0058] Sulforhodamine B, Sigma-Aldrich, Lot: 3520-42-1

[0059] Trichloroacetic acid, Genebase, lot number: G2365

[0060] All other reagents were domestic analytically pure

[0061] 2. Instrument

[0062] CO 2 Incubator, Haier Company;

[0063]Microplate reader 850, Biotech laboratories;

[0064] Biological inverted microscope XSP-15C, Shanghai Changfang Optical Instrument Co., Ltd.;

[0065] Low-speed centrifuge LD4-2A, Beijing Medical ...

Embodiment 3

[0103] Ranunculosides Inhibit Cardiac Effect Experiment

[0104] 1 Apparatus, Drugs and Animals

[0105] 1.1 Instrument

[0106] Biological signal acquisition system (MS4000U), Guangzhou Longfeida Technology Co., Ltd. 10g tension transducer, Beijing Xinhang Xingye Technology and Trade Co., Ltd.

[0107] 1.2 Drugs

[0108] Propranol Hydrochloride Tablets, Shaanxi Yongshou Pharmaceutical Co., Ltd., batch number: 20051028 Isoproterenol Hydrochloride Injection, Shanghai Hefeng Pharmaceutical Co., Ltd., batch number: 5E20004

[0109] 1.3 Animals

[0110] Frogs, about 60-90g, for both ♀♂ and 30 pieces, provided by Guangzhou Suibei Animal Center;

[0111] 2 Experimental methods

[0112] 2.1 Frog heart intubation:

[0113] Refer to the existing experimental method to make an isolated heart specimen of Stuart, then clamp the apex of the heart with a frog heart clip, connect the biological signal acquisition system through the tension transducer, add 1ml of Ren's solution to each ...

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Abstract

The invention discloses ranunculosides, which are extracted from whole herb of ranunculus and contain 60% to 90% of triterpene saponins. The invention also provides a preparation method and application of the ranunculosides. At the same time, it provides the application of the total ranunculoside in the preparation of cardiovascular drugs, the application in the preparation of anti-myocardial hypertrophy drugs, drugs for relaxing blood vessel smooth muscle, drugs for inhibiting heart action, analgesic and anti-inflammatory drugs, and the like. Further research on ranunculosides is expected to discover new cardiovascular and analgesic and anti-inflammatory drugs with high selectivity, strong activity, and less toxic and side effects, which will lay a solid foundation for the wide application of ranunculosides in medicine and has important Potential industrial development value and a major contribution to human research on new natural medicines.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to ranunculosides, a ranunculus whole herb extract, a preparation method thereof and an application in the field of medicine. Background technique [0002] Diseases such as cardiovascular disease and pain inflammation are common clinical diseases. Myocardial hypertrophy is a target organ response to chronic pressure or volume overload, which can lead to decreased myocardial flexibility, cardiac systolic insufficiency, serious cardiovascular events, and increased mortality and disability rates; [0003] Inflammation is a defense response of living tissue with vascular system to injury factors, and its local clinical features are redness, swelling, heat, pain and dysfunction. [0004] Although there are many therapeutic drugs for the above diseases, finding new drugs in this area is still a current and future research hotspot based on the curative effect and adverse reactions. [0...

Claims

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Application Information

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IPC IPC(8): A61K36/71A61P29/00
Inventor 谭毓治周光雄王榕乐
Owner GUANGDONG PHARMA UNIV
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