Simple method for detecting concentration of oxalic acid

Abstract

The invention relates to a simple method for determining oxalic acid concentration. The method comprises the following steps: (1) reacting synthetic 3NO ligand with manganese perchlorate to prepare a manganese complex; (2) reacting the prepared metal manganese complex with standard hydrogen peroxide sample with concentration being 1-400 mum, determining absorbance of the reaction product at the wavelength being 450 nm, and making a standard curve of absorbance of the standard hydrogen peroxide sample at the wavelength being 450 nm; (3) reacting oxalic acid sample having unknown concentration with excessive oxalate oxidase, and decomposing the oxalic acid into equal amount of hydrogen peroxide; and (4) reacting the product of the step (3) with the metal complex prepared in the step (1), determining the absorbance of the product at the wavelength being 450 nm, and comparing with the standard curve of the absorbance of the standard hydrogen peroxide sample at the wavelength being 450 nm to obtain the concentration of the oxalic acid in the sample. The method has the advantages of simple step, high flexibility, low cost and short time.

Description

【Technical field】 [0001] The present invention relates to the technical field of concentration measurement of oxalic acid. Specifically, it uses a synthetic metal complex to react with hydrogen peroxide, a product produced by the decomposition of oxalic acid by oxalate oxidase, to generate a substance that can be absorbed by ultraviolet rays, thereby measuring oxalic acid. An easy way to concentrate. 【Background technique】 [0002] Oxalic acid is an organic substance that is produced in large quantities during cell metabolism and is toxic to almost all organisms. Its accumulation in the human body can cause various diseases. Determining the concentration of oxalic acid in blood, urine, vegetables, and plants has important theoretical significance and practical application value for the prevention, diagnosis, and treatment of oxalic acid-related diseases such as hyperoxaluria, chronic renal failure, and kidney stones. Therefore, how to simply, accurately and effectively dete...

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Problems solved by technology

[0003] Among them, the direct precipitation method and the solvent extraction method have low sensitivity; the isotope dilution method limits the application of the method in the human body because of the use of radioactive isotopes, and the sample size required for the whole process is relatively large; The small aspect has certain advantages, but it requires a long time to prepare the chromatographic column and specific instruments and equipment; the enzymatic method is mainly to determine the product CO after the decomposition of oxalic acid. 2 and hydrogen peroxide, where CO 2 It can be detected by detecting the pH value change of the buffer solution, but the reaction time is longer, and a large amount of sample (volume) is required; hydrogen peroxide can make the indicator under the catalysis of the second enzyme, such as peroxidase , such as phenol red, dimethyl adipate (DMA) and 3-methyl-2-benzothiazole hydrazone hydrochloride (MBTH) produce substances that can be absorbed by ultraviolet light, thereby indirectly detecting the concentration of oxalic acid; but this The background reading of the method is large, and after two enzymatic reactions, the error is large, but the enzymatic method is still widely used because of its fast and sensitive