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Manganese diagnosis/measuring reagent kit and method for measuring manganese concentration

A method for determining manganese concentration and a technique for manganese concentration, applied in the field of manganese concentration determination, manganese diagnosis/measurement kit

Inactive Publication Date: 2008-05-07
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Neutron excitation analysis (NAA) is the best method, but can only be determined in some well-conditioned laboratories

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] The manganese diagnostic / measurement reagent in this embodiment is a single reagent, including:

[0091] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0092] Stabilizer 50mmol / L

[0093] Oxalate oxidase 16000U / L

[0094] Peroxidase 30000U / L

[0095] Oxalic acid 50mmol / L

[0096] 4-amino anti-arsenic 2mmol / L

[0097] Carbonic acid 10mmol / L

[0098] After all the reagents are dissolved and prepared, they are divided into bottles and freeze-dried to prepare dry powder reagents; before use, add pure water and use after reconstitution.

[0099] Set on the automatic biochemical analyzer: temperature 37℃, reaction time 10 minutes, test main wavelength 505nm, test subwavelength 600nm, the volume ratio of the tested manganese sample to reagent is 1 / 25, and the reaction direction is positive reaction (rising Reaction), the detection method is the rate method, the delay time is about 1 minute, and the detection time is about 2 minutes.

[0100] After adding the ...

Embodiment 2

[0102] The manganese diagnostic / measurement reagent in this embodiment is a dual reagent, including:

[0103] Reagent 1

[0104] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0105] Stabilizer 50mmol / L

[0106] Oxalic acid 20mmol / L

[0107] 2,4,6-Sabromo-3-hydroxy-benzenesulfonic acid 0.2mmol / L

[0108] Reagent 2

[0109] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0110] Stabilizer 50mmol / L

[0111] Oxalate oxidase 24000U / L

[0112] Peroxidase 25000U / L

[0113] 4-amino anti-arsenic 0.6mmol / L

[0114] After all the reagents are dissolved and prepared, they are divided into bottles to form a liquid double reagent, which can be used directly.

[0115] Set on the automatic biochemical analyzer: temperature 37℃, reaction time 10 minutes, test main wavelength 546nm, test sub-wavelength 600nm, the volume ratio of the tested manganese sample to reagent is 1 / 20, the reaction direction is positive reaction (rising Reaction), the detection met...

Embodiment 3

[0118] The manganese diagnosis / measurement reagent in this embodiment is three reagents, including:

[0119] Reagent 1

[0120] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0121] 3-methyl-2-benzothiazolone hydrazone 0.6mmol / L

[0122] Reagent 2

[0123] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0124] Oxalic acid 20mmol / L

[0125] Dimethylaniline 2mmol / L

[0126] Reagent 3

[0127] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0128] Stabilizer 50mmol / L

[0129] Oxalate oxidase 32000U / L

[0130] Peroxidase 20000U / L

[0131] After all the reagents are dissolved and prepared, they are divided into bottles to form three liquid reagents, which can be used directly.

[0132] Set on the automatic biochemical analyzer: temperature 37℃, reaction time 10 minutes, test main wavelength 578nm, test sub-wavelength 660nm, the volume ratio of the tested manganese sample to reagent is 1 / 30, and the reaction direction is po...

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PUM

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Abstract

The invention relates to a manganese diagnosis / determination reagent kit which adopts the techniques of enzymatic colorimetric method and enzyme couple reaction, belonging to the technical field of medicine / food / environment test and determination. The invention also relates to the method principle of determining the manganese concentration, and the reagent composition and components. The component of the reagent kit mainly comprises buffer solution, oxalic acid, oxalate oxidase, peroxidase, reduced chromogen combination, and stabilizer. The method for determining the manganese concentration is as follow: oxidizing the colorless reduced chromogen combination into colored dye through a series of enzymatic reactions; determining the dye content at the 400 to 700 nm wavelength with a visible light analyzer; finally determining the manganese concentration directly.

Description

Technical field [0001] The present invention relates to a manganese diagnosis / determination kit. At the same time, the present invention also relates to a method for determining the concentration of manganese, which belongs to the technical field of medical / food / environmental inspection and determination. Background technique [0002] As a trace element, manganese is an indispensable component in the human body, and it is also a toxic substance to the human body. In its natural state, it exists in divalent, trivalent and tetravalent forms. The lower the degree of oxidation, the higher the toxicity. Generally, manganese has eight different oxidation states. The biological significance of manganese is that it has a variety of functions as a cofactor in the human body. However, in the activation of many enzyme reactions, Mn 2+ And Mg 2+ Can replace each other. [0003] Commonly used graphite furnace atomic absorption spectrophotometry, atomic emission ICP-OES. Neutron excitation ana...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/84G01N21/78G01N21/25C12Q1/32C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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